Human OSM Full-length Gene in Lentivector, Endotoxin-free DNA

Product ID: pLTC-OSM (SKU#: LTP1573)

Price:

$696.00

Description

Oncostatin M (OSM) is a member of a cytokine family that includes leukemia-inhibitory factor (LIF), granulocyte colony-stimulating factor (G-CSF), and interleukin 6 (IL-6). The human OSM gene encodes a 252-aa pre-pro-OSM polypeptide with a 25-aa signal peptide and a C-terminal domain that are proteolytically processed to generate the 196-aa mature & active form. OSM is a pleiotropic cytokine that signals through cell surface type I and typ II receptors, which share the similarity to gp130, a signal transducing component of the IL-6, LIF and ciliary neurotrophic factor (CNTF) receptor complexes. OSM plays roles in liver development, hematopoeisis, inflammation, bone formation and destruction, and CNS development. OSM was originally identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It mediates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Like LIF, IL-6 and G-CSF, OSM has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and induce their differentiation into macrophage-like cells. OSM is found primarily in activated T-lymphocyte and monocyte contributing to inflammation. OSM also has growth stimulatory activities on normal fibroblasts, sarcoma cells, and human erythroleukemia cell line, TF-1. Both OSM and LIF are expressed in the uterus around the time of implantation and considered as important factors for the development of early embryos. As a closely related cytokine to LIF, OSM mimics the activity of LIF in the maintenance of embryonic stem (ES) cell pluripotency and germline competency.

Product Details

Gene Symbol: OSM

NCBI Gene ID: 5008

Uniprot Entry: P13725

Construct Details: Full length human OSM gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter, which can be used for both transient and stable expression in mammalian cells. It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

Vector Type: pLTC (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

Gene Insert Size: 759 (bp)

Gene Insert Sequence:

ATGGGGGTACTGCTCACACAGAGGACGCTGCTCAGTCTGGTCCTTGCACTCCTGTTTCCAAGCATGGCGAGCATGGCGGC

TATAGGCAGCTGCTCGAAAGAGTACCGCGTGCTCCTTGGCCAGCTCCAGAAGCAGACAGATCTCATGCAGGACACCAGCA

GACTCCTGGACCCCTATATACGTATCCAAGGCCTGGATGTTCCTAAACTGAGAGAGCACTGCAGGGAGCGCCCCGGGGCC

TTCCCCAGTGAGGAGACCCTGAGGGGGCTGGGCAGGCGGGGCTTCCTGCAGACCCTCAATGCCACACTGGGCTGCGTCCT

GCACAGACTGGCCGACTTAGAGCAGCGCCTCCCCAAGGCCCAGGATTTGGAGAGGTCTGGGCTGAACATCGAGGACTTGG

AGAAGCTGCAGATGGCGAGGCCGAACATCCTCGGGCTCAGGAACAACATCTACTGCATGGCCCAGCTGCTGGACAACTCA

GACACGGCTGAGCCCACGAAGGCTGGCCGGGGGGCCTCTCAGCCGCCCACCCCCACCCCTGCCTCGGATGCTTTTCAGCG

CAAGCTGGAGGGCTGCAGGTTCCTGCATGGCTACCATCGCTTCATGCACTCAGTGGGGCGGGTCTTCAGCAAGTGGGGGG

AGAGCCCGAACCGGAGCCGGAGACACAGCCCCCACCAGGCCCTGAGGAAGGGGGTGCGCAGGACCAGACCCTCCAGGAAA

GGCAAGAGACTCATGACCAGGGGACAGCTGCCCCGGTAG

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CGGGAAGCAATAGCATGATA-3’

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification. High fidelity PCR systems should always be used.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable. Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose. Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules. Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction. Your use of this product constitutes acceptance of the terms of this limited use agreement. Please refer to our “terms & conditions” for details. If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

Storage

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

References