TLR3 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucine­rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL­1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR3 contains 22 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes double-stranded RNA (dsRNA) associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. TLR3 may thus play a role in host defense against viruses. 

TLR3 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucine­rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL­1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR3 contains 22 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes double-stranded RNA (dsRNA) associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. TLR3 may thus play a role in host defense against viruses. 

 

Product Details

 

Gene Symbol: TLR3; CD283; IIAE2

 

NCBI Gene ID: 7098

 

Uniprot Entry: O60603

 

Construct Details: Full length human TLR3/CD283 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter +/- an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-Marker (lentiviral expression vector containing a heterologous CMV promoter +/- selection marker, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGAGACAGACTTTGCCTTGTATCTACTTTTGGGGGGGCCTTTTGCCCTTTGGGATGCTGTGTGCATCCT

CCACCACCAAGTGCACTGTTAGCCATGAAGTTGCTGACTGCAGCCACCTGAAGTTGACTCAGGTACCCGA

TGATCTACCCACAAACATAACAGTGTTGAACCTTACCCATAATCAACTCAGAAGATTACCAGCCGCCAAC

TTCACAAGGTATAGCCAGCTAACTAGCTTGGATGTAGGATTTAACACCATCTCAAAACTGGAGCCAGAAT

TGTGCCAGAAACTTCCCATGTTAAAAGTTTTGAACCTCCAGCACAATGAGCTATCTCAACTTTCTGATAA

AACCTTTGCCTTCTGCACGAATTTGACTGAACTCCATCTCATGTCCAACTCAATCCAGAAAATTAAAAAT

AATCCCTTTGTCAAGCAGAAGAATTTAATCACATTAGATCTGTCTCATAATGGCTTGTCATCTACAAAAT

TAGGAACTCAGGTTCAGCTGGAAAATCTCCAAGAGCTTCTATTATCAAACAATAAAATTCAAGCGCTAAA

AAGTGAAGAACTGGATATCTTTGCCAATTCATCTTTAAAAAAATTAGAGTTGTCATCGAATCAAATTAAA

GAGTTTTCTCCAGGGTGTTTTCACGCAATTGGAAGATTATTTGGCCTCTTTCTGAACAATGTCCAGCTGG

GTCCCAGCCTTACAGAGAAGCTATGTTTGGAATTAGCAAACACAAGCATTCGGAATCTGTCTCTGAGTAA

CAGCCAGCTGTCCACCACCAGCAATACAACTTTCTTGGGACTAAAGTGGACAAATCTCACTATGCTCGAT

CTTTCCTACAACAACTTAAATGTGGTTGGTAACGATTCCTTTGCTTGGCTTCCACAACTAGAATATTTCT

TCCTAGAGTATAATAATATACAGCATTTGTTTTCTCACTCTTTGCACGGGCTTTTCAATGTGAGGTACCT

GAATTTGAAACGGTCTTTTACTAAACAAAGTATTTCCCTTGCCTCACTCCCCAAGATTGATGATTTTTCT

TTTCAGTGGCTAAAATGTTTGGAGCACCTTAACATGGAAGATAATGATATTCCAGGCATAAAAAGCAATA

TGTTCACAGGATTGATAAACCTGAAATACTTAAGTCTATCCAACTCCTTTACAAGTTTGCGAACTTTGAC

AAATGAAACATTTGTATCACTTGCTCATTCTCCCTTACACATACTCAACCTAACCAAGAATAAAATCTCA

AAAATAGAGAGTGATGCTTTCTCTTGGTTGGGCCACCTAGAAGTACTTGACCTGGGCCTTAATGAAATTG

GGCAAGAACTCACAGGCCAGGAATGGAGAGGTCTAGAAAATATTTTCGAAATCTATCTTTCCTACAACAA

GTACCTGCAGCTGACTAGGAACTCCTTTGCCTTGGTCCCAAGCCTTCAACGACTGATGCTCCGAAGGGTG

GCCCTTAAAAATGTGGATAGCTCTCCTTCACCATTCCAGCCTCTTCGTAACTTGACCATTCTGGATCTAA

GCAACAACAACATAGCCAACATAAATGATGACATGTTGGAGGGTCTTGAGAAACTAGAAATTCTCGATTT

GCAGCATAACAACTTAGCACGGCTCTGGAAACACGCAAACCCTGGTGGTCCCATTTATTTCCTAAAGGGT

CTGTCTCACCTCCACATCCTTAACTTGGAGTCCAACGGCTTTGACGAGATCCCAGTTGAGGTCTTCAAGG

ATTTATTTGAACTAAAGATCATCGATTTAGGATTGAATAATTTAAACACACTTCCAGCATCTGTCTTTAA

TAATCAGGTGTCTCTAAAGTCATTGAACCTTCAGAAGAATCTCATAACATCCGTTGAGAAGAAGGTTTTC

GGGCCAGCTTTCAGGAACCTGACTGAGTTAGATATGCGCTTTAATCCCTTTGATTGCACGTGTGAAAGTA

TTGCCTGGTTTGTTAATTGGATTAACGAGACCCATACCAACATCCCTGAGCTGTCAAGCCACTACCTTTG

CAACACTCCACCTCACTATCATGGGTTCCCAGTGAGACTTTTTGATACATCATCTTGCAAAGACAGTGCC

CCCTTTGAACTCTTTTTCATGATCAATACCAGTATCCTGTTGATTTTTATCTTTATTGTACTTCTCATCC

ACTTTGAGGGCTGGAGGATATCTTTTTATTGGAATGTTTCAGTACATCGAGTTCTTGGTTTCAAAGAAAT

AGACAGACAGACAGAACAGTTTGAATATGCAGCATATATAATTCATGCCTATAAAGATAAGGATTGGGTC

TGGGAACATTTCTCTTCAATGGAAAAGGAAGACCAATCTCTCAAATTTTGTCTGGAAGAAAGGGACTTTG

AGGCGGGTGTTTTTGAACTAGAAGCAATTGTTAACAGCATCAAAAGAAGCAGAAAAATTATTTTTGTTAT

AACACACCATCTATTAAAAGACCCATTATGCAAAAGATTCAAGGTACATCATGCAGTTCAACAAGCTATT

GAACAAAATCTGGATTCCATTATATTGGTTTTCCTTGAGGAGATTCCAGATTATAAACTGAACCATGCAC

TCTGTTTGCGAAGAGGAATGTTTAAATCTCACTGCATCTTGAACTGGCCAGTTCAGAAAGAACGGATAGG

TGCCTTTCGTCATAAATTGCAAGTAGCACTTGGATCCAAAAACTCTGTACATTAA

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.