TLR1 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucine­rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL­1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR1 contains 19 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR1 is ubiquitously expressed, and at higher levels than other TLRs. It is found on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand­independent association with TLR2.  TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols.  

TLR1 is a single-pass type I transmembrane glycoprotein belonging to the Toll-like receptor (TLR) family. The TLR family members play a fundamental role in pathogen recognition and activation of innate immunity. They serve as pattern recognition receptors for microbial pathogens. There are at least 11 mouse and 10 human TLR members. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs contain a large number of leucine­rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL­1 receptor (TIR) domain. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR1 contains 19 LRR repeats and 1 LRRCT in the extracellular region. The various TLRs exhibit different patterns of expression. TLR1 is ubiquitously expressed, and at higher levels than other TLRs. It is found on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand­independent association with TLR2.  TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols.  

 

Product Details

 

Gene Symbol: TLR1; TIL; CD281; rsc786; TIL. LPRS5; KIAA0012

 

NCBI Gene ID: 7096

 

Uniprot Entry: Q15399

 

Construct Details: The recombinant human TLR1-Fc fusion protein  is expressed as a 778 amino acid protein consisting of Ser25 ­ Glu574 region of TLR1 (UniProt accession #Q15399) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image above).  

 

Source: Human cells stably expressing human TLR1-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence:

 

        10         20         30         40         50         60 

SEFLVDRSKN GLIHVPKDLS QKTTILNISQ NYISELWTSD ILSLSKLRIL IISHNRIQYL 

        70         80         90        100        110        120 

DISVFKFNQE LEYLDLSHNK LVKISCHPTV NLKHLDLSFN AFDALPICKE FGNMSQLKFL 

       130        140        150        160        170        180 

 GLSTTHLEKS SVLPIAHLNI SKVLLVLGET YGEKEDPEGL QDFNTESLHI VFPTNKEFHF 

       190        200        210        220        230        240 

ILDVSVKTVA NLELSNIKCV LEDNKCSYFL SILAKLQTNP KLSNLTLNNI ETTWNSFIRI 

       250        260        270        280        290        300 

LQLVWHTTVW YFSISNVKLQ GQLDFRDFDY SGTSLKALSI HQVVSDVFGF PQSYIYEIFS  

       310        320        330        340        350        360 

NMNIKNFTVS GTRMVHMLCP SKISPFLHLD FSNNLLTDTV FENCGHLTEL ETLILQMNQL 

       370        380        390        400        410        420 

KELSKIAEMT TQMKSLQQLD ISQNSVSYDE KKGDCSWTKS LLSLNMSSNI LTDTIFRCLP 

       430        440        450        460        470        480 

PRIKVLDLHS NKIKSIPKQV VKLEALQELN VAFNSLTDLP GCGSFSSLSV LIIDHNSVSH  

       490        500        510        520        530        540 

PSADFFQSCQ KMRSIKAGDN PFQCTCELGE FVKNIDQVSS EVLEGWPDSY KCDYPESYRG 

       550        560        570        580        590        600  

TLLKDFHMSE STGTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE 

       610        620        630        640        650        660 

DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP 

       670        680        690        700        710        720 

APIEKTISKA KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN 

       730        740        750        760        770  

NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK

  

M.W.: Calculated molecular mass (kDa): 88.1; Estimated by SDS-PAGE under reducing condition (kDa): 90-100

 

Calculated PI: 6.16

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  88905

 

Purity: >90% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to the triacylated peptide biotinylated Pam3CysSerLys4 (PAM3CSK4).

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 95:588 (1998). 

2. Proc. Natl. Acad. Sci. 97:13766 (2000). 

3. J. Biol. Chem. 279:16971 (2004). 

4. J. Immunol. 174:1566 (2005).

5. J. Biol. Chem. 280:8606. 13 (2005).

6. Semin. Immunol. 19:3 (2007).

  

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL00J4-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.