CD123, also known as IL3RA/IL3R (Interleukin-3 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  CD123/IL3RA consists of an extracellular region and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD123/IL3RA is a component of the receptor complex specific for IL3. The IL3 receptor complex is a heterodimer of CD123/IL3RA and the common signal transducing subunit CD131/CSF2RB shared by the receptors for IL3, colony stimulating factor 2 (CSF2/GM-CSF), and IL5. The binding of CD123/IL3RA to IL3 depends on the beta subunit CD131. The beta subunit CD131 is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA/CD116) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y.

CD123, also known as IL3RA/IL3R (Interleukin-3 receptor subunit alpha), is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  CD123/IL3RA consists of an extracellular region and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD123/IL3RA is a component of the receptor complex specific for IL3. The IL3 receptor complex is a heterodimer of CD123/IL3RA and the common signal transducing subunit CD131/CSF2RB shared by the receptors for IL3, colony stimulating factor 2 (CSF2/GM-CSF), and IL5. The binding of CD123/IL3RA to IL3 depends on the beta subunit CD131. The beta subunit CD131 is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA/CD116) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y.

Product Details

 

Gene Symbol: IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra

 

NCBI Gene ID: 3563

 

Uniprot Entry: P26951

 

Construct Details: The recombinant human CD123 ECD protein is expressed as a 513 amino acid protein consisting of Thr19 - Ala303 region of CD123 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source: Human cells stably expressing CD123-Fc and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

TKEDPNPPIT NLRMKAKAQQ LTWDLNRNVT DIECVKDADY SMPAVNNSYC QFGAISLCEV 

        70         80         90        100        110        120 

TNYTVRVANP PFSTWILFPE NSGKPWAGAE NLTCWIHDVD FLSCSWAVGP GAPADVQYDL  

       130        140        150        160        170        180 

YLNVANRRQQ YECLHYKTDA QGTRIGCRFD DISRLSSGSQ SSHILVRGRS AAFGIPCTDK 

       190        200        210        220        230        240 

FVVFSQIEIL TPPNMTAKCN KTHSFMHWKM RSHFNRKFRY ELQIQKRMQP VITEQVRDRT 

       250        260        270        280        290        300 

SFQLLNPGTY TVQIRARERV YEFLSAWSTP QRFECDQEEG ANTRASTGTH TCPPCPAPEL 

       310        320        330        340        350        360 

LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV DVSHEDPEVK FNWYVDGVEV HNAKTKPREE 

       370        380        390        400        410        420 

QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS NKALPAPIEK TISKAKGQPR EPQVYTLPPS  

       430        440        450        460        470        480 

REEMTKNQVS LTCLVKGFYP SDIAVEWESN GQPENNYKTT PPVLDSDGSF FLYSKLTVDK 

       490        500        510 

SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGK 

 

  

M.W.: Calculated molecular mass (kDa): 58.3; Estimated by SDS-PAGE under reducing condition (kDa): 70-75

 

Calculated PI: 8.22

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  89810

  

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to anti-CD123 antibody (SKU#MAB3779)

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 66:1165-1174(1991)

2. Biochem. Biophys. Res. Commun. 208:360-367(1995) 

3. J. Biol. Chem. 284:5763-5773(2009)

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.