CD40 s a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF5). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. CD40 plays an essential role in mediating a broad range of immune and inflammatory responses including immunoglobulin class switching, B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular region. Interaction of CD40 with its ligand, CD40 Ligand (CD40L, also known as CD154), leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells.  CD40 ligation by CD40L promotes B cell activation and T cell­dependent humoral responses. Mutations in CD40 are associated with hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching or to mount an antibody-specific immune response. CD40 is also implicated in the pathology of multiple cardiovascular diseases, such as atherosclerosis, atherothrombosis, and restenosis. CD40/CD40L interaction may be required for amyloid-beta-induced microglial activation, an early event in Alzheimer disease pathogenesis. In addition CD40 plays important roles in both hematopoietic and epithelial cancers. CD40 has been a target for tumor immunotherapy.

CD40 s a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF5). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. CD40 plays an essential role in mediating a broad range of immune and inflammatory responses including immunoglobulin class switching, B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular region. Interaction of CD40 with its ligand, CD40 Ligand (CD40L, also known as CD154), leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells.  CD40 ligation by CD40L promotes B cell activation and T cell­dependent humoral responses. Mutations in CD40 are associated with hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching or to mount an antibody-specific immune response. CD40 is also implicated in the pathology of multiple cardiovascular diseases, such as atherosclerosis, atherothrombosis, and restenosis. CD40/CD40L interaction may be required for amyloid-beta-induced microglial activation, an early event in Alzheimer disease pathogenesis. In addition CD40 plays important roles in both hematopoietic and epithelial cancers. CD40 has been a target for tumor immunotherapy.

  

Product Details

 

Gene Symbol: p50; Bp50; CDW40; CD40LR; TNFRSF5

  

NCBI Gene ID: 958

 

Uniprot Entry: P25942

 

Construct Details: Full length human CD40 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGGTTCGTCTGCCTCTGCAGTGCGTCCTCTGGGGCTGCTTGCTGACCGCTGTCCATCCAGAACCACCCACTGCATGCAG

AGAAAAACAGTACCTAATAAACAGTCAGTGCTGTTCTTTGTGCCAGCCAGGACAGAAACTGGTGAGTGACTGCACAGAGT

TCACTGAAACGGAATGCCTTCCTTGCGGTGAAAGCGAATTCCTAGACACCTGGAACAGAGAGACACACTGCCACCAGCAC

AAATACTGCGACCCCAACCTAGGGCTTCGGGTCCAGCAGAAGGGCACCTCAGAAACAGACACCATCTGCACCTGTGAAGA

AGGCTGGCACTGTACGAGTGAGGCCTGTGAGAGCTGTGTCCTGCACCGCTCATGCTCGCCCGGCTTTGGGGTCAAGCAGA

TTGCTACAGGGGTTTCTGATACCATCTGCGAGCCCTGCCCAGTCGGCTTCTTCTCCAATGTGTCATCTGCTTTCGAAAAA

TGTCACCCTTGGACAAGCTGTGAGACCAAAGACCTGGTTGTGCAACAGGCAGGCACAAACAAGACTGATGTTGTCTGTGG

TCCCCAGGATCGGCTGAGAGCCCTGGTGGTGATCCCCATCATCTTCGGGATCCTGTTTGCCATCCTCTTGGTGCTGGTCT

TTATCAAAAAGGTGGCCAAGAAGCCAACCAATAAGGCCCCCCACCCCAAGCAGGAACCCCAGGAGATCAATTTTCCCGAC

GATCTTCCTGGCTCCAACACTGCTGCTCCAGTGCAGGAGACTTTACATGGATGCCAACCGGTCACCCAGGAGGATGGCAA

AGAGAGTCGCATCTCAGTGCAGGAGAGACAGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. EMBO J. 8:1403 (1989).

2. Annu. Rev. Immunol. 12:881 (1994).

3. J. Biol. Chem. 272:19569 (1997).

4. Proc. Natl. Acad. Sci. 97: 10395 (2000).

5. Immunol. Rev. 244:115 (2011).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0076-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.