ALB (Albumin) or HSA (Human Serum Albumin) is the most abundant protein found in human blood. Human ALB gene encodes a preproprotein that is further proteolytically processed to generate the mature form.  ALB regulates blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of molecules, including hormones, bilirubin, fatty acids, and metabolites, as well as exogenous drugs. ALB is a major zinc transporter in plasma, typically binds ~80% of all plasma zinc. ALB also binds ~45% of circulating calcium and magnesium in plasma. The rank order of its affinity is zinc > calcium > magnesium.  ALB contains at least 2 calcium-binding sites and the shareds binding site between zinc and calcium at residue Asp-273. Additionally, ALB exhibits an esterase-like activity with broad substrate specificity.  ALB binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin. The mutations in the ALB gene are associated with FDAH (Familial Dysalbuminemic Hyperthyroxinemia) and ANALBA (Analbuminemia) conditions.  

ALB (Albumin) or HSA (Human Serum Albumin) is the most abundant protein found in human blood. Human ALB gene encodes a preproprotein that is further proteolytically processed to generate the mature form.  ALB regulates blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of molecules, including hormones, bilirubin, fatty acids, and metabolites, as well as exogenous drugs. ALB is a major zinc transporter in plasma, typically binds ~80% of all plasma zinc. ALB also binds ~45% of circulating calcium and magnesium in plasma. The rank order of its affinity is zinc > calcium > magnesium.  ALB contains at least 2 calcium-binding sites and the shareds binding site between zinc and calcium at residue Asp-273. Additionally, ALB exhibits an esterase-like activity with broad substrate specificity.  ALB binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin. The mutations in the ALB gene are associated with FDAH (Familial Dysalbuminemic Hyperthyroxinemia) and ANALBA (Analbuminemia) conditions.  

 

Product Details

 

Gene Symbol: ALB; Albumin; HSA; FDAHT; PRO0883; PRO0903; PRO1341

 

NCBI Gene ID: 213

 

Uniprot Entry: P02768

 

Construct Details: The recombinant human ALB protein is expressed as a 599 amino acid protein consisting of Glu25 - Leu609 region of ALB (UniProt accession #P02768) and a C-terminal His-tag. It contains 24 potential sites for N-linked glycosylation.

 

Source: Human cells stably expressing ALB and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

EASDAHKSEV AHRFKDLGEE NFKALVLIAF AQYLQQCPFE DHVKLVNEVT EFAKTCVADE 

        70         80         90        100        110        120 

SAENCDKSLH TLFGDKLCTV ATLRETYGEM ADCCAKQEPE RNECFLQHKD DNPNLPRLVR 

       130        140        150        160        170        180 

PEVDVMCTAF HDNEETFLKK YLYEIARRHP YFYAPELLFF AKRYKAAFTE CCQAADKAAC 

       190        200        210        220        230        240 

LLPKLDELRD EGKASSAKQR LKCASLQKFG ERAFKAWAVA RLSQRFPKAE FAEVSKLVTD 

       250        260        270        280        290        300 

LTKVHTECCH GDLLECADDR ADLAKYICEN QDSISSKLKE CCEKPLLGKS HCIAEVENDE 

       310        320        330        340        350        360 

MPADLPSLAA DFVESKDVCK NYAEAKDVFL GMFLYEYARR HPDYSVVLLL RLAKTYETTL 

       370        380        390        400        410        420 

EKCCAAADPH ECYAKVFDEF KPLVEEPQNL IKQNCELFEQ LGEYKFQNAL LVRYTKKVPQ 

       430        440        450        460        470        480 

VSTPTLVEVS RNLGKVGSKC CKHPEAKRMP CAEDYLSVVL NQLCVLHEKT PVSDRVTKCC 

       490        500        510        520        530        540 

TESLVNRRPC FSALEVDETY VPKEFNAETF TFHADICTLS EKERQIKKQT ALVELVKHKP 

       550        560        570        580        590 

KATKEQLKAV MDDFAAFVEK CCKADDKETC FAEEGKKLVA ASQAALGLST GHHHHHHHH 

 

 

  

M.W.: Calculated molecular mass (kDa): 68; Estimated by SDS-PAGE under reducing condition (kDa): ~75-80

 

Calculated PI: 5.94

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  34445

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.