AGRF5 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRF5 is an orphan GPCR involved in cell adhesion and probably in cell-cell interactions.  The adhesion G-protein-coupled receptors (GPCRs), including AGRF5 are membrane-bound proteins with long N termini containing multiple domains. AGRF5 contains 3 Ig-like (immunoglobulin-like) domains. at the N-terminus. AGRF5 may play a critical role in lung surfactant homeostasis and in controlling adipocyte function.

AGRF5 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRF5 is an orphan GPCR involved in cell adhesion and probably in cell-cell interactions.  The adhesion G-protein-coupled receptors (GPCRs), including AGRF5 are membrane-bound proteins with long N termini containing multiple domains. AGRF5 contains 3 Ig-like (immunoglobulin-like) domains. at the N-terminus. AGRF5 may play a critical role in lung surfactant homeostasis and in controlling adipocyte function.

 

Product Details

 

Gene Symbol: AGRF5; ADGRF5; GPR116; KPG_001; KIAA0758

 

NCBI Gene ID: 221395

 

Uniprot Entry: Q8IZF2

 

Construct Details: Full length human AGRF5 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 4041 (bp)

 

Gene Insert Sequence:  

ATGAAATCCCCAAGGAGAACCACTTTGTGCCTCATGTTTATTGTGATTTATTCTTCCAAAGCTGCACTGA

ACTGGAATTACGAGTCTACTATTCATCCTTTGAGTCTTCATGAACATGAACCAGCTGGTGAAGAGGCACT

GAGGCAAAAACGAGCCGTTGCCACAAAAAGTCCTACGGCTGAAGAATACACTGTTAATATTGAGATCAGT

TTTGAAAATGCATCCTTCCTGGATCCTATCAAAGCCTACTTGAACAGCCTCAGTTTTCCAATTCATGGGA

ATAACACTGACCAAATTACCGACATTTTGAGCATAAATGTGACAACAGTCTGCAGACCTGCTGGAAATGA

AATCTGGTGCTCCTGCGAGACAGGTTATGGGTGGCCTCGGGAAAGGTGTCTTCACAATCTCATTTGTCAA

GAGCGTGACGTCTTCCTCCCAGGGCACCATTGCAGTTGCCTTAAAGAACTGCCTCCCAATGGACCTTTTT

GCCTGCTTCAGGAAGATGTTACCCTGAACATGAGAGTCAGACTAAATGTAGGCTTTCAAGAAGACCTCAT

GAACACTTCCTCCGCCCTCTATAGGTCCTACAAGACCGACTTGGAAACAGCGTTCCGGAAGGGTTACGGA

ATTTTACCAGGCTTCAAGGGCGTGACTGTGACAGGGTTCAAGTCTGGAAGTGTGGTTGTGACATATGAAG

TCAAGACTACACCACCATCACTTGAGTTAATACATAAAGCCAATGAACAAGTTGTACAGAGCCTCAATCA

GACCTACAAAATGGACTACAACTCCTTTCAAGCAGTTACTATCAATGAAAGCAATTTCTTTGTCACACCA

GAAATCATCTTTGAAGGGGACACAGTCAGTCTGGTGTGTGAAAAGGAAGTTTTGTCCTCCAATGTGTCTT

GGCGCTATGAAGAACAGCAGTTGGAAATCCAGAACAGCAGCAGATTCTCGATTTACACCGCACTTTTCAA

CAACATGACTTCGGTGTCCAAGCTCACCATCCACAACATCACTCCAGGTGATGCAGGTGAATATGTTTGC

AAACTGATATTAGACATTTTTGAATATGAGTGCAAGAAGAAAATAGATGTTATGCCCATCCAAATTTTGG

CAAATGAAGAAATGAAGGTGATGTGCGACAACAATCCTGTATCTTTGAACTGCTGCAGTCAGGGTAATGT

TAATTGGAGCAAAGTAGAATGGAAGCAGGAAGGAAAAATAAATATTCCAGGAACCCCTGAGACAGACATA

GATTCTAGCTGCAGCAGATACACCCTCAAGGCTGATGGAACCCAGTGCCCAAGCGGGTCGTCTGGAACAA

CAGTCATCTACACTTGTGAGTTCATCAGTGCCTATGGAGCCAGAGGCAGTGCAAACATAAAAGTGACATT

CATCTCTGTGGCCAATCTAACAATAACCCCGGACCCAATTTCTGTTTCTGAGGGACAAAACTTTTCTATA

AAATGCATCAGTGATGTGAGTAACTATGATGAGGTTTATTGGAACACTTCTGCTGGAATTAAAATATACC

AAAGATTTTATACCACGAGGAGGTATCTTGATGGAGCAGAATCAGTACTGACAGTCAAGACCTCGACCAG

GGAGTGGAATGGAACCTATCACTGCATATTTAGATATAAGAATTCATACAGTATTGCAACCAAAGACGTC

ATTGTTCACCCGCTGCCTCTAAAGCTGAACATCATGGTTGATCCTTTGGAAGCTACTGTTTCATGCAGTG

GTTCCCATCACATCAAGTGCTGCATAGAGGAGGATGGAGACTACAAAGTTACTTTCCATACGGGTTCCTC

ATCCCTTCCTGCTGCAAAAGAAGTTAACAAAAAACAAGTGTGCTACAAACACAATTTCAATGCAAGCTCA

GTTTCCTGGTGTTCAAAAACTGTTGATGTGTGTTGTCACTTTACCAATGCTGCTAATAATTCAGTCTGGA

GCCCATCTATGAAGCTGAATCTGGTTCCTGGGGAAAACATCACATGCCAGGATCCCGTAATAGGTGTCGG

AGAGCCGGGGAAAGTCATCCAGAAGCTATGCCGGTTCTCAAACGTTCCCAGCAGCCCTGAGAGTCCCATT

GGCGGGACCATCACTTACAAATGTGTAGGCTCCCAGTGGGAGGAGAAGAGAAATGACTGCATCTCTGCCC

CAATAAACAGTCTGCTCCAGATGGCTAAGGCTTTGATCAAGAGCCCCTCTCAGGATGAGATGCTCCCTAC

ATACCTGAAGGATCTTTCTATTAGCATAGACAAAGCGGAACATGAAATCAGCTCTTCTCCTGGGAGTCTG

GGAGCCATTATTAACATCCTTGATCTGCTCTCAACAGTTCCAACCCAAGTAAATTCAGAAATGATGACGC

ACGTGCTCTCTACGGTTAATGTCATCCTTGGCAAGCCCGTCTTGAACACCTGGAAGGTTTTACAACAGCA

ATGGACCAATCAGAGTTCACAGCTACTACATTCAGTGGAAAGATTTTCCCAAGCATTACAGTCGGGAGAT

AGCCCTCCTTTGTCCTTCTCCCAAACTAATGTGCAGATGAGCAGCATGGTAATCAAGTCCAGCCACCCAG

AAACCTATCAACAGAGGTTTGTTTTCCCATACTTTGACCTCTGGGGCAATGTGGTCATTGACAAGAGCTA

TCTAGAAAACTTGCAGTCGGATTCGTCTATTGTCACCATGGCTTTCCCAACTCTCCAAGCCATCCTTGCC

CAGGATATCCAGGAAAATAACTTTGCAGAGAGCTTAGTGATGACAACCACTGTCAGCCACAATACAACTA

TGCCATTCAGGATTTCAATGACTTTTAAGAACAATAGCCCTTCAGGCGGCGAAACGAAGTGTGTCTTCTG

GAACTTCAGGCTTGCCAACAACACAGGGGGGTGGGACAGCAGTGGGTGCTATGTAGAAGAAGGTGATGGG

GACAATGTCACCTGTATCTGTGACCACCTAACATCATTCTCCATCCTCATGTCCCCTGACTCCCCAGATC

CTAGTTCTCTCCTGGGAATACTCCTGGATATTATTTCTTATGTTGGGGTGGGCTTTTCCATCTTGAGCTT

GGCAGCCTGTCTAGTTGTGGAAGCTGTGGTGTGGAAATCGGTGACCAAGAACCGGACTTCTTATATGCGC

CACACCTGCATAGTGAATATCGCTGCCTCCCTTCTGGTCGCCAACACCTGGTTCATTGTGGTCGCTGCCA

TCCAGGACAATCGCTACATACTCTGCAAGACAGCCTGTGTGGCTGCCACCTTCTTCATCCACTTCTTCTA

CCTCAGCGTCTTCTTCTGGATGCTGACACTGGGCCTCATGCTGTTCTATCGCCTGGTTTTCATTCTGCAT

GAAACAAGCAGGTCCACTCAGAAAGCCATTGCCTTCTGTCTTGGCTATGGCTGCCCACTTGCCATCTCGG

TCATCACGCTGGGAGCCACCCAGCCCCGGGAAGTCTATACGAGGAAGAATGTCTGTTGGCTCAACTGGGA

GGACACCAAGGCCCTGCTGGCTTTCGCCATCCCAGCACTGATCATTGTGGTGGTGAACATAACCATCACT

ATTGTGGTCATCACCAAGATCCTGAGGCCTTCCATTGGAGACAAGCCATGCAAGCAGGAGAAGAGCAGCC

TGTTTCAGATCAGCAAGAGCATTGGGGTCCTCACACCACTCTTGGGCCTCACTTGGGGTTTTGGTCTCAC

CACTGTGTTCCCAGGGACCAACCTTGTGTTCCATATCATATTTGCCATCCTCAATGTCTTCCAGGGATTA

TTCATTTTACTCTTTGGATGCCTCTGGGATCTGAAGGTACAGGAAGCTTTGCTGAATAAGTTTTCATTGT

CGAGATGGTCTTCACAGCACTCAAAGTCAACATCCCTGGGTTCATCCACACCTGTGTTTTCTATGAGTTC

TCCAATATCAAGGAGATTTAACAATTTGTTTGGTAAAACAGGAACGTATAATGTTTCCACCCCAGAAGCA

ACCAGCTCATCCCTGGAAAACTCATCCAGTGCTTCTTCGTTGCTCAACTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.