OX40, also known as CD134, ACT35, TXGP1L and TNFRSF4, is a single-pass type I transmembrane glycoprotein of the TNF receptor superfamily (TNFRSF).  OX40 is regarded as a T cell co-stimulatory molecule that coordinates with other membrane­bound co-stimulators including CD28, CD40, CD30, CD27, and 4­1BB/CD137. OX40 is absent on naïve T cells and its expression is induced after engagement of the TCR by antigen presenting cells and co-stimulation by CD40/CD40 ligand and CD28/B7. OX40 was identified as the receptor for OX40 ligand/TNFSF4.  OX40/OX40L-mediated signaling is critical for establishing or reactivating long-term T-cell immunity and breaking immune tolerance in malignancies, leading to a reactivation of anti-tumor immunity. Engagement of OX40 on activated T cells with OX40 ligand on dendritic cells promotes T cell survival and proliferation, prolongs the immune response, and enhances the transition from effector to memory T cells. OX40 also regulates cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells. Mutations in the OX40 gene are associated with immunodeficiency 16 (IMD16). Blockade of OX40 signaling with antibody or soluble receptor is efficacious in animal models of allergic airway inflammation, graft­versus­host disease and autoimmune disease. Conversely, stimulating OX40 with an agonistic antibody has shown therapeutic potential for cancer and infectious disease.

OX40, also known as CD134, ACT35, TXGP1L and TNFRSF4, is a single-pass type I transmembrane glycoprotein of the TNF receptor superfamily (TNFRSF).  OX40 is regarded as a T cell co-stimulatory molecule that coordinates with other membrane­bound co-stimulators including CD28, CD40, CD30, CD27, and 4­1BB/CD137. OX40 is absent on naïve T cells and its expression is induced after engagement of the TCR by antigen presenting cells and co-stimulation by CD40/CD40 ligand and CD28/B7. OX40 was identified as the receptor for OX40 ligand/TNFSF4.  OX40/OX40L-mediated signaling is critical for establishing or reactivating long-term T-cell immunity and breaking immune tolerance in malignancies, leading to a reactivation of anti-tumor immunity. Engagement of OX40 on activated T cells with OX40 ligand on dendritic cells promotes T cell survival and proliferation, prolongs the immune response, and enhances the transition from effector to memory T cells. OX40 also regulates cytokine production from T cells, antigen-presenting cells, natural killer cells, and natural killer T cells. Mutations in the OX40 gene are associated with immunodeficiency 16 (IMD16). Blockade of OX40 signaling with antibody or soluble receptor is efficacious in animal models of allergic airway inflammation, graft­versus­host disease and autoimmune disease. Conversely, stimulating OX40 with an agonistic antibody has shown therapeutic potential for cancer and infectious disease.

  

Product Details

 

Gene Symbol: OX40; CD134; ACT35; TNFRSF4; IMD16; TXGP1L

 

NCBI Gene ID: 7293

 

Uniprot Entry: P43489

 

Construct Details: Expressed as the combination of a heavy chain (HC) containing VH from anti-human OX40/CD134 mAb and CH region of rabbit IgG and a light chain (LC) encoding VL from anti-OX40/CD134 mAb.  Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

 

Source: Mammalian cells stably expressing anti-OX40/CD134 heavy chain and light chain (in rabbit IgG format) and growing in chemical-defined media with no animal components. 

 

M.W.: Calculated molecular mass (kDa): 147 (49 kDa for a single HC, 24.5 kDa for a single LC); Estimated by SDS-PAGE under non-reducing condition(kDa): 150

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 1.0 mg/ml in sterile PBS pH7.4 without any carrier protein or preservative (the concentration determined by UV absorption with an A280nm of 1.0 for 0.73 mg/ml of IgG and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human OX40 protein (SKU#FCL0103) with high affinity (K_D < 1 nM) by a functional ELISA (see Technical Data). Recognizes cell surface human OX40/CD134 by flow cytometry, IHC staining and fluorescent microscopy. Mimics OX40 ligand-­binding to induce OX40 signaling activity and enhances T cell-dependent anti-tumor immunity in vivo.

 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. 

 

Technical Data

 

Recombinant OX40-Fc protein (SKU#FCL0103) was immobilized at 50 ng/well on a 96-well NuncMaxisorp plate for ELISA with serially diluted biotinylated anti-OX40/CD134monoclonal antibody, rabbit IgG(SKU#MAB1692B). Biotinylated anti-B7-H3/CD276 (SKU#MAB1755B) human IgG1 was used as a control to demonstrate the specificity of OX40/CD134 binding under the experimental conditions.  The binding curve was fit and apparent K_D was calculated for anti-OX40:OX40 binding to be 64 ± 3 pM.    

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Eur. J. Immunol. 24:677 (1994).

2. Mol. Cell. Biol. 18:558 (1998)

3. Nat. Med. 7:907 (2001).

4. Immunity 15:445 (2001).

5. J. Exp. Med. 210:1743 (2013).

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: MAB1692-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.