CLEC4C (C-type lectin domain family 4 member C) or CD303, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily.  Members of this superfamily share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC4C contains 1 C-type lectin domain in the extracellular region and may play a role indendritic cell function.  It is expressed in immature monocyte-derived dendritic cells (iMDDC).  Its expression is significantly down-regulated upon maturation with LPS but not with TNF-alpha. CLEC4C may be involved in antigen-capturing by targeting ligand into antigen processing and peptide-loading compartments for presentation to T-cells. CLEC4C may mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. It may also act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium.

CLEC4C (C-type lectin domain family 4 member C) or CD303, is a single-pass type II transmembrane protein that belongs to a the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily.  Members of this superfamily share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC4C contains 1 C-type lectin domain in the extracellular region and may play a role indendritic cell function.  It is expressed in immature monocyte-derived dendritic cells (iMDDC).  Its expression is significantly down-regulated upon maturation with LPS but not with TNF-alpha. CLEC4C may be involved in antigen-capturing by targeting ligand into antigen processing and peptide-loading compartments for presentation to T-cells. CLEC4C may mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. It may also act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium.

 

Product Details

 

Gene Symbol: CLEC4C; DLEC; HECL; BDCA2; CD303; CLECSF7; CLECSF11; PRO34150

 

NCBI Gene ID: 170482

 

Uniprot Entry: Q8WTT0

 

Construct Details: Full length human CLEC4C/CD303 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without an antibiotic selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 642 (bp)

 

Gene Insert Sequence:  

ATGGTGCCTGAAGAAGAGCCTCAAGACCGAGAGAAAGGACTCTGGTGGTTCCAGTTGAAGGTCTGGTCCA

TGGCAGTCGTATCCATCTTGCTCCTCAGTGTCTGTTTCACTGTGAGTTCTGTGGTGCCTCACAATTTTAT

GTATAGCAAAACTGTCAAGAGGCTGTCCAAGTTACGAGAGTATCAACAGTATCATCCAAGCCTGACCTGC

GTCATGGAAGGAAAGGACATAGAAGATTGGAGCTGCTGCCCAACCCCTTGGACTTCATTTCAGTCTAGTT

GCTACTTTATTTCTACTGGGATGCAATCTTGGACTAAGAGTCAAAAGAACTGTTCTGTGATGGGGGCTGA

TCTGGTGGTGATCAACACCAGGGAAGAACAGGATTTCATCATTCAGAATCTGAAAAGAAATTCTTCTTAT

TTTCTGGGGCTGTCAGATCCAGGGGGTCGGCGACATTGGCAATGGGTTGACCAGACACCATACAATGAAA

ATGTCACATTCTGGCACTCAGGTGAACCCAATAACCTTGATGAGCGTTGTGCGATAATAAATTTCCGTTC

TTCAGAAGAATGGGGCTGGAATGACATTCACTGTCATGTACCTCAGAAGTCAATTTGCAAGATGAAGAAG

ATCTACATATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.