AGRE1 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRE1 is an orphan GPCR with 6 EGF-like domains involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. AGRE1 may play a role in regulatory T-cells (Treg) development and function.  AGRE1 contains 7 transmembrane (7TM) domains at its C-terminus. The N-terminus of the encoded protein has 6 EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. 

AGRE1 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRE1 is an orphan GPCR with 6 EGF-like domains involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. AGRE1 may play a role in regulatory T-cells (Treg) development and function.  AGRE1 contains 7 transmembrane (7TM) domains at its C-terminus. The N-terminus of the encoded protein has 6 EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. 

 

Product Details

 

Gene Symbol: AGRE1; ADGRE1; EMR1; TM7LN3

 

NCBI Gene ID: 2015

 

Uniprot Entry: Q14246

 

Construct Details: Full length human AGRE1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2661 (bp)

 

Gene Insert Sequence:  

ATGCGTGGCTTCAACCTGCTCCTCTTCTGGGGATGTTGTGTTATGCACAGCTGGGAAGGGCACATAAGAC

CCACACGGAAACCAAACACAAAGGGTAATAACTGTAGAGACAGTACCTTGTGCCCAGCTTATGCCACCTG

CACCAATACAGTGGACAGTTACTATTGCGCTTGCAAACAAGGCTTCCTGTCCAGCAATGGGCAAAATCAC

TTCAAGGATCCAGGAGTGCGATGCAAAGATATTGATGAATGTTCTCAAAGCCCCCAGCCCTGTGGTCCTA

ACTCATCCTGCAAAAACCTGTCAGGGAGGTACAAGTGCAGCTGTTTAGATGGTTTCTCTTCTCCCACTGG

AAATGACTGGGTCCCAGGAAAGCCGGGCAATTTCTCCTGTACTGATATCAATGAGTGCCTCACCAGCAGC

GTCTGCCCTGAGCATTCTGACTGTGTCAACTCCATGGGAAGCTACAGTTGCAGCTGTCAAGTTGGATTCA

TCTCTAGAAACTCCACCTGTGAAGACGTGGATGAATGTGCAGATCCAAGAGCTTGCCCAGAGCATGCAAC

TTGTAATAACACTGTTGGAAACTACTCTTGTTTCTGCAACCCAGGATTTGAATCCAGCAGTGGCCACTTG

AGTTTCCAGGGTCTCAAAGCATCGTGTGAAGATATTGATGAATGCACTGAAATGTGCCCCATCAATTCAA

CATGCACCAACACTCCTGGGAGCTACTTTTGCACCTGCCACCCTGGCTTTGCACCAAGCAATGGACAGTT

GAATTTCACAGACCAAGGAGTGGAATGTAGAGATATTGATGAGTGCCGCCAAGATCCATCAACCTGTGGT

CCTAATTCTATCTGCACCAATGCCCTGGGCTCCTACAGCTGTGGCTGCATTGCAGGCTTTCATCCCAATC

CAGAAGGCTCCCAGAAAGATGGCAACTTCAGCTGCCAAAGGGTTCTCTTCAAATGTAAGGAAGATGTGAT

ACCCGATAATAAGCAGATCCAGCAATGCCAAGAGGGAACCGCAGTGAAACCTGCATATGTCTCCTTTTGT

GCACAAATAAATAACATCTTCAGCGTTCTGGACAAAGTGTGTGAAAATAAAACGACCGTAGTTTCTCTGA

AGAATACAACTGAGAGCTTTGTCCCTGTGCTTAAACAAATATCCACGTGGACTAAATTCACCAAGGAAGA

GACGTCCTCCCTGGCCACAGTCTTCCTGGAGAGTGTGGAAAGCATGACACTGGCATCTTTTTGGAAACCC

TCAGCAAATATCACTCCGGCTGTTCGGACGGAATACTTAGACATTGAGAGCAAAGTTATCAACAAAGAAT

GCAGTGAAGAGAATGTGACGTTGGACTTGGTAGCCAAGGGGGATAAGATGAAGATCGGGTGTTCCACAAT

TGAGGAATCTGAATCCACAGAGACCACTGGTGTGGCTTTTGTCTCCTTTGTGGGCATGGAATCGGTTTTA

AATGAGCGCTTCTTCAAAGACCACCAGGCTCCCTTGACCACCTCTGAGATCAAGCTGAAGATGAATTCTC

GAGTCGTTGGGGGCATAATGACTGGAGAGAAGAAAGACGGCTTCTCAGATCCAATCATCTACACTCTGGA

GAACATTCAGCCAAAGCAGAAGTTTGAGAGGCCCATCTGTGTTTCCTGGAGCACTGATGTGAAGGGTGGA

AGATGGACATCCTTTGGCTGTGTGATCCTGGAAGCTTCTGAGACATATACCATCTGCAGCTGTAATCAGA

TGGCAAATCTTGCCGTTATCATGGCGTCTGGGGAGCTCACGATGGACTTTTCCTTGTACATCATTAGCCA

TGTAGGCATTATCATCTCCTTGGTGTGCCTCGTCTTGGCCATCGCCACCTTTCTGCTGTGTCGCTCCATC

CGAAATCACAACACCTACCTCCACCTGCACCTCTGCGTGTGTCTCCTCTTGGCGAAGACTCTCTTCCTCG

CCGGTATACACAAGACTGACAACAAGATGGGCTGCGCCATCATCGCGGGCTTCCTGCACTACCTTTTCCT

TGCCTGCTTCTTCTGGATGCTGGTGGAGGCTGTGATACTGTTCTTGATGGTCAGAAACCTGAAGGTGGTG

AATTACTTCAGCTCTCGCAACATCAAGATGCTGCACATCTGTGCCTTTGGTTATGGGCTGCCGATGCTGG

TGGTGGTGATCTCTGCCAGTGTGCAGCCACAGGGCTATGGAATGCATAATCGCTGCTGGCTGAATACAGA

GACAGGGTTCATCTGGAGTTTCTTGGGGCCAGTTTGCACAGTTATAGTGATCAACTCCCTTCTCCTGACC

TGGACCTTGTGGATCCTGAGGCAGAGGCTTTCCAGTGTTAATGCCGAAGTCTCAACGCTAAAAGACACCA

GGTTACTGACCTTCAAGGCCTTTGCCCAGCTCTTCATCCTGGGCTGCTCCTGGGTGCTGGGCATTTTTCA

GATTGGACCTGTGGCAGGTGTCATGGCTTACCTGTTCACCATCATCAACAGCCTGCAGGGGGCCTTCATC

TTCCTCATCCACTGTCTGCTCAACGGCCAGGTACGAGAAGAATACAAGAGGTGGATCACTGGGAAGACGA

AGCCCAGCTCCCAGTCCCAGACCTCAAGGATCTTGCTGTCCTCCATGCCATCCGCTTCCAAGACGGGTTA

A

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.