TPOR (thrombopoietin receptor) or MPL (myeloproliferative leukemia protein) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). TPOR is the human homolog of oncogene, v-mpl, which was originally identified from the murine myeloproliferative leukemia virus and capable of immortalize bone marrow hematopoietic cells from different lineages. TPOR is the receptor for thrombopoietin that is the major regulator of megakaryocytopoiesis and platelet formation. TPOR consists of a 466 amino acid (aa) extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a 122 aa cytoplasmic region with a Box 1 motif. The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation. TPOR deficient mice were severely thrombocytopenic. Upon binding of thrombopoietin, TPOR is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. Mutations affecting the TPOR gene are associated with congenital amegakaryocytic thrombocytopenia (CAMT) and thrombocythemia 2 (THCYT2).

TPOR (thrombopoietin receptor) or MPL (myeloproliferative leukemia protein) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). TPOR is the human homolog of oncogene, v-mpl, which was originally identified from the murine myeloproliferative leukemia virus and capable of immortalize bone marrow hematopoietic cells from different lineages. TPOR is the receptor for thrombopoietin that is the major regulator of megakaryocytopoiesis and platelet formation. TPOR consists of a 466 amino acid (aa) extracellular region with 2 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a 122 aa cytoplasmic region with a Box 1 motif. The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation. TPOR deficient mice were severely thrombocytopenic. Upon binding of thrombopoietin, TPOR is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. Mutations affecting the TPOR gene are associated with congenital amegakaryocytic thrombocytopenia (CAMT) and thrombocythemia 2 (THCYT2).

 

Product Details

 

Gene Symbol: TPOR; MPL; CD110; MPLV; TPOR; C-MPL; THCYT2

 

NCBI Gene ID: 4352

 

Uniprot Entry: P40238

 

Construct Details: The recombinant human TPOR-Fc fusion is expressed as a 692 amino acid protein consisting of Gln26- Thr489 region of TPOR and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted).  

   

Source: Human cells stably expressing TPOR-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QDVSLLASDS EPLKCFSRTF EDLTCFWDEE EAAPSGTYQL LYAYPREKPR ACPLSSQSMP  

        70         80         90        100        110        120 

HFGTRYVCQF PDQEEVRLFF PLHLWVKNVF LNQTRTQRVL FVDSVGLPAP PSIIKAMGGS  

       130        140        150        160        170        180 

QPGELQISWE EPAPEISDFL RYELRYGPRD PKNSTGPTVI QLIATETCCP ALQRPHSASA 

       190        200        210        220        230        240 

LDQSPCAQPT MPWQDGPKQT SPSREASALT AEGGSCLISG LQPGNSYWLQ LRSEPDGISL  

       250        260        270        280        290        300 

GGSWGSWSLP VTVDLPGDAV ALGLQCFTLD LKNVTCQWQQ QDHASSQGFF YHSRARCCPR 

       310        320        330        340        350        360 

DRYPIWENCE EEEKTNPGLQ TPQFSRCHFK SRNDSIIHIL VEVTTAPGTV HSYLGSPFWI 

       370        380        390        400        410        420 

HQAVRLPTPN LHWREISSGH LELEWQHPSS WAAQETCYQL RYTGEGHQDW KVLEPPLGAR 

       430        440        450        460        470        480 

GGTLELRPRS RYRLQLRARL NGPTYQGPWS SWSDPTRVET ATETSTGTHT CPPCPAPELL  

       490        500        510        520        530        540 

GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ 

       550        560        570        580        590        600 

YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR 

       610        620        630        640        650        660 

EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS  

       670        680        690 

RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK 

 

   

M.W.: Calculated molecular mass (kDa): 77.9; Estimated by SDS-PAGE under reducing condition (kDa): ~85-90

 

Calculated PI: 6.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  145520

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.