IL1RL2 (interleukin 1 receptor-like 2), also known as IL1Rrp2 and IL36R, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin 1 receptor (IL1R) family. The IL1R family comprises at least 10 members, which typically contain 3 immunoglobulin (Ig)-like domains in the extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL1RL1 can be induced by proinflammatory stimuli, and is involved in the function of helper T (Th) cells. IL1RL1, IL1R1, IL1R2 and IL1RL2 form a cytokine receptor gene cluster in chromosome 2q12.  IL1RL2 acts as a receptor for IL36 (IL36A, IL36B and IL36G). After binding to IL36, IL1RL2 associates with the coreceptor IL1RAP to form the IL36 receptor complex which mediates IL36-dependent activation of NF-kappa-B, MAPK and other pathways. The IL36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. IL1RL2 may be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway

IL1RL2 (interleukin 1 receptor-like 2), also known as IL1Rrp2 and IL36R, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin 1 receptor (IL1R) family. The IL1R family comprises at least 10 members, which typically contain 3 immunoglobulin (Ig)-like domains in the extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL1RL1 can be induced by proinflammatory stimuli, and is involved in the function of helper T (Th) cells. IL1RL1, IL1R1, IL1R2 and IL1RL2 form a cytokine receptor gene cluster in chromosome 2q12.  IL1RL2 acts as a receptor for IL36 (IL36A, IL36B and IL36G). After binding to IL36, IL1RL2 associates with the coreceptor IL1RAP to form the IL36 receptor complex which mediates IL36-dependent activation of NF-kappa-B, MAPK and other pathways. The IL36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. IL1RL2 may be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway

 

Product Details

 

Gene Symbol: IL1RL2; IL36R; IL-36R; IL1RRP2; IL-1Rrp2; IL1R-rp2

 

NCBI Gene ID: 8808

 

Uniprot Entry: Q9HB29

 

Construct Details: Full length human IL1RL2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGTGGTCCTTGCTGCTCTGCGGGTTGTCCATCGCCCTTCCACTGTCTGTCACAGCAGATGGATGCAAGG

ACATTTTTATGAAAAATGAGATACTTTCAGCAAGCCAGCCTTTTGCTTTTAATTGTACATTCCCTCCCAT

AACATCTGGGGAAGTCAGTGTAACATGGTATAAAAATTCTAGCAAAATCCCAGTGTCCAAAATCATACAG

TCTAGAATTCACCAGGACGAGACTTGGATTTTGTTTCTCCCCATGGAATGGGGGGACTCAGGAGTCTACC

AATGTGTTATAAAGGGTAGAGACAGCTGTCATAGAATACATGTAAACCTAACTGTTTTTGAAAAACATTG

GTGTGACACTTCCATAGGTGGTTTACCAAATTTATCAGATGAGTACAAGCAAATATTACATCTTGGAAAA

GATGATAGTCTCACATGTCATCTGCACTTCCCGAAGAGTTGTGTTTTGGGTCCAATAAAGTGGTATAAGG

ACTGTAACGAGATTAAAGGGGAGCGGTTCACTGTTTTGGAAACCAGGCTTTTGGTGAGCAATGTCTCGGC

AGAGGACAGAGGGAACTACGCGTGTCAAGCCATACTGACACACTCAGGGAAGCAGTACGAGGTTTTAAAT

GGCATCACTGTGAGCATTACAGAAAGAGCTGGATATGGAGGAAGTGTCCCTAAAATCATTTATCCAAAAA

ATCATTCAATTGAAGTACAGCTTGGTACCACTCTGATTGTGGACTGCAATGTAACAGACACCAAGGATAA

TACAAATCTACGATGCTGGAGAGTCAATAACACTTTGGTGGATGATTACTATGATGAATCCAAACGAATC

AGAGAAGGGGTGGAAACCCATGTCTCTTTTCGGGAACATAATTTGTACACAGTAAACATCACCTTCTTGG

AAGTGAAAATGGAAGATTATGGCCTTCCTTTCATGTGCCACGCTGGAGTGTCCACAGCATACATTATATT

ACAGCTCCCAGCTCCGGATTTTCGAGCTTACTTGATAGGAGGGCTTATCGCCTTGGTGGCTGTGGCTGTG

TCTGTTGTGTACATATACAACATTTTTAAGATCGACATTGTTCTTTGGTATCGAAGTGCCTTCCATTCTA

CAGAGACCATAGTAGATGGGAAGCTGTATGACGCCTATGTCTTATACCCCAAGCCCCACAAGGAAAGCCA

GAGGCATGCCGTGGATGCCCTGGTGTTGAATATCCTGCCCGAGGTGTTGGAGAGACAATGTGGATATAAG

TTGTTTATATTCGGCAGAGATGAATTCCCTGGACAAGCCGTGGCCAATGTCATCGATGAAAACGTTAAGC

TGTGCAGGAGGCTGATTGTCATTGTGGTCCCCGAATCGCTGGGCTTTGGCCTGTTGAAGAACCTGTCAGA

AGAACAAATCGCGGTCTACAGTGCCCTGATCCAGGACGGGATGAAGGTTATTCTCATTGAGCTGGAGAAA

ATCGAGGACTACACAGTCATGCCAGAGTCAATTCAGTACATCAAACAGAAGCATGGTGCCATCCGGTGGC

ATGGGGACTTCACGGAGCAGTCACAGTGTATGAAGACCAAGTTTTGGAAGACAGTGAGATACCACATGCC

GCCCAGAAGGTGTCGGCCGTTTCCTCCGGTCCAGCTGCTGCAGCACACACCTTGCTACCGCACCGCAGGC

CCAGAACTAGGCTCAAGAAGAAAGAAGTGTACTCTCACGACTGGCTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Genomics 67: 284 (2000).

2. Circulation 106:2961 (2002). 

3. Nat. Immunol. 5:373 (2004). 

4. Proc. Natl. Acad. Sci. 105:2723 (2008). 

5. J. Exp. Med. 205:339 (2008). 

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.