TIM­3 (T cell immunoglobulin and mucin domain­3), also known as HAVCR2, is a single-pass type I transmembrane glycoprotein of the TIM family of Ig superfamily.  Like other family members, TIM-3 contains one Ig­like V­type domain and a Ser/Thr­rich mucin stalk domain in the extracellular region. An alternatively spliced isoform is truncated within the mucin­like stalk. TIM-3 is selectively expressed on Th1 (T-helper type 1) cells but not on Th2 cells. Th1 cells produce cytokines (IFN-γ, interleukin-2, TNF-α and lymphotoxin) associated with cell-mediated immune responses against intracellular pathogens, delayed-type hypersensitivity, and induction of organ-specific autoimmunity. TIM­3 is up­regulated on activated myeloid cells and T cells. TIM-3 regulates macrophage activation and inhibits Th1-mediated auto- and allo-immune responses to promote immunological tolerance. It may be also involved in T-cell homing. Galectin­9 has been identified as a ligand for TIM-3.  The binding of Galectin-9 to TM-3 enhances immune tolerance and inhibits anti­tumor immunity. It can alternatively trigger immune stimulatory effects, such as the co-activation of NK cell cytotoxicity. TIM­3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross­presentation of apoptotic cell antigens. Dysregulation of the Galectin-9/TIM-3 pathway is implicated in many chronic autoimmune conditions in human, such as multiple sclerosis and systemic lupus erythematosus. 

TIM­3 (T cell immunoglobulin and mucin domain­3), also known as HAVCR2, is a single-pass type I transmembrane glycoprotein of the TIM family of Ig superfamily.  Like other family members, TIM-3 contains one Ig­like V­type domain and a Ser/Thr­rich mucin stalk domain in the extracellular region. An alternatively spliced isoform is truncated within the mucin­like stalk. TIM-3 is selectively expressed on Th1 (T-helper type 1) cells but not on Th2 cells. Th1 cells produce cytokines (IFN-γ, interleukin-2, TNF-α and lymphotoxin) associated with cell-mediated immune responses against intracellular pathogens, delayed-type hypersensitivity, and induction of organ-specific autoimmunity. TIM­3 is up­regulated on activated myeloid cells and T cells. TIM-3 regulates macrophage activation and inhibits Th1-mediated auto- and allo-immune responses to promote immunological tolerance. It may be also involved in T-cell homing. Galectin­9 has been identified as a ligand for TIM-3.  The binding of Galectin-9 to TM-3 enhances immune tolerance and inhibits anti­tumor immunity. It can alternatively trigger immune stimulatory effects, such as the co-activation of NK cell cytotoxicity. TIM­3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross­presentation of apoptotic cell antigens. Dysregulation of the Galectin-9/TIM-3 pathway is implicated in many chronic autoimmune conditions in human, such as multiple sclerosis and systemic lupus erythematosus. 

 

Product Details

 

Gene Symbol: TIM-3; TIM3; TIMD3; TIMD-3; KIM-3; HAVCR2; HAVCR-2

 

NCBI Gene ID: 84868

 

Uniprot Entry: Q8TDQ0

 

Construct Details: Full length human TIM-3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 906 (bp)

 

Gene Insert Sequence:  

ATGTTTTCACATCTTCCCTTTGACTGTGTCCTGCTGCTGCTGCTGCTACTACTTACAAGGTCCTCAGAAGTGGAATACAG

AGCGGAGGTCGGTCAGAATGCCTATCTGCCCTGCTTCTACACCCCAGCCGCCCCAGGGAACCTCGTGCCCGTCTGCTGGG

GCAAAGGAGCCTGTCCTGTGTTTGAATGTGGCAACGTGGTGCTCAGGACTGATGAAAGGGATGTGAATTATTGGACATCC

AGATACTGGCTAAATGGGGATTTCCGCAAAGGAGATGTGTCCCTGACCATAGAGAATGTGACTCTAGCAGACAGTGGGAT

CTACTGCTGCCGGATCCAAATCCCAGGCATAATGAATGATGAAAAATTTAACCTGAAGTTGGTCATCAAACCAGCCAAGG

TCACCCCTGCACCGACTCGGCAGAGAGACTTCACTGCAGCCTTTCCAAGGATGCTTACCACCAGGGGACATGGCCCAGCA

GAGACACAGACACTGGGGAGCCTCCCTGATATAAATCTAACACAAATATCCACATTGGCCAATGAGTTACGGGACTCTAG

ATTGGCCAATGACTTACGGGACTCTGGAGCAACCATCAGAATAGGCATCTACATCGGAGCAGGGATCTGTGCTGGGCTGG

CTCTGGCTCTTATCTTCGGCGCTTTAATTTTCAAATGGTATTCTCATAGCAAAGAGAAGATACAGAATTTAAGCCTCATC

TCTTTGGCCAACCTCCCTCCCTCAGGATTGGCAAATGCAGTAGCAGAGGGAATTCGCTCAGAAGAAAACATCTATACCAT

TGAAGAGAACGTATATGAAGTGGAGGAGCCCAATGAGTATTATTGCTATGTCAGCAGCAGGCAGCAACCCTCACAACCTT

TGGGTTGTCGCTTTGCAATGCCATAG

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Nature 415:536 (2002). 

2. Nat. Immunol. 4:1093 (2003). 

3. Nat. Immunol. 4:1102 (2003).

4. J. Exp. Med. 203:1413 (2006).

5. Science 318:1141 (2007).

 

 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP0760-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.