AGRL2 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRL2 may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked.  AGRL2 participates in the regulation of exocytosis. AGRL2 is a calcium-independent receptor of low affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. 

AGRL2 is a 7 transmembrane (7TM) receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRL2 may be proteolytically processed to form a heterodimer of 2 chains: the large extracellular N-terminal fragment and the membrane-bound C-terminal 7TM fragment predominantly remain associated and non-covalently linked.  AGRL2 participates in the regulation of exocytosis. AGRL2 is a calcium-independent receptor of low affinity for alpha-latrotoxin, an excitatory neurotoxin present in black widow spider venom which triggers massive exocytosis from neurons and neuroendocrine cells. 

 

Product Details

 

Gene Symbol: AGRL2; ADGRL2; CL2; LEC1; CIRL2; LPHH1; LPHN2; KIAA0786

 

NCBI Gene ID: 23266

 

Uniprot Entry: Q95490

 

Construct Details: Full length human AGRL2 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4212 (bp)

 

Gene Insert Sequence:  

ATGGTGTCTTCTGGTTGCAGAATGCGAAGTCTGTGGTTTATCATTGTAATCAGCTTCTTACCAAATACAG

AAGGTTTCAGCAGAGCAGCTTTACCATTTGGGCTGGTGAGGCGAGAATTATCCTGTGAAGGTTATTCTAT

AGATCTGCGATGCCCGGGCAGTGATGTCATCATGATTGAGAGCGCTAACTATGGTCGGACGGATGACAAG

ATTTGTGATGCTGACCCATTTCAGATGGAGAATACAGACTGCTACCTCCCCGATGCCTTCAAAATTATGA

CTCAAAGGTGCAACAATCGAACACAGTGTATAGTAGTTACTGGGTCAGATGTGTTTCCTGATCCATGTCC

TGGAACATACAAATACCTTGAAGTCCAATATGAATGTGTCCCTTACATTTTTGTGTGTCCTGGGACCTTG

AAAGCAATTGTGGACTCACCATGTATATATGAAGCTGAACAAAAGGCGGGTGCTTGGTGCAAGGACCCTC

TTCAGGCTGCAGATAAAATTTATTTCATGCCCTGGACTCCCTATCGTACCGATACTTTAATAGAATATGC

TTCTTTAGAAGATTTCCAAAATAGTCGCCAAACAACAACATATAAACTTCCAAATCGAGTAGATGGTACT

GGATTTGTGGTGTATGATGGTGCTGTCTTCTTTAACAAAGAAAGAACGAGGAATATTGTGAAATTTGACT

TGAGGACTAGAATTAAGAGTGGCGAGGCCATAATTAACTATGCCAACTACCATGATACCTCACCATACAG

ATGGGGAGGAAAGACTGATATCGACCTAGCAGTTGATGAAAATGGTTTATGGGTCATTTACGCCACTGAA

CAGAACAATGGAATGATAGTTATTAGCCAGCTGAATCCATACACTCTTCGATTTGAAGCAACGTGGGAGA

CTGTATACGACAAACGTGCCGCATCAAATGCTTTTATGATATGCGGAGTCCTCTATGTGGTTAGGTCAGT

TTATCAAGACAATGAAAGTGAAACAGGCAAGAACTCAATTGATTACATTTATAATACCCGATTAAACCGA

GGAGAATATGTAGATGTTCCCTTCCCCAACCAGTATCAGTATATTGCTGCAGTGGATTACAATCCAAGAG

ATAACCAACTTTACGTGTGGAACAATAACTTCATTTTACGATATTCTCTGGAGTTTGGTCCACCTGATCC

TGCCCAAGTGCCTACCACAGCTGTGACAATAACTTCTTCAGCTGAGCTGTTCAAAACCATAATATCAACC

ACAAGCACTACTTCACAGAAAGGCCCCATGAGCACAACTGTAGCTGGATCACAGGAAGGAAGCAAAGGGA

CAAAACCACCTCCAGCAGTTTCTACAACCAAAATTCCACCTATAACAAATATTTTTCCCCTGCCAGAGAG

ATTCTGTGAAGCATTAGACTCCAAGGGGATAAAGTGGCCTCAGACACAAAGGGGAATGATGGTTGAACGA

CCATGCCCTAAGGGAACAAGAGGAACTGCCTCATATCTCTGCATGATTTCCACTGGAACATGGAACCCTA

AGGGCCCCGATCTTAGCAACTGTACCTCACACTGGGTGAATCAGCTGGCTCAGAAGATCAGAAGCGGAGA

AAATGCTGCTAGTCTTGCCAATGAACTGGCTAAACATACCAAAGGGCCAGTGTTTGCTGGGGATGTAAGT

TCTTCAGTGAGATTGATGGAGCAGTTGGTGGACATCCTTGATGCACAGCTGCAGGAACTGAAACCTAGTG

AAAAAGATTCAGCTGGACGGAGTTATAACAAGGCAATTGTTGACACAGTGGACAACCTTCTGAGACCCGA

AGCTTTGGAATCATGGAAACATATGAATTCTTCTGAACAAGCACATACTGCAACAATGTTACTCGATACA

TTGGAAGAAGGAGCTTTTGTCCTAGCTGACAATCTTTTAGAACCAACAAGGGTCTCAATGCCCACAGAAA

ATATTGTCCTGGAAGTTGCCGTACTCAGTACAGAAGGACAGATCCAAGACTTTAAATTTCCTCTGGGCAT

CAAAGGAGCAGGCAGCTCAATCCAACTGTCCGCAAATACCGTCAAACAGAACAGCAGGAATGGGCTTGCA

AAGTTGGTGTTCATCATTTACCGGAGCCTGGGACAGTTCCTTAGTACAGAAAATGCAACCATTAAACTGG

GTGCTGATTTTATTGGTCGTAATAGCACCATTGCAGTGAACTCTCACGTCATTTCAGTTTCAATCAATAA

AGAGTCCAGCCGAGTATACCTGACTGATCCTGTGCTTTTTACCCTGCCACACATTGATCCTGACAATTAT

TTCAATGCAAACTGCTCCTTCTGGAACTACTCAGAGAGAACTATGATGGGATATTGGTCTACCCAGGGCT

GCAAGCTGGTTGACACTAATAAAACTCGAACAACGTGTGCATGCAGCCACCTAACCAATTTTGCAATTCT

CATGGCCCACAGGGAAATTGCATATAAAGATGGCGTTCATGAATTACTTCTTACAGTCATCACCTGGGTG

GGAATTGTCATTTCCCTTGTTTGCCTGGCTATCTGCATCTTCACCTTCTGCTTTTTCCGTGGCCTACAGA

GTGACCGAAATACTATTCACAAGAACCTTTGTATCAACCTTTTCATTGCTGAATTTATTTTCCTAATAGG

CATTGATAAGACAAAATATGCGATTGCATGCCCAATATTTGCAGGACTTCTACACTTTTTCTTTTTGGCA

GCTTTTGCTTGGATGTGCCTAGAAGGTGTGCAGCTCTACCTAATGTTAGTTGAAGTTTTTGAAAGTGAAT

ATTCAAGGAAAAAATATTACTATGTTGCTGGTTACTTGTTTCCTGCCACAGTGGTTGGAGTTTCAGCTGC

TATTGACTATAAGAGCTATGGAACAGAAAAAGCTTGCTGGCTTCATGTTGATAACTACTTTATATGGAGC

TTCATTGGACCTGTTACCTTCATTATTCTGCTAAATATTATCTTCTTGGTGATCACATTGTGCAAAATGG

TGAAGCATTCAAACACTTTGAAACCAGATTCTAGCAGGTTGGAAAACATTAAGTCTTGGGTGCTTGGCGC

TTTCGCTCTTCTGTGTCTTCTTGGCCTCACCTGGTCCTTTGGGTTGCTTTTTATTAATGAGGAGACTATT

GTGATGGCATATCTCTTCACTATATTTAATGCTTTCCAGGGAGTGTTCATTTTCATCTTTCACTGTGCTC

TCCAAAAGAAAGTACGAAAAGAATATGGCAAGTGCTTCAGACACTCATACTGCTGTGGAGGCCTCCCAAC

TGAGAGTCCCCACAGTTCAGTGAAGGCATCAACCACCAGAACCAGTGCTCGCTATTCCTCTGGCACACAG

AGTCGTATAAGAAGAATGTGGAATGATACTGTGAGAAAACAATCAGAATCTTCTTTTATCTCAGGTGACA

TCAATAGCACTTCAACACTTAATCAAGGACATTCACTGAACAATGCCAGGGATACAAGTGCCATGGATAC

TCTACCGCTAAATGGTAATTTTAACAACAGCTACTCGCTGCACAAGGGTGACTATAATGACAGCGTGCAA

GTTGTGGACTGTGGACTAAGTCTGAATGATACTGCTTTTGAGAAAATGATCATTTCAGAATTAGTGCACA

ACAACTTACGGGGCAGCAGCAAGACTCACAACCTCGAGCTCACGCTACCAGTCAAACCTGTGATTGGAGG

TAGCAGCAGTGAAGATGATGCTATTGTGGCAGATGCTTCATCTTTAATGCACAGCGACAACCCAGGGCTG

GAGCTCCATCACAAAGAACTCGAGGCACCACTTATTCCTCAGCGGACTCACTCCCTTCTGTACCAACCCC

AGAAGAAAGTGAAGTCCGAGGGAACTGACAGCTATGTCTCCCAACTGACAGCAGAGGCTGAAGATCACCT

ACAGTCCCCCAACAGAGACTCTCTTTATACAAGCATGCCCAATCTTAGAGACTCTCCCTATCCGGAGAGC

AGCCCTGACATGGAAGAAGACCTCTCTCCCTCCAGGAGGAGTGAGAATGAGGACATTTACTATAAAAGCA

TGCCAAATCTTGGAGCTGGCCATCAGCTTCAGATGTGCTACCAGATCAGCAGGGGCAATAGTGATGGTTA

TATAATCCCCATTAACAAAGAAGGGTGTATTCCAGAAGGAGATGTTAGAGAAGGACAAATGCAGCTGGTT

ACAAGTCTTTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.