NPR2 (natriuretic peptide receptor 1), also known as GC-B, ANPRB and GUCY2B, is a single pass type I transmembrane glycoprotein that belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Guanylyl cyclases catalyze the production of cGMP from GTP and exist as soluble and membrane forms.  The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, are a family of cell-surface receptors with a similar topographic structure: an extracellular domain (ECD) for ligand-binding, a transmembrane (TM) domain, and an intracellular region that contains a protein kinase-like domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. NPR2 is a membrane-bound guanylate cyclase that serves as the primary receptor for the C-type natriuretic peptide NPPC/CNP hormone. NPR2 may play a role in the regulation of skeletal growth. The mutations in the NPR2 gene are associated with AMD1 (acromesomelic dysplasia 1), a skeletal disorder characterized by short stature, very short limbs and hand/foot malformations, and ECDM (epiphyseal chondrodysplasia, miura type), an overgrowth syndrome characterized by tall stature, long hands and feet.

NPR2 (natriuretic peptide receptor 1), also known as GC-B, ANPRB and GUCY2B, is a single pass type I transmembrane glycoprotein that belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Guanylyl cyclases catalyze the production of cGMP from GTP and exist as soluble and membrane forms.  The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, are a family of cell-surface receptors with a similar topographic structure: an extracellular domain (ECD) for ligand-binding, a transmembrane (TM) domain, and an intracellular region that contains a protein kinase-like domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. NPR2 is a membrane-bound guanylate cyclase that serves as the primary receptor for the C-type natriuretic peptide NPPC/CNP hormone. NPR2 may play a role in the regulation of skeletal growth. The mutations in the NPR2 gene are associated with AMD1 (acromesomelic dysplasia 1), a skeletal disorder characterized by short stature, very short limbs and hand/foot malformations, and ECDM (epiphyseal chondrodysplasia, miura type), an overgrowth syndrome characterized by tall stature, long hands and feet.

 

Product Details

 

Gene Symbol: NPR2; ANPRB; GCB; AMDM; ANPb; ECDM; GC-B; NPRB; SNSK; GUC2B; NPRBi; GUCY2B

 

NCBI Gene ID: 4882

 

Uniprot Entry: P20594

 

Construct Details: Full length human NPR2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3144 (bp)

 

Gene Insert Sequence:  

ATGGCGCTGCCATCACTTCTGCTGTTGGTGGCAGCCCTGGCAGGTGGGGTGCGTCCTCCCGGGGCGCGGA
ACCTGACGCTGGCGGTGGTGCTGCCAGAACACAACCTGAGCTATGCCTGGGCCTGGCCACGGGTGGGACC
CGCTGTGGCACTAGCTGTGGAGGCTCTGGGCCGGGCACTGCCCGTGGACCTGCGGTTTGTCAGCTCCGAA
CTGGAAGGCGCCTGCTCTGAGTACCTGGCACCGCTGAGCGCTGTGGACCTCAAGCTGTACCATGACCCCG
ACCTGCTGTTAGGTCCCGGTTGCGTGTACCCTGCTGCCTCTGTGGCCCGCTTTGCCTCCCACTGGCGCCT
TCCCCTGCTGACTGCGGGTGCTGTGGCCTCTGGTTTTTCGGCTAAGAATGACCATTATCGTACCCTGGTT
CGCACTGGCCCCTCTGCTCCCAAGCTGGGTGAGTTTGTGGTGACACTACACGGGCACTTCAATTGGACTG
CCCGTGCTGCCTTGCTGTACCTGGATGCTCGCACAGATGACCGGCCTCACTACTTCACCATCGAGGGCGT
CTTTGAGGCCCTGCAGGGCAGCAACCTCAGTGTGCAGCACCAGGTGTATGCCCGAGAGCCAGGGGGCCCC
GAGCAGGCCACCCACTTCATCCGGGCCAACGGGCGCATTGTGTATATCTGCGGCCCTCTGGAGATGCTGC
ATGAGATCCTGCTTCAGGCCCAGAGGGAGAATCTGACCAATGGGGATTATGTCTTCTTTTACCTGGATGT
CTTTGGGGAGAGTCTCCGTGCAGGCCCCACACGTGCTACAGGCCGGCCCTGGCAGGACAATCGCACCCGG
GAACAGGCCCAGGCCCTCAGAGAGGCCTTTCAGACTGTATTGGTGATCACGTACCGAGAACCCCCAAATC
CTGAGTATCAGGAATTCCAGAATCGTCTGCTGATAAGAGCCCGGGAAGACTTTGGTGTGGAGCTGGGCCC
TTCCCTGATGAACCTCATCGCTGGCTGCTTCTATGATGGGATCCTGCTATATGCTGAAGTCCTGAATGAG
ACAATACAGGAAGGAGGCACCCGGGAGGATGGACTTCGAATTGTGGAAAAGATGCAGGGACGAAGATATC
ACGGTGTAACTGGGCTGGTTGTCATGGACAAGAACAATGACCGAGAGACTGACTTTGTCCTCTGGGCCAT
GGGAGACCTGGATTCTGGGGACTTTCAGCCTGCAGCCCACTACTCGGGAGCTGAGAAGCAGATTTGGTGG
ACGGGACGGCCTATTCCCTGGGTGAAGGGGGCTCCTCCCTCGGACAATCCCCCCTGTGCCTTTGACTTGG
ACGACCCATCCTGTGATAAAACTCCACTTTCAACCCTGGCAATTGTGGCTCTGGGCACAGGAATCACCTT
CATCATGTTTGGTGTTTCCAGCTTCCTAATTTTCCGAAAGCTGATGCTGGAGAAGGAGCTGGCTAGCATG
TTGTGGCGTATTCGCTGGGAAGAACTGCAGTTTGGCAACTCAGAGCGTTATCACAAAGGTGCAGGCAGTC
GCCTCACACTGTCGCTGCGGGGATCCAGTTACGGCTCGCTCATGACAGCCCATGGGAAATACCAGATCTT
TGCCAACACCGGTCACTTCAAGGGAAATGTTGTCGCCATCAAACATGTGAATAAGAAGCGCATTGAGCTG
ACCCGGCAGGTTCTGTTTGAACTCAAACATATGAGAGATGTTCAGTTCAACCATCTCACTCGCTTCATTG
GCGCCTGCATAGACCCTCCCAACATTTGCATTGTCACTGAATACTGTCCTCGTGGGAGTTTACAGGATAT
TCTAGAAAATGACAGCATCAACTTGGACTGGATGTTTCGTTATTCACTCATTAATGACCTTGTTAAGGGC
ATGGCCTTTCTCCACAACAGCATTATTTCATCGCATGGGAGTCTCAAGTCCTCCAACTGTGTGGTGGATA
GTCGTTTTGTGCTCAAAATCACAGACTATGGCCTGGCCAGCTTCCGATCAACTGCTGAACCTGATGACAG
CCATGCCCTCTATGCCAAGAAGCTGTGGACTGCCCCAGAACTGCTCAGTGGGAACCCCTTGCCAACCACA
GGCATGCAGAAGGCTGACGTCTATAGCTTTGGGATCATCCTGCAGGAGATAGCACTTCGCAGTGGTCCTT
TCTACTTGGAGGGCCTGGACCTCAGCCCCAAAGAGATTGTCCAGAAGGTACGAAATGGTCAGCGGCCATA
TTTCCGGCCAAGCATTGACCGGACCCAACTGAATGAAGAGCTAGTTTTGCTGATGGAGCGATGTTGGGCT
CAGGACCCAGCTGAGCGGCCAGACTTTGGACAGATTAAGGGCTTCATTCGGCGCTTTAACAAGGAGGGTG
GCACCAGCATATTGGACAACCTCCTGCTGCGCATGGAACAGTATGCCAATAACTTGGAGAAGCTGGTGGA
GGAACGCACACAGGCCTATCTGGAGGAAAAACGCAAGGCTGAAGCTCTGCTCTACCAAATCCTACCCCAT
TCAGTGGCAGAGCAGTTAAAACGGGGAGAGACTGTACAGGCTGAGGCCTTTGACAGTGTTACCATCTACT
TCAGTGACATTGTTGGCTTCACAGCATTGTCAGCAGAGAGCACCCCCATGCAGGTAGTGACACTTCTTAA
TGACCTGTATACCTGCTTTGATGCCATAATTGACAACTTTGATGTCTACAAGGTGGAGACGATTGGGGAT
GCTTACATGGTGGTATCTGGCCTCCCAGGCCGAAATGGTCAACGCCATGCACCAGAAATTGCTCGTATGG
CCCTAGCATTACTAGATGCAGTTTCTTCCTTTCGCATCCGCCACCGACCCCATGACCAGCTGAGGCTACG
CATAGGGGTCCATACTGGGCCAGTCTGTGCTGGGGTTGTTGGCCTGAAGATGCCCCGTTATTGTCTTTTT
GGAGACACAGTGAACACTGCTTCTCGAATGGAGTCTAATGGTCAAGCGCTGAAGATCCATGTCTCCTCTA
CCACCAAGGATGCCCTAGATGAGCTAGGATGCTTCCAGCTAGAGCTTCGGGGGGATGTGGAAATGAAGGG
AAAAGGAAAGATGCGAACATACTGGCTCTTAGGAGAGCGGAAAGGACCTCCTGGACTCCTGTAA

 

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.