CD98, also known as 4F2, MDU1 and SLC3A2, is a single-pass type II transmembrane protein belonging to the SLC3A (solute carrier family 3A) transporter family. CD98 is expressed ubiquitously in all tissues with highest levels detected in kidney, placenta and testis and weakest level in thymus. As a common heavy chain, CD98 is required for the function of light chain amino-acid transporters (LAT) in sodium-independent transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. The transporter exists as a disulfide-linked heterodimer of CD98 with one of several light chains (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). CD98 plays an important role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine.  CD98 is also involved in the transport of L-DOPA across the blood-brain barrier, and thyroid hormones across the cell membrane in tissues such as placenta. CD98 is located selectively at cell-cell adhesion site.  CD98 constitutively and specifically associates with beta-1 integrins, including alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1.  CD98 may help target LAT1 and LAT2 to the plasma membrane and regulate the transport activity. 

CD98, also known as 4F2, MDU1 and SLC3A2, is a single-pass type II transmembrane protein belonging to the SLC3A (solute carrier family 3A) transporter family. CD98 is expressed ubiquitously in all tissues with highest levels detected in kidney, placenta and testis and weakest level in thymus. As a common heavy chain, CD98 is required for the function of light chain amino-acid transporters (LAT) in sodium-independent transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. The transporter exists as a disulfide-linked heterodimer of CD98 with one of several light chains (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). CD98 plays an important role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine.  CD98 is also involved in the transport of L-DOPA across the blood-brain barrier, and thyroid hormones across the cell membrane in tissues such as placenta. CD98 is located selectively at cell-cell adhesion site.  CD98 constitutively and specifically associates with beta-1 integrins, including alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1.  CD98 may help target LAT1 and LAT2 to the plasma membrane and regulate the transport activity. 

 

Product Details

 

Gene Symbol: CD98; 4F2; SLC3A2; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC

 

NCBI Gene ID: 6520

 

Uniprot Entry: P08195

 

Construct Details: The recombinant human CD98-Fc is expressed as a 648 amino acid protein consisting of Arg110 - Ala529 region of CD98 (UniProt Accession # P08195 - isoform 2) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition (see the gel image inserted).

 

Source: Human cells stably expressing human CD98-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

RELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQ

TDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWL

QAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTS

SYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVF

SYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQR

SKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKAD

LLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAASTGTHTCPPCPAPELLGGPSVFLFPPKP

KDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQ

DWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPS

DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQK

SLSLSPGK

 

M.W.: Calculated molecular mass (kDa): 71.8; Estimated by SDS-PAGE under reducing condition (kDa): 80-90

 

Calculated PI: 5.82

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  98695

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Immobilized CD98 protein binds anti-CD98 monoclonal antibody human IgG1 (SKU#MAB0205)with high affinity (K_D < 10 nM) in a functional ELISA.  Blocks CD98/LAT-mediated amino acid transporter and signaling activity.

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 84:6526 (1987).

2. Nature 395:288 (1998).

3. J. Biol. Chem. 262:9574 (1987).

4. J Biol Chem. 273: 32437 (1998).

5. EMBO J. 18: 49 (1999).

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0352-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.