NCR2, also known as LY95, NKp44 and CD336, is a single-pass type I transmembrane glycoprotein that contains an extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  NCR2/NKp44/CD336 together with NCR1/NKp36/CD335 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs).  All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells.  Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously.  NCR2 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

NCR2, also known as LY95, NKp44 and CD336, is a single-pass type I transmembrane glycoprotein that contains an extracellular Immunoglobulin (Ig)-like domains and a short cytoplasmic tail.  NCR2/NKp44/CD336 together with NCR1/NKp36/CD335 and NCR3/NKp30/CD337 are activating receptors collectively termed the natural cytotoxicity receptors (NCRs).  All these receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and are primarily responsible for triggering NK cell-mediated lytic activities of human tumor cells.  Blocking any of these three NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously.  NCR2 may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

 

Product Details

 

Gene Symbol: NCR2; LY95; CD336; NKP44; NK-p44; dJ149M18.1; NCTR2

 

NCBI Gene ID: 9436

 

Uniprot Entry: O95944

 

Construct Details: The recombinant human NCR2/CD336-Fc fusion is expressed as a 399 amino acid protein consisting of Gln22- Ala192 region of NCR2/CD336 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

   

Source: Human cells stably expressing NCR2-Fc protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
QSKAQVLQSV AGQTLTVRCQ YPPTGSLYEK KGWCKEASAL VCIRLVTSSK PRTMAWTSRF

        70         80         90        100        110        120
TIWDDPDAGF FTVTMTDLRE EDSGHYWCRI YRPSDNSVSK SVRFYLVVSP ASASTQTSWT

       130        140        150        160        170        180
PRDLVSSQTQ TQSCVPPTAG ARQAPESPST IPVPSQPQNS TLRPGPAAPI ASTGTHTCPP

       190        200        210        220        230        240
CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK

       250        260        270        280        290        300
TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV

       310        320        330        340        350        360
YTLPPSREEM TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS

       370        380        390
KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK

 

 

M.W.: Calculated molecular mass 44.3 kDa; estimated by SDS-PAGE under reducing condition ~60 kDa probably due to glycosylation

 

Calculated PI: 8.54

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 70985

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.