AGRA3 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRA3 may play a role in tumor angiogenesis through its interaction with the human homolog of the Drosophila disc large tumor suppressor gene. This gene is mapped to a candidate region of chromosome 4 which may be associated with bipolar disorder and schizophrenia.

AGRA3 is a 7 transmembrane receptor belonging to the secretin receptor family, which is also known as family B or 2 of the G-protein-coupled receptors (GPCRs). Many members of this family are receptors for peptide hormones from the glucagon hormone family and regulate the activation of two major signal transduction pathways: adenylyl cyclase (cAMP) and phosphatidylinositol-calcium pathways. AGRA3 may play a role in tumor angiogenesis through its interaction with the human homolog of the Drosophila disc large tumor suppressor gene. This gene is mapped to a candidate region of chromosome 4 which may be associated with bipolar disorder and schizophrenia.

 

Product Details

 

Gene Symbol: AGRA3; ADGRA3; PGR21; TEM5L; GPR125; UNQ556/PRO1113

 

NCBI Gene ID: 166647

 

Uniprot Entry: Q8IWK6

 

Construct Details: Full length human AGRA3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 3966 (bp)

 

Gene Insert Sequence:  

ATGGAGCCACCCGGACGCCGGCGGGGCCGCGCGCAGCCGCCGCTGTTGCTGCCGCTCTCGCTGTTAGCGC

TGCTCGCGCTGCTGGGAGGCGGCGGCGGCGGCGGCGCCGCGGCGCTGCCCGCCGGCTGCAAGCACGATGG

GCGGCCCCGAGGGGCTGGCAGGGCGGCGGGCGCCGCCGAGGGCAAGGTGGTGTGCAGCAGCCTGGAACTC

GCGCAGGTCCTGCCCCCAGATACTCTGCCCAACCGCACGGTCACCCTGATTCTGAGTAACAATAAGATAT

CCGAGCTGAAGAATGGCTCATTTTCTGGGTTAAGTCTCCTTGAAAGATTGGACCTCCGAAACAATCTTAT

TAGTAGTATAGATCCAGGTGCCTTCTGGGGACTGTCATCTCTAAAAAGATTGGATCTGACAAACAATCGA

ATAGGATGTCTGAATGCAGACATATTTCGAGGACTCACCAATCTGGTTCGGCTAAACCTTTCGGGGAATT

TGTTTTCTTCATTATCTCAAGGAACTTTTGATTATCTTGCGTCATTACGGTCTTTGGAATTCCAGACTGA

GTATCTTTTGTGTGACTGTAACATACTGTGGATGCATCGCTGGGTAAAGGAGAAGAACATCACGGTACGG

GATACCAGGTGTGTTTATCCTAAGTCACTGCAGGCCCAACCAGTCACAGGCGTGAAGCAGGAGCTGTTGA

CATGCGACCCTCCGCTTGAATTGCCGTCTTTCTACATGACTCCATCTCATCGCCAAGTTGTGTTTGAAGG

AGACAGCCTTCCTTTCCAGTGCATGGCTTCATATATTGATCAGGACATGCAAGTGTTGTGGTATCAGGAT

GGGAGAATAGTTGAAACCGATGAATCGCAAGGTATTTTTGTTGAAAAGAACATGATTCACAACTGCTCCT

TGATTGCAAGTGCCCTAACCATTTCTAATATTCAGGCTGGATCTACTGGAAATTGGGGCTGTCATGTCCA

GACCAAACGTGGGAATAATACGAGGACTGTGGATATTGTGGTATTAGAGAGTTCTGCACAGTACTGTCCT

CCAGAGAGGGTGGTAAACAACAAAGGTGACTTCAGATGGCCCAGAACATTGGCAGGCATTACTGCATATC

TGCAGTGTACGCGGAACACCCATGGCAGTGGGATATATCCCGGAAACCCACAGGATGAGAGAAAAGCTTG

GCGCAGATGTGATAGAGGTGGCTTTTGGGCAGATGATGATTATTCTCGCTGTCAGTATGCAAATGATGTC

ACTAGAGTTCTTTATATGTTTAATCAGATGCCCCTCAATCTTACCAATGCCGTGGCAACAGCTCGACAGT

TACTGGCTTACACTGTGGAAGCAGCCAACTTTTCTGACAAAATGGATGTTATATTTGTGGCAGAAATGAT

TGAAAAATTTGGAAGATTTACCAAGGAGGAAAAATCAAAAGAGCTAGGTGACGTGATGGTTGACATTGCA

AGTAACATCATGTTGGCTGATGAACGTGTCCTGTGGCTGGCGCAGAGGGAAGCTAAAGCCTGCAGTAGGA

TTGTGCAGTGTCTTCAGCGCATTGCTACCTACCGGCTAGCCGGTGGAGCTCACGTTTATTCAACATATTC

ACCCAATATTGCTCTGGAAGCTTATGTCATCAAGTCTACTGGCTTCACGGGGATGACCTGTACCGTGTTC

CAGAAAGTGGCAGCCTCTGATCGTACAGGACTTTCGGATTATGGGAGGCGGGATCCAGAGGGAAACCTGG

ATAAGCAGCTGAGCTTTAAGTGCAATGTTTCAAATACATTTTCGAGTCTGGCACTAAAGAATACTATTGT

GGAGGCTTCTATTCAGCTTCCTCCTTCCCTTTTCTCACCAAAGCAAAAAAGAGAACTCAGACCAACTGAT

GACTCTCTTTACAAGCTTCAACTCATTGCATTCCGCAATGGAAAGCTTTTTCCAGCCACTGGAAATTCAA

CAAATTTGGCTGATGATGGAAAACGACGTACTGTGGTTACCCCTGTGATTCTCACCAAAATAGATGGTGT

GAATGTAGATACCCACCACATCCCTGTTAATGTGACACTGCGTCGAATTGCACATGGAGCAGATGCTGTT

GCAGCCCGGTGGGATTTCGATTTGCTGAACGGACAAGGAGGCTGGAAGTCAGATGGGTGCCATATACTCT

ATTCAGATGAAAATATCACTACGATTCAGTGCTACTCCCTTAGTAACTATGCAGTTTTAATGGATTTGAC

GGGATCTGAACTATACACCCAGGCGGCCAGCCTCCTGCATCCTGTGGTTTATACTACCGCTATCATTCTC

CTCTTATGTCTCTTAGCCGTCATTGTCAGTTACATATACCATCACAGTTTGATTAGAATCAGCCTCAAGA

GCTGGCACATGCTTGTGAACTTGTGCTTTCATATTTTCCTAACCTGTGTGGTCTTTGTGGGAGGAATAAC

CCAGACTAGGAATGCCAGCATCTGCCAAGCAGTTGGGATAATTCTTCACTATTCCACCCTTGCCACAGTA

CTATGGGTAGGAGTGACAGCTCGAAATATCTACAAACAAGTCACTAAAAAAGCTAAAAGATGCCAGGATC

CTGATGAACCACCACCTCCACCAAGACCAATGCTCAGATTTTACCTGATTGGTGGTGGTATCCCCATCAT

TGTTTGCGGCATAACTGCAGCAGCGAACATTAAGAATTACGGCAGTCGGCCAAACGCACCCTATTGCTGG

ATGGCATGGGAACCCTCCTTGGGAGCCTTCTATGGGCCAGCCAGCTTCATCACTTTTGTAAACTGCATGT

ACTTTCTGAGCATATTTATTCAGTTGAAAAGACACCCTGAGCGCAAATATGAGCTTAAGGAGCCCACGGA

GGAGCAACAGAGATTGGCAGCCAATGAAAATGGCGAAATAAATCATCAGGATTCAATGTCTTTGTCTCTG

ATTTCTACATCAGCCTTGGAAAATGAGCACACTTTTCATTCTCAGCTCTTGGGGGCCAGCCTTACTTTGC

TCTTATATGTTGCACTGTGGATGTTTGGGGCTTTGGCTGTTTCTTTGTATTACCCTTTGGACTTGGTTTT

TAGCTTCGTTTTTGGAGCCACAAGTTTAAGCTTCAGTGCGTTCTTCGTGGTCCACCATTGTGTTAATAGG

GAGGATGTTAGACTTGCGTGGATCATGACTTGCTGCCCAGGACGGAGCTCGTATTCAGTGCAAGTCAACG

TCCAGCCCCCCAACTCTAATGGGACGAATGGAGAGGCACCCAAATGCCCCAATAGCAGTGCGGAGTCTTC

ATGCACAAACAAAAGTGCTTCAAGCTTCAAAAATTCCTCCCAGGGCTGCAAATTAACAAACTTGCAGGCG

GCTGCAGCTCAGTGCCATGCCAATTCTTTACCTTTGAACTCCACCCCTCAGCTTGATAATAGTCTGACAG

AACATTCAATGGACAATGATATTAAAATGCACGTGGCGCCTTTAGAAGTTCAGTTTCGAACAAATGTGCA

CTCAAGCCGCCACCATAAAAACAGAAGTAAAGGACACCGGGCAAGCCGACTCACAGTCCTGAGAGAATAT

GCCTACGATGTCCCAACGAGCGTGGAAGGAAGCGTGCAGAACGGCTTACCTAAAAGCCGGCTGGGCAATA

ACGAAGGACACTCGAGGAGCCGAAGAGCTTATTTAGCCTACAGAGAGAGACAGTACAACCCACCCCAGCA

AGACAGCAGCGATGCTTGTAGCACACTTCCCAAAAGTAGCAGAAATTTTGAAAAGCCAGTTTCAACCACT

AGTAAAAAAGATGCGTTAAGGAAGCCAGCTGTGGTTGAACTTGAAAATCAGCAAAAATCTTATGGCCTCA

ACTTGGCCATTCAGAATGGACCAATTAAAAGCAATGGGCAGGAGGGACCCTTGCTCGGTACCGATAGCAC

TGGCAATGTTAGGACTGGATTATGGAAACACGAAACTACTGTGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

1. Ann Rev Biochem 56: 615 (1987)

2. Genome Biol. 2: S3013 (2001)

3. Science 301: 361 (2003)

4. Physiol Rev 85: 1159 (2005)

5. Genomics 88: 263 (2006)

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.