IL18RAP (interleukin-18 receptor accessory protein), also known as IL1R7, IL18Rbeta and CD218b, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin-1 receptor (IL1R) family. Unlike other family members, IL18RAP contain only 2 immunoglobulin (Ig)-like domains in the extracellular region and an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL18RAP is an accessory subunit of the heterodimeric receptor for IL18, a proinflammatory cytokine involved in inducing cell-mediated immunity. IL18RAP enhances the IL18-binding activity of the IL18 receptor and plays a role in signaling by IL18. Within the IL18 receptor complex, IL18RAP does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation. Mutations in the IL18RAP gene are associated with Crohn's disease and inflammatory bowel disease, and susceptibility to celiac disease and leprosy.

IL18RAP (interleukin-18 receptor accessory protein), also known as IL1R7, IL18Rbeta and CD218b, is a single-pass, type I transmembrane glycoprotein that belongs to the interleukin-1 receptor (IL1R) family. Unlike other family members, IL18RAP contain only 2 immunoglobulin (Ig)-like domains in the extracellular region and an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. IL18RAP is an accessory subunit of the heterodimeric receptor for IL18, a proinflammatory cytokine involved in inducing cell-mediated immunity. IL18RAP enhances the IL18-binding activity of the IL18 receptor and plays a role in signaling by IL18. Within the IL18 receptor complex, IL18RAP does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation. Mutations in the IL18RAP gene are associated with Crohn's disease and inflammatory bowel disease, and susceptibility to celiac disease and leprosy.

 

Product Details

 

Gene Symbol: IL18RAP; ACPL; CD218b; IL1R7; IL18RB; CDw218b; IL-1R-7; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta

 

NCBI Gene ID: 8807

 

Uniprot Entry: Q95256

 

Construct Details: Full length human IL18RAP gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter -/+ an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1800 (bp)

 

Gene Insert Sequence:  

ATGCTCTGTTTGGGCTGGATATTTCTTTGGCTTGTTGCAGGAGAGCGAATTAAAGGATTTAATATTTCAG

GTTGTTCCACAAAAAAACTCCTTTGGACATATTCTACAAGGAGTGAAGAGGAATTTGTCTTATTTTGTGA

TTTACCAGAGCCACAGAAATCACATTTCTGCCACAGAAATCGACTCTCACCAAAACAAGTCCCTGAGCAC

CTGCCCTTCATGGGTAGTAACGACCTATCTGATGTCCAATGGTACCAACAACCTTCGAATGGAGATCCAT

TAGAGGACATTAGGAAAAGCTATCCTCACATCATTCAGGACAAATGTACCCTTCACTTTTTGACCCCAGG

GGTGAATAATTCTGGGTCATATATTTGTAGACCCAAGATGATTAAGAGCCCCTATGATGTAGCCTGTTGT

GTCAAGATGATTTTAGAAGTTAAGCCCCAGACAAATGCATCCTGTGAGTATTCCGCATCACATAAGCAAG

ACCTACTTCTTGGGAGCACTGGCTCTATTTCTTGCCCCAGTCTCAGCTGCCAAAGTGATGCACAAAGTCC

AGCGGTAACCTGGTACAAGAATGGAAAACTCCTCTCTGTGGAAAGGAGCAACCGAATCGTAGTGGATGAA

GTTTATGACTATCACCAGGGCACATATGTATGTGATTACACTCAGTCGGATACTGTGAGTTCGTGGACAG

TCAGAGCTGTTGTTCAAGTGAGAACCATTGTGGGAGACACTAAACTCAAACCAGATATTCTGGATCCTGT

CGAGGACACACTGGAAGTAGAACTTGGAAAGCCTTTAACTATTAGCTGCAAAGCACGATTTGGCTTTGAA

AGGGTCTTTAACCCTGTCATAAAATGGTACATCAAAGATTCTGACCTAGAGTGGGAAGTCTCAGTACCTG

AGGCGAAAAGTATTAAATCCACTTTAAAGGATGAAATCATTGAGCGTAATATCATCTTGGAAAAAGTCAC

TCAGCGTGATCTTCGCAGGAAGTTTGTTTGCTTTGTCCAGAACTCCATTGGAAACACAACCCAGTCCGTC

CAACTGAAAGAAAAGAGAGGAGTGGTGCTCCTGTACATCCTGCTTGGCACCATCGGGACCCTGGTGGCCG

TGCTGGCGGCGAGTGCCCTCCTCTACAGGCACTGGATTGAAATAGTGCTGCTGTACCGGACCTACCAGAG

CAAGGATCAGACGCTTGGGGATAAAAAGGATTTTGATGCTTTCGTATCCTATGCAAAATGGAGCTCTTTT

CCAAGTGAGGCCACTTCATCTCTGAGTGAAGAACACTTGGCCCTGAGCCTATTTCCTGATGTTTTAGAAA

ACAAATATGGATATAGCCTGTGTTTGCTTGAAAGAGATGTGGCTCCAGGAGGAGTGTATGCAGAAGACAT

TGTGAGCATTATTAAGAGAAGCAGAAGAGGAATATTTATCTTGAGCCCCAACTATGTCAATGGACCCAGT

ATCTTTGAACTACAAGCAGCAGTGAATCTTGCCTTGGATGATCAAACACTGAAACTCATTTTAATTAAGT

TCTGTTACTTCCAAGAGCCAGAGTCTCTACCTCATCTCGTGAAAAAAGCTCTCAGGGTTTTGCCCACAGT

TACTTGGAGAGGCTTAAAATCAGTTCCTCCCAATTCTAGGTTCTGGGCCAAAATGCGCTACCACATGCCT

GTGAAAAACTCTCAGGGATTCACGTGGAACCAGCTCAGAATTACCTCTAGGATTTTTCAGTGGAAAGGAC

TCAGTAGAACAGAAACCACTGGGAGGAGCTCCCAGCCTAAGGAATGGTGA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.