CD24 or CD24A is a mucin-type glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of B cells, granulocytes, epithelial, neuronal, and muscle cells, and on a range of tumor cells.  The precursor of CD24 is cleaved to a short 32 amino acid mature peptide that is anchored to the cell surface by GPI. CD24 modulates B-cell activation responses. CD24 promotes antigen-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T cells, which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 may be a genetic modifier for risk and progression of multiple sclerosis. CD24 serve as a receptor for P-selectin.  CD24 is involved in molecular adhesion and metastatic tumor spread by facilitating the interaction with platelet and endothelial cells. CD24 is considered as a tumor marker and high expressions can be detected in epithelial ovarian cancer, breast cancer, non-small cell lung cancer, prostate cancer and pancreatic cancer. 

CD24 or CD24A is a mucin-type glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of B cells, granulocytes, epithelial, neuronal, and muscle cells, and on a range of tumor cells.  The precursor of CD24 is cleaved to a short 32 amino acid mature peptide that is anchored to the cell surface by GPI. CD24 modulates B-cell activation responses. CD24 promotes antigen-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T cells, which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 may be a genetic modifier for risk and progression of multiple sclerosis. CD24 serve as a receptor for P-selectin.  CD24 is involved in molecular adhesion and metastatic tumor spread by facilitating the interaction with platelet and endothelial cells. CD24 is considered as a tumor marker and high expressions can be detected in epithelial ovarian cancer, breast cancer, non-small cell lung cancer, prostate cancer and pancreatic cancer. 

 

Product Details

 

Gene Symbol: CD24; CD24A

 

NCBI Gene ID: 100133941

 

Uniprot Entry: P25063

 

Construct Details: The recombinant human CD24-Fc fusion is expressed as a 260 amino acid protein consisting of Ser27 - Ala58 region of CD24 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

  

Source: Human cells stably expressing human CD24-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

SETTTGTSSN SSQSTSNSGL APNPTNATTK AAVDKTHTCP PCPAPELLGG PSVFLFPPKP 

        70         80         90        100        110        120 

KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT 

       130        140        150        160        170        180 

VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC 

       190        200        210        220        230        240 

LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV 

       250        260 

MHEALHNHYT QKSLSLSPGK 

  

M.W.: Calculated molecular mass (kDa): 29; Estimated by SDS-PAGE under reducing condition (kDa): ~45

 

Calculated PI: 7.02

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 35785

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above)

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method 

 

Bioactivity:  Binds to human P-selectin and Siglec-10 proteins by ELISA

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Immunol. 147:1412-1416(1991)

2. Cancer Res. 52:5264-5270(1992)

3. J. Immunol. 166:5567-5577(2001) 

4. Proc. Natl. Acad. Sci. 100:15041-15046(2003) 

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.