NPR1 (natriuretic peptide receptor 1), also known as GC-A, ANPRA and GUCY2A, is a single pass type I transmembrane glycoprotein that belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Guanylyl cyclases catalyze the production of cGMP from GTP and exist as soluble and membrane forms.  The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, are a family of cell-surface receptors with a similar topographic structure: an extracellular domain (ECD) for ligand-binding, a transmembrane (TM) domain, and an intracellular region that contains a protein kinase-like domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP and BNP), which are potent vasoactive hormones with a key role in cardiovascular homeostasis.  NPR1 plays an essential role in the regulation of endothelial cell senescence and vascular aging.  Upon activation by ANP or BNP, NPR1 stimulates the production of cyclic guanosine monophosphate (cGMP) that promotes vascular tone and volume homeostasis by activation of protein kinase cascade, thereby controlling blood pressure and maintaining cardiovascular homeostasis. 

NPR1 (natriuretic peptide receptor 1), also known as GC-A, ANPRA and GUCY2A, is a single pass type I transmembrane glycoprotein that belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Guanylyl cyclases catalyze the production of cGMP from GTP and exist as soluble and membrane forms.  The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, are a family of cell-surface receptors with a similar topographic structure: an extracellular domain (ECD) for ligand-binding, a transmembrane (TM) domain, and an intracellular region that contains a protein kinase-like domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP and BNP), which are potent vasoactive hormones with a key role in cardiovascular homeostasis.  NPR1 plays an essential role in the regulation of endothelial cell senescence and vascular aging.  Upon activation by ANP or BNP, NPR1 stimulates the production of cyclic guanosine monophosphate (cGMP) that promotes vascular tone and volume homeostasis by activation of protein kinase cascade, thereby controlling blood pressure and maintaining cardiovascular homeostasis. 

 

Product Details

 

Gene Symbol: NPR1; ANPa; GC-A; NPRA; ANP-A; ANPRA; GUC2A; GUCY2A

 

NCBI Gene ID: 4881

 

Uniprot Entry: P16066

 

Construct Details: Full length human NPR1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3186 (bp)

 

Gene Insert Sequence:  

ATGCCGGGGCCCCGGCGCCCCGCTGGCTCCCGCCTGCGCCTGCTCCTGCTCCTGCTGCTGCCGCCGCTGC
TGCTGCTGCTCCGGGGCAGCCACGCGGGCAACCTGACGGTAGCCGTGGTACTGCCGCTGGCCAATACCTC
GTACCCCTGGTCGTGGGCGCGCGTGGGACCCGCCGTGGAGCTGGCCCTGGCCCAGGTGAAGGCGCGCCCC
GACTTGCTGCCGGGCTGGACGGTCCGCACGGTGCTGGGCAGCAGCGAAAACGCGCTGGGCGTCTGCTCCG
ACACCGCAGCGCCCCTGGCCGCGGTGGACCTCAAGTGGGAGCACAACCCCGCTGTGTTCCTGGGCCCCGG
CTGCGTGTACGCCGCCGCCCCAGTGGGGCGCTTCACCGCGCACTGGCGGGTCCCGCTGCTGACCGCCGGC
GCCCCGGCGCTGGGCTTCGGTGTCAAGGACGAGTATGCGCTGACCACCCGCGCGGGGCCCAGCTACGCCA
AGCTGGGGGACTTCGTGGCGGCGCTGCACCGACGGCTGGGCTGGGAGCGCCAAGCGCTCATGCTCTACGC
CTACCGGCCGGGTGACGAAGAGCACTGCTTCTTCCTCGTGGAGGGGCTGTTCATGCGGGTCCGCGACCGC
CTCAATATTACGGTGGACCACCTGGAGTTCGCCGAGGACGACCTCAGCCACTACACCAGGCTGCTGCGGA
CCATGCCGCGCAAAGGCCGAGTTATCTACATCTGCAGCTCCCCTGATGCCTTCAGAACCCTCATGCTCCT
GGCCCTGGAAGCTGGCTTGTGTGGGGAGGACTACGTTTTCTTCCACCTGGATATCTTTGGGCAAAGCCTG
CAAGGTGGACAGGGCCCTGCTCCCCGCAGGCCCTGGGAGAGAGGGGATGGGCAGGATGTCAGTGCCCGCC
AGGCCTTTCAGGCTGCCAAAATCATTACATATAAAGACCCAGATAATCCCGAGTACTTGGAATTCCTGAA
GCAGTTAAAACACCTGGCCTATGAGCAGTTCAACTTCACCATGGAGGATGGCCTGGTGAACACCATCCCA
GCATCCTTCCACGACGGGCTCCTGCTCTATATCCAGGCAGTGACGGAGACTCTGGCACATGGGGGAACTG
TTACTGATGGGGAGAACATCACTCAGCGGATGTGGAACCGAAGCTTTCAAGGTGTGACAGGATACCTGAA
AATTGATAGCAGTGGCGATCGGGAAACAGACTTCTCCCTCTGGGATATGGATCCCGAGAATGGTGCCTTC
AGGGTTGTACTGAACTACAATGGGACTTCCCAAGAGCTGGTGGCTGTGTCGGGGCGCAAACTGAACTGGC
CCCTGGGGTACCCTCCTCCTGACATCCCCAAATGTGGCTTTGACAACGAAGACCCAGCATGCAACCAAGA
TCACCTTTCCACCCTGGAGGTGCTGGCTTTGGTGGGCAGCCTCTCCTTGCTCGGCATTCTGATTGTCTCC
TTCTTCATATACAGGAAGATGCAGCTGGAGAAGGAACTGGCCTCGGAGCTGTGGCGGGTGCGCTGGGAGG
ACGTTGAGCCCAGTAGCCTTGAGAGGCACCTGCGGAGTGCAGGCAGCCGGCTGACCCTGAGCGGGAGAGG
CTCCAATTACGGCTCCCTGCTAACCACAGAGGGCCAGTTCCAAGTCTTTGCCAAGACAGCATATTATAAG
GGCAACCTCGTGGCTGTGAAACGTGTGAACCGTAAACGCATTGAGCTGACACGAAAAGTCCTGTTTGAAC
TGAAGCATATGCGGGATGTGCAGAATGAACACCTGACCAGGTTTGTGGGAGCCTGCACCGACCCCCCCAA
TATCTGCATCCTCACAGAGTACTGTCCCCGTGGGAGCCTGCAGGACATTCTGGAGAATGAGAGCATCACC
CTGGACTGGATGTTCCGGTACTCACTCACCAATGACATCGTCAAGGGCATGCTGTTTCTACACAATGGGG
CTATCTGTTCCCATGGGAACCTCAAGTCATCCAACTGCGTGGTAGATGGGCGCTTTGTGCTCAAGATCAC
CGACTATGGGCTGGAGAGCTTCAGGGACCTGGACCCAGAGCAAGGACACACCGTTTATGCCAAAAAGCTG
TGGACGGCCCCTGAGCTCCTGCGAATGGCTTCACCCCCTGTGCGGGGCTCCCAGGCTGGTGACGTATACA
GCTTTGGGATCATCCTTCAGGAGATTGCCCTGAGGAGTGGGGTCTTCCACGTGGAAGGTTTGGACCTGAG
CCCCAAAGAGATCATCGAGCGGGTGACTCGGGGTGAGCAGCCCCCCTTCCGGCCCTCCCTGGCCCTGCAG
AGTCACCTGGAGGAGTTGGGGCTGCTCATGCAGCGGTGCTGGGCTGAGGACCCACAGGAGAGGCCACCAT
TCCAGCAGATCCGCCTGACGTTGCGCAAATTTAACAGGGAGAACAGCAGCAACATCCTGGACAACCTGCT
GTCCCGCATGGAGCAGTACGCGAACAATCTGGAGGAACTGGTGGAGGAGCGGACCCAGGCATACCTGGAG
GAGAAGCGCAAGGCTGAGGCCCTGCTCTACCAGATCCTGCCTCACTCAGTGGCTGAGCAGCTGAAGCGTG
GGGAGACGGTGCAGGCCGAAGCCTTTGACAGTGTTACCATCTACTTCAGTGACATTGTGGGTTTCACAGC
GCTGTCGGCGGAGAGCACACCCATGCAGGTGGTGACCCTGCTCAATGACCTGTACACTTGCTTTGATGCT
GTCATAGACAACTTTGATGTGTACAAGGTGGAGACAATTGGCGATGCCTACATGGTGGTGTCAGGGCTCC
CTGTGCGGAACGGGCGGCTACACGCCTGCGAGGTAGCCCGCATGGCCCTGGCACTGCTGGATGCTGTGCG
CTCCTTCCGAATCCGCCACCGGCCCCAGGAGCAGCTGCGCTTGCGCATTGGCATCCACACAGGACCTGTG
TGTGCTGGAGTGGTGGGACTGAAGATGCCCCGTTACTGTCTCTTTGGGGATACAGTCAACACAGCCTCAA
GAATGGAGTCTAATGGGGAAGCCCTGAAGATCCACTTGTCTTCTGAGACCAAGGCTGTCCTGGAGGAGTT
TGGTGGTTTCGAGCTGGAGCTTCGAGGGGATGTAGAAATGAAGGGCAAAGGCAAGGTTCGGACCTACTGG
CTCCTTGGGGAGAGGGGGAGTAGCACCCGAGGCTGA

 

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

 

 

Documentation

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.