CD6, also known as TP120, is a single-pass type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 contains three SRCR domains in the extracellular region. CD6 is a cell surface glycoprotein expressed primarily on T cells and thus a T-cell differentiation antigen. CD6 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some neuronal cells in the brain.  CD6 may function as both a co-stimulatory molecule in T cell activation and as an adhesion receptor.  As a cell adhesion molecule, CD6 mediates cell-cell contacts and regulates T cell responses via its interaction with the ligand ALCAM (activated leukocyte cell adhesion molecule) or CD166. CD6 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse on T lymphocytes. CD6/ALCAM interactions are thought to play a role in thymocyte development.  CD6 may also contribute to signaling cascades triggered by activation of the TCR/CD3 complex and the overall functional avidity of selection. CD6 also functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. CD6 binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. CD6 thus mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 may be associated with susceptibility to multiple sclerosis

CD6, also known as TP120, is a single-pass type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 contains three SRCR domains in the extracellular region. CD6 is a cell surface glycoprotein expressed primarily on T cells and thus a T-cell differentiation antigen. CD6 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some neuronal cells in the brain.  CD6 may function as both a co-stimulatory molecule in T cell activation and as an adhesion receptor.  As a cell adhesion molecule, CD6 mediates cell-cell contacts and regulates T cell responses via its interaction with the ligand ALCAM (activated leukocyte cell adhesion molecule) or CD166. CD6 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse on T lymphocytes. CD6/ALCAM interactions are thought to play a role in thymocyte development.  CD6 may also contribute to signaling cascades triggered by activation of the TCR/CD3 complex and the overall functional avidity of selection. CD6 also functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. CD6 binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. CD6 thus mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS. CD6 may be associated with susceptibility to multiple sclerosis

 

Product Details

 

Gene Symbol: CD6; TP120; T12

 

NCBI Gene ID: 923

 

Uniprot Entry: P30203

 

Construct Details: Full length human CD6 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Sequence:  

ATGTGGCTCTTCTTCGGGATCACTGGATTGCTGACGGCAGCCCTCTCAGGTCATCCATCTCCAGCCCCAC

CTGACCAGCTCAACACCAGCAGTGCAGAGAGTGAGCTCTGGGAGCCAGGGGAGCGGCTTCCGGTCCGTCT

GACAAACGGGAGCAGCAGCTGCAGCGGGACGGTGGAGGTGCGGCTCGAGGCGTCCTGGGAGCCCGCGTGC

GGGGCGCTCTGGGACAGCCGCGCCGCCGAGGCCGTGTGCCGAGCACTGGGCTGCGGCGGGGCGGAGGCCG

CCTCTCAGCTCGCCCCGCCGACCCCTGAGCTGCCGCCCCCGCCTGCAGCCGGGAACACCAGCGTAGCAGC

TAATGCCACTCTGGCCGGGGCGCCCGCCCTCCTGTGCAGCGGCGCCGAGTGGCGGCTCTGCGAGGTGGTG

GAGCACGCGTGCCGCAGCGACGGGAGGCGGGCCCGTGTCACCTGTGCAGAGAACCGCGCGCTGCGCCTGG

TGGACGGTGGCGGCGCCTGCGCCGGCCGCGTGGAGATGCTGGAGCATGGCGAGTGGGGATCAGTGTGCGA

TGACACTTGGGACCTGGAGGACGCCCACGTGGTGTGCAGGCAACTGGGCTGCGGCTGGGCAGTCCAGGCC

CTGCCCGGCTTGCACTTCACGCCCGGCCGCGGGCCTATCCACCGGGACCAGGTGAACTGCTCGGGGGCCG

AAGCTTACCTGTGGGACTGCCCGGGGCTGCCAGGACAGCACTACTGCGGCCACAAAGAGGACGCGGGCGC

GGTGTGCTCAGAGCACCAGTCCTGGCGCCTGACAGGGGGCGCTGACCGCTGCGAGGGGCAGGTGGAGGTA

CACTTCCGAGGGGTCTGGAACACAGTGTGTGACAGTGAGTGGTACCCATCGGAGGCCAAGGTGCTCTGCC

AGTCCTTGGGCTGTGGAACTGCGGTTGAGAGGCCCAAGGGGCTGCCCCACTCCTTGTCCGGCAGGATGTA

CTACTCATGCAATGGGGAGGAGCTCACCCTCTCCAACTGCTCCTGGCGGTTCAACAACTCCAACCTCTGC

AGCCAGTCGCTGGCAGCCAGGGTCCTCTGCTCAGCTTCCCGGAGTTTGCACAATCTGTCCACTCCCGAAG

TCCCTGCAAGTGTTCAGACAGTCACTATAGAATCTTCTGTGACAGTGAAAATAGAGAACAAGGAATCTCG

GGAGCTAATGCTCCTCATCCCCTCCATCGTTCTGGGAATTCTCCTCCTTGGCTCCCTCATCTTCATAGCC

TTCATCCTCTTGAGAATTAAAGGAAAATATGCCCTCCCCGTAATGGTGAACCACCAGCACCTACCCACCA

CCATCCCGGCAGGGAGCAATAGCTATCAACCGGTCCCCATCACCATCCCCAAAGAAGTTTTCATGCTGCC

CATCCAGGTCCAGGCCCCGCCCCCTGAGGACTCAGACTCTGGCTCGGACTCAGACTATGAGCACTATGAC

TTCAGCGCCCAGCCTCCTGTGGCCCTGACCACCTTCTACAATTCCCAGCGGCATCGGGTCACAGATGAGG

AGGTCCAGCAAAGCAGGTTCCAGATGCCACCCTTGGAGGAAGGACTTGAAGAGTTGCATGCCTCCCACAT

CCCAACTGCCAACCCTGGACACTGCATTACAGACCCGCCATCCCTGGGCCCTCAGTATCACCCGAGGAGC

AACAGTGAGTCGAGCACCTCTTCAGGGGAGGATTACTGCAATAGTCCCAAAAGCAAGCTGCCTCCATGGA

ACCCCCAGGTGTTTTCTTCAGAGAGGAGTTCCTTCCTGGAGCAGCCCCCAAACTTGGAGCTGGCCGGCAC

CCAGCCAGCCTTTTCAGCAGGGCCCCCGGCTGATGACAGCTCCAGCACCTCATCCGGGGAGTGGTACCAG

AACTTCCAGCCACCACCCCAGCCCCCTTCGGAGGAGCAGTTTGGCTGTCCAGGGTCCCCCAGCCCTCAGC

CTGACTCCACCGACAACGATGACTACGATGACATCAGCGCAGCCTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Immunol. 143:2439 (1989)

2. J. Exp. Med. 174:949 (1991)

3. Eur. J. Immunol. 25:2765 (1995)

4. J. Immunol. 158:1149 (1997)

5. J. Immunol. 178:4351 (2007)

6. Proc. Natl. Acad. Sci. 104:11724 (2007)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2579-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.