Tyrosine-protein kinase TYRO3, also known as BYK, DTK, RSE, SKY and TIF, is a single-pass type I transmembrane glycoprotein in the AXL/UFO subfamily of the Receptor Tyrosine Kinase (RTK) family. Like other family members AXL/UFO and MER, TYRO3 contains two immunoglobulin­like (Ig) domains and two fibronectin type III domains in the extracellular domain (ECD) and a cytoplasmic tyrosine kinase domain tail. The AXL/UFO family receptors are involved in regulation of immune responses, cell survival and migration as well as tumorigenesis and metastasis. By binding to its ligand, TULP1 or GAS6, TYRO3 transduces signals from the extracellular matrix into the cytoplasm.  Ligand binding induces dimerization and autophosphorylation of TYRO3 on its cytoplasmic tail that provides docking sites for downstream signaling molecules. Following surface activation, TYRO3 enhances PI3-kinase activity and activates the AKT survival pathway, leading to nuclear translocation and activation of NF-kB. TYRO3 signaling is involved in many biological processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. It also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.  TYRO3 acts as a receptor for various virus, including lassa virus, lymphocytic choriomeningitis virus and ebolavirus

Tyrosine-protein kinase TYRO3, also known as BYK, DTK, RSE, SKY and TIF, is a single-pass type I transmembrane glycoprotein in the AXL/UFO subfamily of the Receptor Tyrosine Kinase (RTK) family. Like other family members AXL/UFO and MER, TYRO3 contains two immunoglobulin­like (Ig) domains and two fibronectin type III domains in the extracellular domain (ECD) and a cytoplasmic tyrosine kinase domain tail. The AXL/UFO family receptors are involved in regulation of immune responses, cell survival and migration as well as tumorigenesis and metastasis. By binding to its ligand, TULP1 or GAS6, TYRO3 transduces signals from the extracellular matrix into the cytoplasm.  Ligand binding induces dimerization and autophosphorylation of TYRO3 on its cytoplasmic tail that provides docking sites for downstream signaling molecules. Following surface activation, TYRO3 enhances PI3-kinase activity and activates the AKT survival pathway, leading to nuclear translocation and activation of NF-kB. TYRO3 signaling is involved in many biological processes such as neuron protection from excitotoxic injury, platelet aggregation and cytoskeleton reorganization. It also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.  TYRO3 acts as a receptor for various virus, including lassa virus, lymphocytic choriomeningitis virus and ebolavirus

 

Product Details

 

Gene Symbol: TYRO3; BYK; Dtk; RSE; Rek; Sky; Tif; Etk-2

 

NCBI Gene ID: 7301

 

Uniprot Entry: Q06418

 

Construct Details: The recombinant human Tyro3-Fc protein is expressed as a 617 amino acid protein consisting of Ala41 - Trp429 region of Tyro3 and a a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing TYRO3-Fc protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
AGLKLMGAPV KLTVSQGQPV KLNCSVEGME EPDIQWVKDG AVVQNLDQLY IPVSEQHWIG

        70         80         90        100        110        120
FLSLKSVERS DAGRYWCQVE DGGETEISQP VWLTVEGVPF FTVEPKDLAV PPNAPFQLSC

       130        140        150        160        170        180
EAVGPPEPVT IVWWRGTTKI GGPAPSPSVL NVTGVTQSTM FSCEAHNLKG LASSRTATVH

       190        200        210        220        230        240
LQALPAAPFN ITVTKLSSSN ASVAWMPGAD GRALLQSCTV QVTQAPGGWE VLAVVVPVPP

       250        260        270        280        290        300
FTCLLRDLVP ATNYSLRVRC ANALGPSPYA DWVPFQTKGL APASAPQNLH AIRTDSGLIL

       310        320        330        340        350        360
EWEEVIPEAP LEGPLGPYKL SWVQDNGTQD ELTVEGTRAN LTGWDPQKDL IVRVCVSNAV

       370        380        390        400        410        420
GCGPWSQPLV VSSHDRAGQQ GPPHSRTSWS TGTHTCPPCP APELLGGPSV FLFPPKPKDT

       430        440        450        460        470        480
LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH

       490        500        510        520        530        540
QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSREEMTK NQVSLTCLVK

       550        560        570        580        590        600
GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE

       610

ALHNHYTQKS LSLSPGK

 

M.W.: Calculated molecular mass 67.3 kDa; estimated by SDS-PAGE under reducing condition 90-95 kDa

 

Calculated PI: 5.70

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 120735

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.