CD79A is also known as immunoglobulin-associated alpha (Ig-alpha or IGA) or MB-1 membrane glycoprotein. CD79A and CD79B form a disulfide-linked heterodimer, which is a part of the B-cell antigen receptor complex (BCR). BCR consists the antigen-specific component, surface Ig, where it non-covalently associates with the CD79 heterodimer.  The CD79 heterodimer is essential for expression and function of BCR.  Like CD79B, CD79A is a single-pass type I transmembrane protein of the Ig superfamily (IgSF).  It contains 1 Ig-like V-type domain in the extracellular domain (ECD) and 1 ITAM motif in the cytoplasmic tail.   CD79A is required in cooperation with CD79B for the initiation and transduction of signaling cascade by BCR, which leads to internalization of the complex, trafficking to endosomes and antigen presentation. CD79A and CD79B become phosphorylated on tyrosines upon B-cell activation by SRC-type Tyr-kinases such as BLK, LYN and SYK.  CD79A is also required for efficient differentiation of pro- and pre-B-cells while it represses BCR signaling during development of immature B-cells. Mutations in the CD79A gene are associated with Agammaglobulinemia 3, autosomal recessive (AGM3), a primary immunodeficiency characterized by profoundly low or absent serum antibodies and circulating B-cells due to an early blockade of B-cell development. 

CD79A is also known as immunoglobulin-associated alpha (Ig-alpha or IGA) or MB-1 membrane glycoprotein. CD79A and CD79B form a disulfide-linked heterodimer, which is a part of the B-cell antigen receptor complex (BCR). BCR consists the antigen-specific component, surface Ig, where it non-covalently associates with the CD79 heterodimer.  The CD79 heterodimer is essential for expression and function of BCR.  Like CD79B, CD79A is a single-pass type I transmembrane protein of the Ig superfamily (IgSF).  It contains 1 Ig-like V-type domain in the extracellular domain (ECD) and 1 ITAM motif in the cytoplasmic tail.   CD79A is required in cooperation with CD79B for the initiation and transduction of signaling cascade by BCR, which leads to internalization of the complex, trafficking to endosomes and antigen presentation. CD79A and CD79B become phosphorylated on tyrosines upon B-cell activation by SRC-type Tyr-kinases such as BLK, LYN and SYK.  CD79A is also required for efficient differentiation of pro- and pre-B-cells while it represses BCR signaling during development of immature B-cells. Mutations in the CD79A gene are associated with Agammaglobulinemia 3, autosomal recessive (AGM3), a primary immunodeficiency characterized by profoundly low or absent serum antibodies and circulating B-cells due to an early blockade of B-cell development. 

 

Product Details

 

Gene Symbol: CD79A; IGA; MB1; AGM3; Ig-alpha

 

NCBI Gene ID: 973

 

Uniprot Entry: P11912

 

Construct Details: Full length human CD79A gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- a selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 681 (bp)

 

Gene Insert Sequence:  

ATGCCTGGGGGTCCAGGAGTCCTCCAAGCTCTGCCTGCCACCATCTTCCTCCTCTTCCTGCTGTCTGCTG

TCTACCTGGGCCCTGGGTGCCAGGCCCTGTGGATGCACAAGGTCCCAGCATCATTGATGGTGAGCCTGGG

GGAAGACGCCCACTTCCAATGCCCGCACAATAGCAGCAACAACGCCAACGTCACCTGGTGGCGCGTCCTC

CATGGCAACTACACGTGGCCCCCTGAGTTCTTGGGCCCGGGCGAGGACCCCAATGGTACGCTGATCATCC

AGAATGTGAACAAGAGCCATGGGGGCATATACGTGTGCCGGGTCCAGGAGGGCAACGAGTCATACCAGCA

GTCCTGCGGCACCTACCTCCGCGTGCGCCAGCCGCCCCCCAGGCCCTTCCTGGACATGGGGGAGGGCACC

AAGAACCGAATCATCACAGCCGAGGGGATCATCCTCCTGTTCTGCGCGGTGGTGCCTGGGACGCTGCTGC

TGTTCAGGAAACGATGGCAGAACGAGAAGCTCGGGTTGGATGCCGGGGATGAATATGAAGATGAAAACCT

TTATGAAGGCCTGAACCTGGACGACTGCTCCATGTATGAGGACATCTCCCGGGGCCTCCAGGGCACCTAC

CAGGATGTGGGCAGCCTCAACATAGGAGATGTCCAGCTGGAGAAGCCGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.