LILRA3 (Leukocyte immunoglobulin like receptor A3), also known as CD85e and LIR4 (Leukocyte immunoglobulin-like receptor 4), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  LIR family members are known to have activating and inhibitory functions in leukocytes.  LILRA3 belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail.  LILRA3 contains 4 Ig-like C2 type domains in the extracellular region. LILRA3 acts as a receptor for class I MHC antigens. LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. LILRA3 binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

LILRA3 (Leukocyte immunoglobulin like receptor A3), also known as CD85e and LIR4 (Leukocyte immunoglobulin-like receptor 4), is a member of the leukocyte immunoglobulin (Ig)-like receptor (LIR) family of the Ig superfamily (IgSF).  LIR family members are known to have activating and inhibitory functions in leukocytes.  LILRA3 belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail.  LILRA3 contains 4 Ig-like C2 type domains in the extracellular region. LILRA3 acts as a receptor for class I MHC antigens. LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. LILRA3 binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

 

Product Details

 

Gene Symbol: LILRA3; LIR4; CD85E; LIR-4; ILT6; ILT-6; HM31; HM43

 

NCBI Gene ID: 11026

 

Uniprot Entry: Q8N6C8

 

Construct Details: The recombinant human LILRA3 ECD protein is expressed as an 644-amino acid protein consisting of Gly24 -  Glu439 region of LILRA3 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions. LILRA3 ECD contains 5 potential N-linked glycosylation sites.

 

Source: Human cells stably expressing LILRA3-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

GPLPKPTLWA EPGSVITQGS PVTLRCQGSL ETQEYHLYRE KKTALWITRI PQELVKKGQF 

        70         80         90        100        110        120 

PILSITWEHA GRYCCIYGSH TAGLSESSDP LELVVTGAYS KPTLSALPSP VVTSGGNVTI  

       130        140        150        160        170        180 

QCDSQVAFDG FILCKEGEDE HPQCLNSHSH ARGSSRAIFS VGPVSPSRRW SYRCYGYDSR 

       190        200        210        220        230        240 

APYVWSLPSD LLGLLVPGVS KKPSLSVQPG PVVAPGEKLT FQCGSDAGYD RFVLYKEWGR 

       250        260        270        280        290        300 

DFLQRPGRQP QAGLSQANFT LGPVSRSYGG QYTCSGAYNL SSEWSAPSDP LDILITGQIR 

       310        320        330        340        350        360 

ARPFLSVRPG PTVASGENVT LLCQSQGGMH TFLLTKEGAA DSPLRLKSKR QSHKYQAEFP 

       370        380        390        400        410        420 

MSPVTSAHAG TYRCYGSLSS NPYLLTHPSD PLELVVSGAA ETLSPPQNKS DSKAGESTGT 

       430        440        450        460        470        480 

HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE  

       490        500        510        520        530        540 

VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV SNKALPAPIE KTISKAKGQP 

       550        560        570        580        590        600 

REPQVYTLPP SREEMTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT TPPVLDSDGS 

       610        620        630        640 

FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGK 

 

 

  

M.W.: Calculated molecular mass (kDa): 70.6; Estimated by SDS-PAGE under reducing condition (kDa): ~85-90

 

Calculated PI: 8.20

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  101730

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Not available

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.