CD177 is a glycosyl-phosphatidylinositol (GPI)-anchored cell surface glycoprotein that plays a role in neutrophil activation. A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed UPAR/Ly6 motif that was originally found in UPAR (Urokinase plasminogen activator surface receptor) and Ly6/CD59. The u-PAR/Ly-6 (UL) motif has 10 conserved cysteines with a defined disulfide-bonding pattern.  CD177 contains 2 copies of  UPAR/Ly6 motifs in the extracellular domain (ECD).  CD177 is highly expressed in normal bone marrow and weakly expressed in fetal liver. CD177 is detected  on neutrophils and in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). A soluble form of CD177 may be produced by proteolytic cleavage at the cell surface (shedding).  CD177 binds platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in the CD177 gene are associated with myeloproliferative diseases. Over-expression of the CD177 gene has been found in patients with polycythemia rubra vera. Autoantibodies against CD177 may result in pulmonary transfusion reactions, and CD177 may be involved in Wegener's granulomatosis.

CD177 is a glycosyl-phosphatidylinositol (GPI)-anchored cell surface glycoprotein that plays a role in neutrophil activation. A variety of GPI-linked surface glycoproteins are composed of one or more copies of a conserved domain of ~100 amino-acid residues termed UPAR/Ly6 motif that was originally found in UPAR (Urokinase plasminogen activator surface receptor) and Ly6/CD59. The u-PAR/Ly-6 (UL) motif has 10 conserved cysteines with a defined disulfide-bonding pattern.  CD177 contains 2 copies of  UPAR/Ly6 motifs in the extracellular domain (ECD).  CD177 is highly expressed in normal bone marrow and weakly expressed in fetal liver. CD177 is detected  on neutrophils and in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET). A soluble form of CD177 may be produced by proteolytic cleavage at the cell surface (shedding).  CD177 binds platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in the CD177 gene are associated with myeloproliferative diseases. Over-expression of the CD177 gene has been found in patients with polycythemia rubra vera. Autoantibodies against CD177 may result in pulmonary transfusion reactions, and CD177 may be involved in Wegener's granulomatosis.

 

Product Details

 

Gene Symbol: CD177; NB1; PRV1; HNA2A; PRV-1; HNA-2a; NB1GP

 

NCBI Gene ID: 57126

 

Uniprot Entry: Q8N6Q3

 

Construct Details: The recombinant human CD177 ECD is expressed as a 396 amino acid protein consisting of Leu22 - Glu406 region of CD1177 and a C-terminal His-tag.

   

Source: Human cells stably expressing CD177 ECD protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
LLCQFGTVQH VWKVSDLPRQ WTPKNTSCDS GLGCQDTLML IESGPQVSLV LSKGCTEAKD

        70         80         90        100        110        120
QEPRVTEHRM GPGLSLISYT FVCRQEDFCN NLVNSLPLWA PQPPADPGSL RCPVCLSMEG

       130        140        150        160        170        180
CLEGTTEEIC PKGTTHCYDG LLRLRGGGIF SNLRVQGCMP QPGCNLLNGT QEIGPVGMTE

       190        200        210        220        230        240
NCNRKDFLTC HRGTTIMTHG NLAQEPTDWT TSNTEMCEVG QVCQETLLLL DVGLTSTLVG

       250        260        270        280        290        300
TKGCSTVGAQ NSQKTTIHSA PPGVLVASYT HFCSSDLCNS ASSSSVLLNS LPPQAAPVPG

       310        320        330        340        350        360
DRQCPTCVQP LGTCSSGSPR MTCPRGTTHC YDGYIHLSGG GLSTKMSIQG CVAQPSSFLL

       370        380        390
NHTRQIGIFS AREKRDVQPP ASQHESTGHH HHHHHH

 

 

M.W.: Calculated molecular mass 42.6 kDa; estimated by SDS-PAGE under reducing condition 45-55 kDa

 

Calculated PI: 6.44

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 31075

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.