LAMP1 (Lysosome-associated membrane glycoprotein 1) or CD107a is a single pass type I transmembrane glycoprotein belonging to the LAMP family. LAMP1 contains 2 extracellular lumenal domains, a transmembrane and a cytoplasmic domain.  LAMP1 may present carbohydrate ligands to selectins and is implicated in tumor cell metastasis.  LAMP1 also acts as a receptor for Lassa virus protein.

LAMP1 (Lysosome-associated membrane glycoprotein 1) or CD107a is a single pass type I transmembrane glycoprotein belonging to the LAMP family. LAMP1 contains 2 extracellular lumenal domains, a transmembrane and a cytoplasmic domain.  LAMP1 may present carbohydrate ligands to selectins and is implicated in tumor cell metastasis.  LAMP1 also acts as a receptor for Lassa virus protein.

 

Product Details

 

Gene Symbol: LAMP1; CD107a; LAMPA; CD107-a; LGP120

 

NCBI Gene ID: 3916

 

Uniprot Entry: P11279

 

Construct Details: The recombinant human LAMP1-Fc fusion protein is expressed as a 582 amino acid protein consisting of Ala29 - Met382 region of LAMP1 and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

   

Source: Human cells stably expressing LAMP1-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
AMFMVKNGNG TACIMANFSA AFSVNYDTKS GPKNMTFDLP SDATVVLNRS SCGKENTSDP

        70         80         90        100        110        120
SLVIAFGRGH TLTLNFTRNA TRYSVQLMSF VYNLSDTHLF PNASSKEIKT VESITDIRAD

       130        140        150        160        170        180
IDKKYRCVSG TQVHMNNVTV TLHDATIQAY LSNSSFSRGE TRCEQDRPSP TTAPPAPPSP

       190        200        210        220        230        240
SPSPVPKSPS VDKYNVSGTN GTCLLASMGL QLNLTYERKD NTTVTRLLNI NPNKTSASGS

       250        260        270        280        290        300
CGAHLVTLEL HSEGTTVLLF QFGMNASSSR FFLQGIQLNT ILPDARDPAF KAANGSLRAL

       310        320        330        340        350        360
QATVGNSYKC NAEEHVRVTK AFSVNIFKVW VQAFKVEGGQ FGSVEECLLD ENSMSTGTHT

       370        380        390        400        410        420
CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH

       430        440        450        460        470        480
NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE

       490        500        510        520        530        540
PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF

       550        560        570        580
LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

 

 

 

M.W.: Calculated molecular mass 64.0 kDa; estimated by SDS-PAGE under reducing condition: ~85-90 kDa 

 

Calculated PI: 8.12

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  53705

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.