CD138, also known as SDC1 (syndecan 1), is a transmembrane (type I) heparan sulfate proteoglycan of the syndecan proteoglycan family. The syndecans mediate cell adhesion, signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. CD138 is a 310 amino acid (aa) single pass type I transmembrane protein with an N-terminal signal peptide, a 232-aa extracellular domain (ECD) with 6 potential glycosylation sites, a transmembrane domain (TM), and a short 35-aa cytoplasmic tail. CD138 is a cell surface proteoglycan with both heparan sulfate and chondroitin sulfate. CD138 also regulates exosome biogenesis.  CD138 shedding is enhanced by a number of factors such as heparanase, thrombin, PMA or EGF, and also by stress and wound healing. CD138 may play a role in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. 

CD138, also known as SDC1 (syndecan 1), is a transmembrane (type I) heparan sulfate proteoglycan of the syndecan proteoglycan family. The syndecans mediate cell adhesion, signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. CD138 is a 310 amino acid (aa) single pass type I transmembrane protein with an N-terminal signal peptide, a 232-aa extracellular domain (ECD) with 6 potential glycosylation sites, a transmembrane domain (TM), and a short 35-aa cytoplasmic tail. CD138 is a cell surface proteoglycan with both heparan sulfate and chondroitin sulfate. CD138 also regulates exosome biogenesis.  CD138 shedding is enhanced by a number of factors such as heparanase, thrombin, PMA or EGF, and also by stress and wound healing. CD138 may play a role in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. 

 

Product Details

 

Gene Symbol: SDC1; SDC; CD138; SYND1; syndecan

 

NCBI Gene ID: 6382

 

Uniprot Entry: P18827

 

Construct Details: The recombinant human CD138-Fc is expressed as a 462-amino acid protein consisting of  Gln23 - Glu251 region of CD138 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.   

 

Source: Human cells stably expressing human CD138-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

QPALPQIVAT NLPPEDQDGS GDDSDNFSGS GAGALQDITL SQQTPSTWKD TQLLTAIPTS 

        70         80         90        100        110        120 

PEPTGLEATA ASTSTLPAGE GPKEGEAVVL PEVEPGLTAR EQEATPRPRE TTQLPTTHLA 

       130        140        150        160        170        180 

STTTATTAQE PATSHPHRDM QPGHHETSTP AGPSQADLHT PHTEDGGPSA TERAAEDGAS 

       190        200        210        220        230        240 

SQLPAAEGSG EQDFTFETSG ENTAVVAVEP DRRNQSPVDQ GATGASQGLL DRKESTGTHT  

       250        260        270        280        290        300 

CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH 

       310        320        330        340        350        360 

NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE 

       370        380        390        400        410        420 

PQVYTLPPSR EEMTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF 

       430        440        450        460 

LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

  

M.W.: Calculated molecular mass (kDa): 49.7; Estimated by SDS-PAGE under reducing condition (kDa): 55-85

 

Calculated PI: 4.90

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  41285

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S" and "M" for marker)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). 

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.