FCRL1 (Fc receptor-like protein 1), also known as FcRH1 (Fc receptor homolog 1) and CD307a, is a single-pass type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (IgSF).  FCRL1 consists of an extracellular domain (ECD) with 3 C2-like immunoglobulin motifs, a transmembrane domain and a cytoplasmic domain with 5 immunoreceptor tyrosine-based inhibitory motifs (ITIM). FCRL1 represents one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. FCRL1 is preferentially expressed by B-cells that regulates BCR-mediated signaling responses and recruits ABL1 as the intracellular effector to enhance B-cell activation. FCRL1 may also play a role in the regulation of cancer cell growth.

FCRL1 (Fc receptor-like protein 1), also known as FcRH1 (Fc receptor homolog 1) and CD307a, is a single-pass type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (IgSF).  FCRL1 consists of an extracellular domain (ECD) with 3 C2-like immunoglobulin motifs, a transmembrane domain and a cytoplasmic domain with 5 immunoreceptor tyrosine-based inhibitory motifs (ITIM). FCRL1 represents one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. FCRL1 is preferentially expressed by B-cells that regulates BCR-mediated signaling responses and recruits ABL1 as the intracellular effector to enhance B-cell activation. FCRL1 may also play a role in the regulation of cancer cell growth.

 

Product Details

 

Gene Symbol: FCRL1; FCRH1; IFGP1; hIFGP1; IRTA5; CD307a

 

NCBI Gene ID: 115350

 

Uniprot Entry: Q96LA6

 

Construct Details: Full length human FCRL1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1290 (bp)

 

Gene Insert Sequence:  

ATGCTGCCGAGGCTGTTGCTGTTGATCTGTGCTCCACTCTGTGAACCTGCCGAGCTGTTTTTGATAGCCA
GCCCCTCCCATCCCACAGAGGGGAGCCCAGTGACCCTGACGTGTAAGATGCCCTTTCTACAGAGTTCAGA
TGCCCAGTTCCAGTTCTGCTTTTTCAGAGACACCCGGGCCTTGGGCCCAGGCTGGAGCAGCTCCCCCAAG
CTCCAGATCGCTGCCATGTGGAAAGAAGACACAGGGTCATACTGGTGCGAGGCACAGACAATGGCGTCCA
AAGTCTTGAGGAGCAGGAGATCCCAGATAAATGTGCACAGGGTCCCTGTCGCTGATGTGAGCTTGGAGAC
TCAGCCCCCAGGAGGACAGGTGATGGAGGGAGACAGGCTGGTCCTCATCTGCTCAGTTGCTATGGGCACA
GGAGACATCACCTTCCTTTGGTACAAAGGGGCTGTAGGTTTAAACCTTCAGTCAAAGACCCAGCGTTCAC
TGACAGCAGAGTATGAGATTCCTTCAGTGAGGGAGAGTGATGCTGAGCAATATTACTGTGTAGCTGAAAA
TGGCTATGGTCCCAGCCCCAGTGGGCTGGTGAGCATCACTGTCAGAATCCCGGTGTCTCGCCCAATCCTC
ATGCTCAGGGCTCCCAGGGCCCAGGCTGCAGTGGAGGATGTGCTGGAGCTTCACTGTGAGGCCCTGAGAG
GCTCTCCTCCGATCCTGTACTGGTTTTATCACGAGGATATCACCCTGGGGAGCAGGTCGGCCCCCTCTGG
AGGAGGAGCCTCCTTCAACCTTTCCCTGACTGAAGAACATTCTGGAAACTACTCCTGTGAGGCCAACAAT
GGCCTGGGGGCCCAGCGCAGTGAGGCGGTGACACTCAACTTCACAGTGCCTACTGGGGCCAGAAGCAATC
ATCTTACCTCAGGAGTCATTGAGGGGCTGCTCAGCACCCTTGGTCCAGCCACCGTGGCCTTATTATTTTG
CTACGGCCTCAAAAGAAAAATAGGAAGACGTTCAGCCAGGGATCCACTCAGGAGCCTTCCCAGCCCTCTA
CCCCAAGAGTTCACCTACCTCAACTCACCTACCCCAGGGCAGCTACAGCCTATATATGAAAATGTGAATG
TTGTAAGTGGGGATGAGGTTTATTCACTGGCGTACTATAACCAGCCGGAGCAGGAATCAGTAGCAGCAGA
AACCCTGGGGACACATATGGAGGACAAGGTTTCCTTAGACATCTATTCCAGGCTGAGGAAAGCAAACATT
ACAGATGTGGACTATGAAGATGCTATGTAA

 

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.