GHR (growth hormone receptor) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). GHR is the receptor for pituitary gland growth hormone (GH) involved in regulating postnatal body growth. Upon ligand binding, GHR activates the JAK2/STAT5 signaling pathway.  GHR consists of a 246 amino acid (aa) extracellular region with 1 fibronectin type III (FN3) domain and a juxtamembrane WSXWS motif, a transmembrane domain, and a 350 aa cytoplasmic region with a Box 1 motif and an UbE (ubiquitination-dependent endocytosis) motif.  The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation while the UbE is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization. The extracellular domain of GHR can be cleaved by ADAM17/TACE to produce a soluble form of GHR, also termed GHBP (growth hormone-binding protein). GHBP acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Mutations in the GHR gene are associated with Laron syndrome (LARS), also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. 

GHR (growth hormone receptor) is a single pass type I transmembrane glycoprotein of the type I cytokine receptor family (type 1 subfamily). GHR is the receptor for pituitary gland growth hormone (GH) involved in regulating postnatal body growth. Upon ligand binding, GHR activates the JAK2/STAT5 signaling pathway.  GHR consists of a 246 amino acid (aa) extracellular region with 1 fibronectin type III (FN3) domain and a juxtamembrane WSXWS motif, a transmembrane domain, and a 350 aa cytoplasmic region with a Box 1 motif and an UbE (ubiquitination-dependent endocytosis) motif.  The WSXWS motif may be necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding.  The box 1 motif is required for JAK interaction/activation while the UbE is required for recruitment of the ubiquitin conjugation system on to the receptor and for its internalization. The extracellular domain of GHR can be cleaved by ADAM17/TACE to produce a soluble form of GHR, also termed GHBP (growth hormone-binding protein). GHBP acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Mutations in the GHR gene are associated with Laron syndrome (LARS), also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. 

 

Product Details

 

Gene Symbol: GHR; GHBP; GHIP; GH-R; LARS; GHIS

 

NCBI Gene ID: 2690

 

Uniprot Entry: P10912

 

Construct Details: Full length human GHR gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1917 (bp)

 

Gene Insert Sequence:  

ATGGATCTCTGGCAGCTGCTGTTGACCTTGGCACTGGCAGGATCAAGTGATGCTTTTTCTGGAAGTGAGG

CCACAGCAGCTATCCTTAGCAGAGCACCCTGGAGTCTGCAAAGTGTTAATCCAGGCCTAAAGACAAATTC

TTCTAAGGAGCCTAAATTCACCAAGTGCCGTTCACCTGAGCGAGAGACTTTTTCATGCCACTGGACAGAT

GAGGTTCATCATGGTACAAAGAACCTAGGACCCATACAGCTGTTCTATACCAGAAGGAACACTCAAGAAT

GGACTCAAGAATGGAAAGAATGCCCTGATTATGTTTCTGCTGGGGAAAACAGCTGTTACTTTAATTCATC

GTTTACCTCCATCTGGATACCTTATTGTATCAAGCTAACTAGCAATGGTGGTACAGTGGATGAAAAGTGT

TTCTCTGTTGATGAAATAGTGCAACCAGATCCACCCATTGCCCTCAACTGGACTTTACTGAACGTCAGTT

TAACTGGGATTCATGCAGATATCCAAGTGAGATGGGAAGCACCACGCAATGCAGATATTCAGAAAGGATG

GATGGTTCTGGAGTATGAACTTCAATACAAAGAAGTAAATGAAACTAAATGGAAAATGATGGACCCTATA

TTGACAACATCAGTTCCAGTGTACTCATTGAAAGTGGATAAGGAATATGAAGTGCGTGTGAGATCCAAAC

AACGAAACTCTGGAAATTATGGCGAGTTCAGTGAGGTGCTCTATGTAACACTTCCTCAGATGAGCCAATT

TACATGTGAAGAAGATTTCTACTTTCCATGGCTCTTAATTATTATCTTTGGAATATTTGGGCTAACAGTG

ATGCTATTTGTATTCTTATTTTCTAAACAGCAAAGGATTAAAATGCTGATTCTGCCCCCAGTTCCAGTTC

CAAAGATTAAAGGAATCGATCCAGATCTCCTCAAGGAAGGAAAATTAGAGGAGGTGAACACAATCTTAGC

CATTCATGATAGCTATAAACCCGAATTCCACAGTGATGACTCTTGGGTTGAATTTATTGAGCTAGATATT

GATGAGCCAGATGAAAAGACTGAGGAATCAGACACAGACAGACTTCTAAGCAGTGACCATGAGAAATCAC

ATAGTAACCTAGGGGTGAAGGATGGCGACTCTGGACGTACCAGCTGTTGTGAACCTGACATTCTGGAGAC

TGATTTCAATGCCAATGACATACATGAGGGTACCTCAGAGGTTGCTCAGCCACAGAGGTTAAAAGGGGAA

GCAGATCTCTTATGCCTTGACCAGAAGAATCAAAATAACTCACCTTATCATGATGCTTGCCCTGCTACTC

AGCAGCCCAGTGTTATCCAAGCAGAGAAAAACAAACCACAACCACTTCCTACTGAAGGAGCTGAGTCAAC

TCACCAAGCTGCCCATATTCAGCTAAGCAATCCAAGTTCACTGTCAAACATCGACTTTTATGCCCAGGTG

AGCGACATTACACCAGCAGGTAGTGTGGTCCTTTCCCCGGGCCAAAAGAATAAGGCAGGGATGTCCCAAT

GTGACATGCACCCGGAAATGGTCTCACTCTGCCAAGAAAACTTCCTTATGGACAATGCCTACTTCTGTGA

GGCAGATGCCAAAAAGTGCATCCCTGTGGCTCCTCACATCAAGGTTGAATCACACATACAGCCAAGCTTA

AACCAAGAGGACATTTACATCACCACAGAAAGCCTTACCACTGCTGCTGGGAGGCCTGGGACAGGAGAAC

ATGTTCCAGGTTCTGAGATGCCTGTCCCAGACTATACCTCCATTCATATAGTACAGTCCCCACAGGGCCT

CATACTCAATGCGACTGCCTTGCCCTTGCCTGACAAAGAGTTTCTCTCATCATGTGGCTATGTGAGCACA

GACCAACTGAACAAAATCATGCCTTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.