CLEC4M (C-type lectin domain family 4 member C) or CD299, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC4M is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. CLEC4M is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. CLEC4M contains 1 tandem-repeat neck domain and 1 C-type lectin domain in the extracellular region with a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells.  The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity.  CLEC4M is closely related in terms of both sequence and function to a neighboring gene DC-SIGN or CD209. They differ in their ligand-binding properties and distribution. CLEC4M may act as a pathogen-recognition receptor involved in peripheral immune surveillance in liver. CLEC4M may mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. It is also a receptor for ICAM3, probably by binding to mannose-like carbohydrates.CLEC4M acts as an attachment receptor for ebola virus, hepatitis C virus, HIV-1 ,  coronavirus 229E, cytomegalovirus/HHV-5, influenzavirus, SARS coronavirus, and west-nile virus etc.

CLEC4M (C-type lectin domain family 4 member C) or CD299, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. CLEC4M is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. CLEC4M is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. CLEC4M contains 1 tandem-repeat neck domain and 1 C-type lectin domain in the extracellular region with a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells.  The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity.  CLEC4M is closely related in terms of both sequence and function to a neighboring gene DC-SIGN or CD209. They differ in their ligand-binding properties and distribution. CLEC4M may act as a pathogen-recognition receptor involved in peripheral immune surveillance in liver. CLEC4M may mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. It is also a receptor for ICAM3, probably by binding to mannose-like carbohydrates.CLEC4M acts as an attachment receptor for ebola virus, hepatitis C virus, HIV-1 ,  coronavirus 229E, cytomegalovirus/HHV-5, influenzavirus, SARS coronavirus, and west-nile virus etc.

 

Product Details

 

Gene Symbol: CLEC4M; CD299; LSIGN; CD209L; L-SIGN; DCSIGNR; HP10347; DC-SIGN2; DC-SIGNR

 

NCBI Gene ID: 10332

 

Uniprot Entry: Q9H2X3

 

Construct Details: Full length human CLEC4C/CD303 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1200 (bp)

 

Gene Insert Sequence:  

ATGAGTGACTCCAAGGAACCAAGGGTGCAGCAGCTGGGCCTCCTGGAAGAAGATCCAACAACCAGTGGCA

TCAGACTTTTTCCAAGAGACTTTCAATTCCAGCAGATACATGGCCACAAGAGCTCTACAGGGTGTCTTGG

CCATGGCGCCCTGGTGCTGCAACTCCTCTCCTTCATGCTCTTGGCTGGGGTCCTGGTGGCCATCCTTGTC

CAAGTGTCCAAGGTCCCCAGCTCCCTAAGTCAGGAACAATCCGAGCAAGACGCAATCTACCAGAACCTGA

CCCAGCTTAAAGCTGCAGTGGGTGAGCTCTCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGAC

CCAGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACC

CGGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACCC

GGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACCCG

GCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACGGAG

CTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACCCAGC

TGAAGGCTGCAGTGGGTGAGTTGCCAGACCAGTCCAAGCAGCAGCAAATCTATCAAGAACTGACCGATTT

GAAGACTGCATTTGAACGCCTGTGCCGCCACTGTCCCAAGGACTGGACATTCTTCCAAGGAAACTGTTAC

TTCATGTCTAACTCCCAGCGGAACTGGCACGACTCCGTCACCGCCTGCCAGGAAGTGAGGGCCCAGCTCG

TCGTAATCAAAACTGCTGAGGAGCAGAACTTCCTACAGCTGCAGACTTCCAGGAGTAACCGCTTCTCCTG

GATGGGACTTTCAGACCTAAATCAGGAAGGCACGTGGCAATGGGTGGACGGCTCACCTCTGTCACCCAGC

TTCCAGCGGTACTGGAACAGTGGAGAACCCAACAATAGCGGGAATGAAGACTGTGCGGAATTTAGTGGCA

GTGGCTGGAACGACAATCGATGTGACGTTGACAATTACTGGATCTGCAAAAAGCCCGCAGCCTGCTTCAG

AGACGAATAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.