The human IGHG1  (immunoglobulin heavy constant gamma 1 (G1m marker)) gene encodes the Ig gamma-1 (IgG1) chain constant (C) region. The C region contains 3 Ig-like domains including the CH1, hinge, CH2 and CH3 domains.  The hinge, CH2 and CH3 region is also commonly referred to as the Fc region.  Fc meditates the interactions with the complement components and Fc-gamma receptors (FCGRs) to induce innate immune responses such as antibody-dependent complement activation and phagocytosis, respectively. Diseases associated with IGHG1 include heavy chain deposition disease, and B-cell chronic lymphocytic leukemia (B-CLL).  In addition a chromosomal translocation t(11;14)(q13;q32) within the IGHG1 locus is found in multiple myeloma (MM). MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. MM is is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia (PCL).  The human Fc control protein is expressed as a recombinant protein that include the hinge , CH2 and CH3 domains of human IgG1 (which are identical to the Fc used in other Fc-fusion proteins).  The purified protein can be used as a negative control for other Fc-fusion proteins in a variety of biological assays. 

The human IGHG1  (immunoglobulin heavy constant gamma 1 (G1m marker)) gene encodes the Ig gamma-1 (IgG1) chain constant (C) region. The C region contains 3 Ig-like domains including the CH1, hinge, CH2 and CH3 domains.  The hinge, CH2 and CH3 region is also commonly referred to as the Fc region.  Fc meditates the interactions with the complement components and Fc-gamma receptors (FCGRs) to induce innate immune responses such as antibody-dependent complement activation and phagocytosis, respectively. Diseases associated with IGHG1 include heavy chain deposition disease, and B-cell chronic lymphocytic leukemia (B-CLL).  In addition a chromosomal translocation t(11;14)(q13;q32) within the IGHG1 locus is found in multiple myeloma (MM). MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. MM is is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia (PCL).  The human Fc control protein is expressed as a recombinant protein that include the hinge , CH2 and CH3 domains of human IgG1 (which are identical to the Fc used in other Fc-fusion proteins).  The purified protein can be used as a negative control for other Fc-fusion proteins in a variety of biological assays. 

 

Product Details

 

Gene Symbol: IGHG1

 

NCBI Gene ID: 3500

 

Uniprot Entry: P01857

 

Construct Details: The recombinant human Fc control protein is expressed as a 227 amino acid protein consisting of Asp104 - Lys330 region of human IGHG1  (UniProt accession #P018571), which exists as a dimer under non-reducing condition (see the gel image inserted).

 

Source:  Human cells stably expressing human Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD 

        70         80         90        100        110        120 

GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK 

       130        140        150        160        170        180 

GQPREPQVYT LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS 

       190        200        210        220 

DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK 

 

M.W.:  Calculated molecular mass 25.6 kDa; estimated by SDS-PAGE under reducing condition 30-35 kDa probably due to glycosylation

 

Calculated PI: 7.18

 

Calculated Extinction Coefficients (M-1 cm-1, at 280nm): 35785

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier free & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

  

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nucleic Acids Res. 10:4071 (1982).

2. Biochemistry 20:2361 (1981). 

3. Proc. Natl. Acad. Sci. 93:13931 (1996).

 

 

 

 

 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0029B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.