IL23R (interleukin-23 receptor) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL23R consists of an extracellular region with 2 fibronectin type III (FN3) domains, a transmembrane domain, and a cytoplasmic tail.   IL23R is is a subunit of the receptor complex for IL23, a heterodimer with IL12RB1 in the presence of IL23.  Both IL23R and IL12Rb1 are required for IL23 signaling. The IL23R associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.  IL23  signaling mediates T-cells, NK cells and certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Mutations in the IL23R gene are associated with inflammatory bowel disease 17 (IBD17), a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology.

IL23R (interleukin-23 receptor) is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 2 subfamily.  IL23R consists of an extracellular region with 2 fibronectin type III (FN3) domains, a transmembrane domain, and a cytoplasmic tail.   IL23R is is a subunit of the receptor complex for IL23, a heterodimer with IL12RB1 in the presence of IL23.  Both IL23R and IL12Rb1 are required for IL23 signaling. The IL23R associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.  IL23  signaling mediates T-cells, NK cells and certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Mutations in the IL23R gene are associated with inflammatory bowel disease 17 (IBD17), a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology.

 

Product Details

 

Gene Symbol: IL23R; IL-23R; IBD17

 

NCBI Gene ID: 149233

 

Uniprot Entry: Q5VWK5

 

Construct Details: Full length human IL23R gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1890 (bp)

 

Gene Insert Sequence:  

ATGAATCAGGTCACTATTCAATGGGATGCAGTAATAGCCCTTTACATACTCTTCAGCTGGTGTCATGGAG

GAATTACAAATATAAACTGCTCTGGCCACATCTGGGTAGAACCAGCCACAATTTTTAAGATGGGTATGAA

TATCTCTATATATTGCCAAGCAGCAATTAAGAACTGCCAACCAAGGAAACTTCATTTTTATAAAAATGGC

ATCAAAGAAAGATTTCAAATCACAAGGATTAATAAAACAACAGCTCGGCTTTGGTATAAAAACTTTCTGG

AACCACATGCTTCTATGTACTGCACTGCTGAATGTCCCAAACATTTTCAAGAGACACTGATATGTGGAAA

AGACATTTCTTCTGGATATCCGCCAGATATTCCTGATGAAGTAACCTGTGTCATTTATGAATATTCAGGC

AACATGACTTGCACCTGGAATGCTGGGAAGCTCACCTACATAGACACAAAATACGTGGTACATGTGAAGA

GTTTAGAGACAGAAGAAGAGCAACAGTATCTCACCTCAAGCTATATTAACATCTCCACTGATTCATTACA

AGGTGGCAAGAAGTACTTGGTTTGGGTCCAAGCAGCAAACGCACTAGGCATGGAAGAGTCAAAACAACTG

CAAATTCACCTGGATGATATAGTGATACCTTCTGCAGCCGTCATTTCCAGGGCTGAGACTATAAATGCTA

CAGTGCCCAAGACCATAATTTATTGGGATAGTCAAACAACAATTGAAAAGGTTTCCTGTGAAATGAGATA

CAAGGCTACAACAAACCAAACTTGGAATGTTAAAGAATTTGACACCAATTTTACATATGTGCAACAGTCA

GAATTCTACTTGGAGCCAAACATTAAGTACGTATTTCAAGTGAGATGTCAAGAAACAGGCAAAAGGTACT

GGCAGCCTTGGAGTTCACTGTTTTTTCATAAAACACCTGAAACAGTTCCCCAGGTCACATCAAAAGCATT

CCAACATGACACATGGAATTCTGGGCTAACAGTTGCTTCCATCTCTACAGGGCACCTTACTTCTGACAAC

AGAGGAGACATTGGACTTTTATTGGGAATGATCGTCTTTGCTGTTATGTTGTCAATTCTTTCTTTGATTG

GGATATTTAACAGATCATTCCGAACTGGGATTAAAAGAAGGATCTTATTGTTAATACCAAAGTGGCTTTA

TGAAGATATTCCTAATATGAAAAACAGCAATGTTGTGAAAATGCTACAGGAAAATAGTGAACTTATGAAT

AATAATTCCAGTGAGCAGGTCCTATATGTTGATCCCATGATTACAGAGATAAAAGAAATCTTCATCCCAG

AACACAAGCCTACAGACTACAAGAAGGAGAATACAGGACCCCTGGAGACAAGAGACTACCCGCAAAACTC

GCTATTCGACAATACTACAGTTGTATATATTCCTGATCTCAACACTGGATATAAACCCCAAATTTCAAAT

TTTCTGCCTGAGGGAAGCCATCTCAGCAATAATAATGAAATTACTTCCTTAACACTTAAACCACCAGTTG

ATTCCTTAGACTCAGGAAATAATCCCAGGTTACAAAAGCATCCTAATTTTGCTTTTTCTGTTTCAAGTGT

GAATTCACTAAGCAACACAATATTTCTTGGAGAATTAAGCCTCATATTAAATCAAGGAGAATGCAGTTCT

CCTGACATACAAAACTCAGTAGAGGAGGAAACCACCATGCTTTTGGAAAATGATTCACCCAGTGAAACTA

TTCCAGAACAGACCCTGCTTCCTGATGAATTTGTCTCCTGTTTGGGGATCGTGAATGAGGAGTTGCCATC

TATTAATACTTATTTTCCACAAAATATTTTGGAAAGCCACTTCAATAGGATTTCACTCTTGGAAAAGTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

  

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.