LINGO4 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 4) is also known as LRRN6D (Leucine-rich repeat neuronal protein 6D). LINGO4 is a single-pass type I transmembrane protein with an extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain.

LINGO4 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 4) is also known as LRRN6D (Leucine-rich repeat neuronal protein 6D). LINGO4 is a single-pass type I transmembrane protein with an extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain.

 

Product Details

 

Gene Symbol: LINGO4; LRRN6D; DAAT9248; PRO34002

 

NCBI Gene ID: 339398

 

Uniprot Entry: Q6UY18

 

Construct Details: Full length human LINGO4 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1782 (bp)

 

Gene Insert Sequence:  

ATGGATGCAGCCACAGCTCCAAAGCAAGCCTGGCCCCCATGGCCCCCGCTCCTTTTCCTCCTCCTCCTAC

CTGGAGGGAGCGGTGGCAGCTGCCCTGCTGTGTGTGACTGCACCTCCCAGCCCCAGGCTGTGCTCTGTGG

CCACAGGCAACTGGAGGCTGTACCTGGAGGACTCCCACTGGACACTGAGCTCCTGGACCTGAGTGGGAAC

CGCCTGTGGGGGCTCCAGCAGGGAATGCTCTCCCGCCTGAGCCTGCTCCAGGAATTGGACCTCAGCTACA

ACCAGCTCTCAACCCTTGAGCCTGGGGCCTTCCATGGCCTACAAAGCCTACTCACCCTGAGGCTGCAGGG

CAATCGGCTCAGAATCATGGGGCCTGGGGTCTTCTCAGGCCTCTCTGCTCTGACCCTGCTGGACCTCCGC

CTCAACCAGATTGTTCTCTTCCTAGATGGAGCTTTTGGGGAGCTAGGCAGCCTCCAGAAGCTGGAGGTTG

GGGACAACCACCTGGTATTTGTGGCTCCGGGGGCCTTTGCAGGGCTAGCCAAGTTGAGCACCCTCACCCT

GGAGCGCTGCAACCTCAGCACAGTGCCTGGCCTAGCCCTTGCCCGTCTCCCGGCACTAGTGGCCCTAAGG

CTTAGAGAACTGGATATTGGGAGGCTGCCAGCTGGGGCCCTGCGGGGGCTGGGGCAGCTCAAGGAGCTGG

AGATCCACCTCTGGCCATCTCTGGAGGCTCTGGACCCTGGGAGCCTGGTTGGGCTCAATCTCAGCAGCCT

GGCCATCACTCGCTGCAATCTGAGCTCGGTGCCCTTCCAAGCACTGTACCACCTCAGCTTCCTCAGGGTC

CTGGATCTGTCCCAGAATCCCATCTCAGCCATCCCAGCCCGAAGGCTCAGCCCCCTGGTGCGGCTCCAGG

AGCTACGCCTGTCAGGGGCATGCCTCACCTCCATTGCTGCCCATGCCTTCCATGGCTTGACTGCCTTCCA

CCTCCTGGATGTGGCAGATAACGCCCTTCAGACACTAGAGGAAACAGCTTTCCCTTCTCCAGACAAACTG

GTCACCTTGAGGCTGTCTGGCAACCCCCTAACCTGTGACTGCCGCCTCCTCTGGCTGCTCCGGCTCCGCC

GCCACCTGGACTTTGGCATGTCCCCCCCTGCCTGTGCTGGCCCCCATCATGTCCAGGGGAAGAGCCTGAA

GGAGTTTTCAGACATCCTGCCTCCAGGGCACTTCACCTGCAAACCAGCCCTGATCCGAAAGTCGGGGCCT

CGATGGGTCATTGCAGAGGAGGGCGGGCATGCGGTTTTCTCCTGCTCTGGAGATGGAGACCCAGCCCCCA

CTGTCTCCTGGATGAGGCCTCATGGGGCTTGGCTGGGCAGGGCTGGGAGAGTAAGGGTCCTAGAGGATGG

GACACTGGAGATCCGCTCAGTGCAGCTACGGGACAGAGGGGCCTATGTCTGTGTGGTTAGCAATGTCGCT

GGGAATGACTCCCTGAGGACCTGGCTGGAAGTCATCCAGGTGGAACCACCAAACGGCACACTTTCTGACC

CCAACATCACCGTGCCAGGGATCCCAGGGCCTTTTTTTCTGGATAGCAGAGGTGTGGCCATGGTGCTGGC

AGTCGGCTTCCTCCCCTTCCTCACCTCAGTGACCCTCTGCTTTGGCCTGATTGCCCTTTGGAGCAAGGGC

AAAGGTCGGGTCAAACATCACATGACCTTTGACTTTGTGGCACCTCGGCCCTCTGGGGATAAAAACTCTG

GGGGTAACCGGGTCACTGCCAAGCTCTTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. 

 

References

 

1. Eur. J. Neurosci. 18:3167 (2003) 

2. Nat. Neurosci. 7:221-228 (2004) 

3. Nat. Neurosci. 8:745-751 (2005)

4. Neuron 45:345-351 (2005)

5. J. Biol. Chem. 281:36378 (2006) 

6. Proc. Natl. Acad. Sci. 104:14430 (2007)

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.