Human IL1R1/IL1RA/CD121a Gene, Full-length cDNA in Lentivector, Endotoxin-free DNA

Product ID: pLTC-IL1R1 (SKU#: LTP1312)

Price:

$1,096.00

Description

IL1R1 (interleukin 1 receptor, type I), also known as CD121a, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin 1 receptor (IL1R) family. The IL1R family comprises at least 10 members, which typically contain 3 immunoglobulin (Ig)-like domains in the extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. There are two distinct types of receptors for the pleiotropic cytokines IL1α and IL1β: the type I (IL1R1) is an 80 kDa transmembrane protein expressed predominantly in T cells, fibroblasts, and endothelial cells and mediates all the known IL-1 biological functions while the type II (IL1R2) is a 68 kDa transmembrane protein with a short cytoplamic domain and serves as a decoy receptor for IL1. Both receptors are members of the immunoglobulin superfamily (IgSF). However the two receptors do not heterodimerize into a receptor complex. IL1R1 acts as a receptor for both IL-1α and IL-1β through the association with the co-receptor IL1RAP (interleukin 1 receptor antagonist protein). IL1R1 and IL1RAP together form a high affinity IL-1 receptor complex, which mediates IL-1-dependent activation of NF-κB, MAPK and other signaling pathways. IL-1R1 signaling involves the recruitment of adapter molecules, such as MYD88 and IRAK1/IRAK2, via the respective TIR domains. IL1R1 is an important mediator involved in many cytokine induced immune and inflammatory responses. Recombinant soluble form of IL1R1 has been shown to be a potent antagonist of IL1 action.

Product Details

Gene Symbol: IL1R1; CD121a; P80; IL1R; IL1RA; IL1RT1; D2S1473; IL-1R-α; IL-1R-1; IL-1RT-1; IL-1R1

NCBI Gene ID: 3554

Uniprot Entry: P14778

Construct Details: Full length human IL1R1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC or pLTC-Marker (see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 1710 (bp)

Gene Insert Sequence:

ATGAAAGTGTTACTCAGACTTATTTGTTTCATAGCTCTACTGATTTCTTCTCTGGAGGCTGATAAATGCAAGGAACGTGA

AGAAAAAATAATTTTAGTGTCATCTGCAAATGAAATTGATGTTCGTCCCTGTCCTCTTAACCCAAATGAACACAAAGGCA

CTATAACTTGGTATAAAGATGACAGCAAGACACCTGTATCTACAGAACAAGCCTCCAGGATTCATCAACACAAAGAGAAA

CTTTGGTTTGTTCCTGCTAAGGTGGAGGATTCAGGACATTACTATTGCGTGGTAAGAAATTCATCTTACTGCCTCAGAAT

TAAAATAAGTGCAAAATTTGTGGAGAATGAGCCTAACTTATGTTATAATGCACAAGCCATATTTAAGCAGAAACTACCCG

TTGCAGGAGACGGAGGACTTGTGTGCCCTTATATGGAGTTTTTTAAAAATGAAAATAATGAGTTACCTAAATTACAGTGG

TATAAGGATTGCAAACCTCTACTTCTTGACAATATACACTTTAGTGGAGTCAAAGATAGGCTCATCGTGATGAATGTGGC

TGAAAAGCATAGAGGGAACTATACTTGTCATGCATCCTACACATACTTGGGCAAGCAATATCCTATTACCCGGGTAATAG

AATTTATTACTCTAGAGGAAAACAAACCCACAAGGCCTGTGATTGTGAGCCCAGCTAATGAGACAATGGAAGTAGACTTG

GGATCCCAGATACAATTGATCTGTAATGTCACCGGCCAGTTGAGTGACATTGCTTACTGGAAGTGGAATGGGTCAGTAAT

TGATGAAGATGACCCAGTGCTAGGGGAAGACTATTACAGTGTGGAAAATCCTGCAAACAAAAGAAGGAGTACCCTCATCA

CAGTGCTTAATATATCGGAAATTGAAAGTAGATTTTATAAACATCCATTTACCTGTTTTGCCAAGAATACACATGGTATA

GATGCAGCATATATCCAGTTAATATATCCAGTCACTAATTTCCAGAAGCACATGATTGGTATATGTGTCACGTTGACAGT

CATAATTGTGTGTTCTGTTTTCATCTATAAAATCTTCAAGATTGACATTGTGCTTTGGTACAGGGATTCCTGCTATGATT

TTCTCCCAATAAAAGCTTCAGATGGAAAGACCTATGACGCATATATACTGTATCCAAAGACTGTTGGGGAAGGGTCTACC

TCTGACTGTGATATTTTTGTGTTTAAAGTCTTGCCTGAGGTCTTGGAAAAACAGTGTGGATATAAGCTGTTCATTTATGG

AAGGGATGACTACGTTGGGGAAGACATTGTTGAGGTCATTAATGAAAACGTAAAGAAAAGCAGAAGACTGATTATCATTT

TAGTCAGAGAAACATCAGGCTTCAGCTGGCTGGGTGGTTCATCTGAAGAGCAAATAGCCATGTATAATGCTCTTGTTCAG

GATGGAATTAAAGTTGTCCTGCTTGAGCTGGAGAAAATCCAAGACTATGAGAAAATGCCAGAATCGATTAAATTCATTAA

GCAGAAACATGGGGCTATCCGCTGGTCAGGGGACTTTACACAGGGACCACAGTCTGCAAAGACAAGGTTCTGGAAGAATG

TCAGGTACCACATGCCAGTCCAGCGACGGTCACCTTCATCTAAACACCAGTTACTGTCACCAGCCACTAAGGAGAAACTG

CAAAGAGAGGCTCACGTGCCTCTCGGGTAG

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References