ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase family member 7) is a member of the nucleotide pyrophosphatase/ phosphodiesterase family that are involved in hydrolysis of extracellular nucleotides. Typically, this family ectoenzymes are single-pass type II transmembrane proteins with ATPase and ATP pyrophosphatase activities. Unlike other members, ENPP7 is a single pass type I transmembrane glycoprotein without nucleotide pyrophosphatase activity. ENPP7 is a choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine.  ENPP7 is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. ENPP7 also has phospholipase C activity and can cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF), leading to its inactivation. ENPP7 may function to protect the intestinal mucosa from inflammation and tumorigenesis.

ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase family member 7) is a member of the nucleotide pyrophosphatase/ phosphodiesterase family that are involved in hydrolysis of extracellular nucleotides. Typically, this family ectoenzymes are single-pass type II transmembrane proteins with ATPase and ATP pyrophosphatase activities. Unlike other members, ENPP7 is a single pass type I transmembrane glycoprotein without nucleotide pyrophosphatase activity. ENPP7 is a choline-specific phosphodiesterase that hydrolyzes sphingomyelin releasing the ceramide and phosphocholine.  ENPP7 is involved in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption in the gastrointestinal tract. ENPP7 also has phospholipase C activity and can cleave phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF), leading to its inactivation. ENPP7 may function to protect the intestinal mucosa from inflammation and tumorigenesis.

 

Product Details

 

Gene Symbol: ENPP7; NPP7; E-NPP 7; ALK-SMase;UNQ3077/PRO9912;

 

NCBI Gene ID: 339221

 

Uniprot Entry: Q6UWV6

 

Construct Details: Full length human ENPP7 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1377 (bp)

 

Gene Insert Sequence:  

ATGAGAGGCCCGGCCGTCCTCCTCACTGTGGCTCTGGCCACGCTCCTGGCTCCCGGGGCCGGAGCACCGGTACAAAGTCA
GGGCTCCCAGAACAAGCTGCTCCTGGTGTCCTTCGACGGCTTCCGCTGGAACTACGACCAGGACGTGGACACCCCCAACC
TGGACGCCATGGCCCGAGACGGGGTGAAGGCACGCTACATGACCCCCGCCTTTGTCACCATGACCAGCCCCTGCCACTTC
ACCCTGGTCACCGGCAAATATATCGAGAACCACGGGGTGGTTCACAACATGTACTACAACACCACCAGCAAGGTGAAGCT
GCCCTACCACGCCACGCTGGGCATCCAGAGGTGGTGGGACAACGGCAGCGTGCCCATCTGGATCACAGCCCAGAGGCAGG
GCCTGAGGGCTGGCTCCTTCTTCTACCCGGGCGGGAACGTCACCTACCAAGGGGTGGCTGTGACGCGGAGCCGGAAAGAA
GGCATCGCACACAACTACAAAAATGAGACGGAGTGGAGAGCGAACATCGACACAGTGATGGCGTGGTTCACAGAGGAGGA
CCTGGATCTGGTCACACTCTACTTCGGGGAGCCGGACTCCACGGGCCACAGGTACGGCCCCGAGTCCCCGGAGAGGAGGG
AGATGGTGCGGCAGGTGGACCGGACCGTGGGCTACCTCCGGGAGAGCATCGCGCGCAACCACCTCACAGACCGCCTCAAC
CTGATCATCACATCCGACCACGGCATGACGACCGTGGACAAACGGGCTGGCGACCTGGTTGAATTCCACAAGTTCCCCAA
CTTCACCTTCCGGGACATCGAGTTTGAGCTCCTGGACTACGGACCAAACGGGATGCTGCTCCCTAAAGAAGGGAGGCTGG
AGAAGGTGTACGATGCCCTCAAGGACGCCCACCCCAAGCTCCACGTCTACAAGAAGGAGGCGTTCCCCGAGGCCTTCCAC
TACGCCAACAACCCCAGGGTCACACCCCTGCTGATGTACAGCGACCTTGGCTACGTCATCCATGGGAGAATTAACGTCCA
GTTCAACAATGGGGAGCACGGCTTTGACAACAAGGACATGGACATGAAGACCATCTTCCGCGCTGTGGGCCCTAGCTTCA
GGGCGGGCCTGGAGGTGGAGCCCTTTGAGAGCGTCCACGTGTACGAGCTCATGTGCCGGCTGCTGGGCATCGTGCCCGAG
GCCAACGATGGGCACCTAGCTACTCTGCTGCCCATGCTGCACACAGAATCTGCTCTTCCGCCTGATGGAAGGCCTACTCT
CCTGCCCAAGGGAAGATCTGCTCTCCCGCCCAGCAGCAGGCCCCTCCTCGTGATGGGACTGCTGGGGACCGTGATTCTTC
TGTCTGAGGTCGCATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.