CRLF2 (cytokine receptor-like factor 2) is is also known as the receptor for thymic stromal lymphopoietin (TSLP). CRLF2/TSLPR is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  It consists of a extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD127/IL7R is another component of the receptor complex for TSLP. The TSLP receptor complex is a heterodimer of TSLPR/CRLF2 and CD127/IL7R, which is capable of stimulating cell proliferation through activation of STAT3, STAT5 and JAK2 pathways. CRLF2/TSLPR is implicated in the development of the hematopoietic system.  It is primarily expressed in dendrites and monocytes and weakly expressed in T-cells.  It is up-regulated in activated peripheral monocytes and THP-1 cells. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL.

CRLF2 (cytokine receptor-like factor 2) is is also known as the receptor for thymic stromal lymphopoietin (TSLP). CRLF2/TSLPR is a single pass, type I transmembrane glycoprotein of the type I cytokine receptor family, type 5 subfamily.  It consists of a extracellular region with 1 fibronectin type III (FN3) domains and a juxtamembrane WSXWS motif, a transmembrane domain, and a cytoplasmic tail with the box 1 motif.  The WSXWS motif is necessary for proper protein folding and efficient intracellular transport and cell-surface receptor binding, while the box 1 motif is required for JAK interaction/activation. CD127/IL7R is another component of the receptor complex for TSLP. The TSLP receptor complex is a heterodimer of TSLPR/CRLF2 and CD127/IL7R, which is capable of stimulating cell proliferation through activation of STAT3, STAT5 and JAK2 pathways. CRLF2/TSLPR is implicated in the development of the hematopoietic system.  It is primarily expressed in dendrites and monocytes and weakly expressed in T-cells.  It is up-regulated in activated peripheral monocytes and THP-1 cells. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL.

Product Details

 

Gene Symbol: CRLF2; CRL2; TSLPR; CRLF2Y

 

NCBI Gene ID: 64109

 

Uniprot Entry: Q9HC73

 

Construct Details: Full length human TSLPR/CRLF2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1116(bp)

 

Gene Insert Sequence:  

ATGGGGCGGCTGGTTCTGCTGTGGGGAGCTGCCGTCTTTCTGCTGGGAGGCTGGATGGCTTTGGGGCAAG

GAGGAGCAGCAGAAGGAGTACAGATTCAGATCATCTACTTCAATTTAGAAACCGTGCAGGTGACATGGAA

TGCCAGCAAATACTCCAGGACCAACCTGACTTTCCACTACAGATTCAACGGTGATGAGGCCTATGACCAG

TGCACCAACTACCTTCTCCAGGAAGGTCACACTTCGGGGTGCCTCCTAGACGCAGAGCAGCGAGACGACA

TTCTCTATTTCTCCATCAGGAATGGGACGCACCCCGTTTTCACCGCAAGTCGCTGGATGGTTTATTACCT

GAAACCCAGTTCCCCGAAGCACGTGAGATTTTCGTGGCATCAGGATGCAGTGACGGTGACGTGTTCTGAC

CTGTCCTACGGGGATCTCCTCTATGAGGTTCAGTACCGGAGCCCCTTCGACACCGAGTGGCAGTCCAAAC

AGGAAAATACCTGCAACGTCACCATAGAAGGCTTGGATGCCGAGAAGTGTTACTCTTTCTGGGTCAGGGT

GAAGGCTATGGAGGATGTATATGGGCCAGACACATACCCAAGCGACTGGTCAGAGGTGACATGCTGGCAG

AGAGGCGAGATTCGGGATGCCTGTGCAGAGACACCAACGCCTCCCAAACCAAAGCTGTCCAAATTTATTT

TAATTTCCAGCCTGGCCATCCTTCTGATGGTGTCTCTCCTCCTTCTGTCTTTATGGAAATTATGGAGAGT

GAAGAAGTTTCTCATTCCCAGCGTGCCAGACCCGAAATCCATCTTCCCCGGGCTCTTTGAGATACACCAA

GGGAACTTCCAGGAGTGGATCACAGACACCCAGAACGTGGCCCACCTCCACAAGATGGCAGGTGCAGAGC

AAGAAAGTGGCCCCGAGGAGCCCCTGGTAGTCCAGTTGGCCAAGACTGAAGCCGAGTCTCCCAGGATGCT

GGACCCACAGACCGAGGAGAAAGAGGCCTCTGGGGGATCCCTCCAGCTTCCCCACCAGCCCCTCCAAGGC

GGTGATGTGGTCACAATCGGGGGCTTCACCTTTGTGATGAATGACCGCTCCTACGTGGCGTTGTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

 

 

1. Biochem. Biophys. Res. Commun. 281:878-883(2001) 

2. Cytogenet. Cell Genet. 93:23-25(2001) 

3. J. Immunol. 167:336-343(2001)

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.