CD40 s a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF5). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. CD40 plays an essential role in mediating a broad range of immune and inflammatory responses including immunoglobulin class switching, B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular region. Interaction of CD40 with its ligand, CD40 Ligand (CD40L, also known as CD154), leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells.  CD40 ligation by CD40L promotes B cell activation and T cell­dependent humoral responses. Mutations in CD40 are associated with hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching or to mount an antibody-specific immune response. CD40 is also implicated in the pathology of multiple cardiovascular diseases, such as atherosclerosis, atherothrombosis, and restenosis. CD40/CD40L interaction may be required for amyloid-beta-induced microglial activation, an early event in Alzheimer disease pathogenesis. In addition CD40 plays important roles in both hematopoietic and epithelial cancers. CD40 has been a target for tumor immunotherapy.

CD40 s a single-pass, type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF5). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells. CD40 plays an essential role in mediating a broad range of immune and inflammatory responses including immunoglobulin class switching, B cell development, and germinal center formation. CD40 contains 4 cysteine-rich repeats in the extracellular region. Interaction of CD40 with its ligand, CD40 Ligand (CD40L, also known as CD154), leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40L expressing cells.  CD40 ligation by CD40L promotes B cell activation and T cell­dependent humoral responses. Mutations in CD40 are associated with hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching or to mount an antibody-specific immune response. CD40 is also implicated in the pathology of multiple cardiovascular diseases, such as atherosclerosis, atherothrombosis, and restenosis. CD40/CD40L interaction may be required for amyloid-beta-induced microglial activation, an early event in Alzheimer disease pathogenesis. In addition CD40 plays important roles in both hematopoietic and epithelial cancers. CD40 has been a target for tumor immunotherapy.

  

Product Details

 

Gene Symbol: p50; Bp50; CDW40; CD40LR; TNFRSF5

  

NCBI Gene ID: 958

 

Uniprot Entry: P25942

 

Construct Details: The recombinant human CD40-Fc fusion is expressed as a 401 amino acid protein consisting of Glu21 - Arg193 region of CD40 (UniProt accession #P25942) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

   

Source: Human cells stably expressing human CD40-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

EPPTACREKQ YLINSQCCSL CQPGQKLVSD CTEFTETECL PCGESEFLDT WNRETHCHQH 

        70         80         90        100        110        120 

KYCDPNLGLR VQQKGTSETD TICTCEEGWH CTSEACESCV LHRSCSPGFG VKQIATGVSD 

       130        140        150        160        170        180 

TICEPCPVGF FSNVSSAFEK CHPWTSCETK DLVVQQAGTN KTDVVCGPQD RLRSTGTHTC  

       190        200        210        220        230        240 

PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN  

       250        260        270        280        290        300 

AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP 

       310        320        330        340        350        360 

QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL  

       370        380        390        400 

YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

 

M.W.: Calculated molecular mass (kDa): 44.7; Estimated by SDS-PAGE under reducing condition (kDa): 55-60

 

Calculated PI: 5.75

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 56515

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand CD40L/CD154 and inhibits CD40L-­induced signaling activity including NF-κB activation and proliferation of human B cells.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

1. EMBO J. 8:1403 (1989).

2. Annu. Rev. Immunol. 12:881 (1994).

3. J. Biol. Chem. 272:19569 (1997).

4. Proc. Natl. Acad. Sci. 97: 10395 (2000).

5. Immunol. Rev. 244:115 (2011).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0076-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.