B-cell differentiation antigen CD72, also known as Lyb2, is a single pass type II transmembrane glycoprotein that plays an important role in adaptive immunity. CD72 contains a 95 amino acid (aa) cytoplasmic domain with 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain (ECD) with a coiled-coil domain and a C-type lectin domain. CD72 is expressed on B lineage cells including Pre-B-cells and B-cells but not terminally differentiated plasma cells, NK cells, monocytes, dendritic cells, and mast cells. CD5 exists as a disulfide-linked homodimer. It interacts with CD5 and with CD100/Semaphorin 4D.  CD5 also associates with CD79a in the B cell antigen receptor (BCR) complex following antigen stimulation.  CD5 inhibits BCR signaling through interactions with the phosphatase PTPN6/SHP-1. CD72 ligation with antibodies or with CD100 induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP-1, leading to B cell proliferation. CD100 is expressed on activated NK cells and the adhesive interaction between CD100+ NK cells and CD72+ cells leads to an enhanced killing and IFN­γ secretion.  Both CD72 and CD100 are required for the maintenance of B cell anergy and tolerance.  Mice that lack either CD72 or CD100 develop autoimmunity.  CD72 may also induce positive signaling in B cells independent of the BCR signaling. 

B-cell differentiation antigen CD72, also known as Lyb2, is a single pass type II transmembrane glycoprotein that plays an important role in adaptive immunity. CD72 contains a 95 amino acid (aa) cytoplasmic domain with 2 immunoreceptor tyrosine-based inhibitory motifs (ITIMs), a 21 aa transmembrane segment, and a 243 aa extracellular domain (ECD) with a coiled-coil domain and a C-type lectin domain. CD72 is expressed on B lineage cells including Pre-B-cells and B-cells but not terminally differentiated plasma cells, NK cells, monocytes, dendritic cells, and mast cells. CD5 exists as a disulfide-linked homodimer. It interacts with CD5 and with CD100/Semaphorin 4D.  CD5 also associates with CD79a in the B cell antigen receptor (BCR) complex following antigen stimulation.  CD5 inhibits BCR signaling through interactions with the phosphatase PTPN6/SHP-1. CD72 ligation with antibodies or with CD100 induces tyrosine dephosphorylation of the CD72 cytoplasmic domain and its dissociation from SHP-1, leading to B cell proliferation. CD100 is expressed on activated NK cells and the adhesive interaction between CD100+ NK cells and CD72+ cells leads to an enhanced killing and IFN­γ secretion.  Both CD72 and CD100 are required for the maintenance of B cell anergy and tolerance.  Mice that lack either CD72 or CD100 develop autoimmunity.  CD72 may also induce positive signaling in B cells independent of the BCR signaling. 

 

Product Details

 

Gene Symbol: CD72; LYB2; CD72b; Lyb-2

 

NCBI Gene ID: 971

 

Uniprot Entry: P21854

 

Construct Details: Full length human CD72 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1080 (bp)

 

Gene Insert Sequence:  

ATGGCTGAGGCCATCACCTATGCAGATCTGAGGTTTGTGAAGGCTCCCCTGAAGAAGAGCATCTCCAGCCGGTTAGGACA

GGACCCAGGGGCTGATGATGATGGGGAAATCACCTACGAGAATGTTCAAGTGCCCGCAGTCCTAGGGGTGCCCTCAAGCT

TGGCTTCTTCTGTACTAGGGGACAAAGCAGCGGTCAAGTCGGAGCAGCCAACTGCGTCCTGGAGAGCCGTGACGTCACCA

GCTGTCGGGCGGATTCTCCCCTGCCGCACAACCTGCCTGCGATACCTCCTGCTCGGCCTGCTCCTCACCTGCCTGCTGTT

AGGAGTGACCGCCATCTGCCTGGGAGTGCGCTATCTGCAGGTGTCTCAGCAGCTCCAGCAGACGAACAGGGTTCTGGAAG

TCACTAACAGCAGCCTGAGGCAGCAGCTCCGCCTCAAGATAACGCAGCTGGGACAGAGTGCAGAGGATCTGCAGGGGTCC

AGGAGAGAGCTGGCGCAGAGTCAGGAAGCACTACAGGTGGAACAGAGGGCTCATCAGGCGGCCGAAGGGCAGCTACAGGC

CTGCCAGGCAGACAGACAGAAGACGAAGGAGACCTTGCAAAGTGAGGAGCAACAGAGGAGGGCCTTGGAGCAGAAGCTGA

GCAACATGGAGAACAGACTGAAGCCCTTCTTCACATGCGGCTCAGCAGACACCTGCTGTCCGTCGGGATGGATAATGCAT

CAGAAAAGCTGCTTTTACATCTCACTTACTTCAAAAAATTGGCAGGAGAGCCAAAAACAATGTGAAACTCTGTCTTCCAA

GCTGGCCACATTCAGTGAAATTTATCCACAATCACACTCTTACTACTTCTTAAATTCACTGTTGCCAAATGGTGGTTCAG

GGAATTCATATTGGACTGGCCTCAGCTCTAACAAGGATTGGAAGTTGACTGATGATACACAACGCACTAGGACTTATGCT

CAAAGCTCAAAATGTAACAAGGTACATAAAACTTGGTCATGGTGGACACTGGAGTCAGAGTCATGTAGAAGTTCTCTTCC

CTACATCTGTGAGATGACAGCTTTCAGGTTTCCAGATTAG

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Proc. Natl. Acad. Sci. 86:1352 (1989)

2. J. Immunol. 144:4870 (1990)

3. Nature 351:662 (1991).

4. J. Immunol. 148:1630 (1992)

5. Immunity 13:621 (2000).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2306-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.