LINGO3 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 3) is also known as LERN2 (Leucine-rich repeat neuronal protein 2) and LRRN6B (Leucine-rich repeat neuronal protein 6B). LINGO3 is a single-pass type I transmembrane protein with an extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain.

LINGO3 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 3) is also known as LERN2 (Leucine-rich repeat neuronal protein 2) and LRRN6B (Leucine-rich repeat neuronal protein 6B). LINGO3 is a single-pass type I transmembrane protein with an extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain.

 

Product Details

 

Gene Symbol: LINGO3; LERN2; LRRN6B 

 

NCBI Gene ID: 645191

 

Uniprot Entry: P0C658

 

Construct Details: Full length human LINGO3 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1779 (bp)

 

Gene Insert Sequence:  

ATGACCTGCTGGCTGTGCGTCCTGAGCCTGCCCCTGCTCCTGCTGCCCGCGGCGCCGCCCCCGGCTGGAG

GCTGCCCGGCCCGCTGCGAGTGCACCGTGCAGACCCGCGCGGTGGCCTGCACGCGCCGCCGCCTGACCGC

CGTGCCCGACGGCATCCCGGCCGAGACCCGCCTGCTGGAGCTCAGCCGCAACCGCATCCGCTGCCTGAAC

CCGGGCGACCTGGCCGCGCTGCCCGCGCTGGAGGAGCTGGACCTGAGCGAGAACGCCATCGCGCACGTGG

AGCCCGGCGCCTTCGCCAACCTGCCGCGCCTGCGCGTCCTGCGTCTCCGTGGCAACCAGCTGAAGCTCAT

CCCGCCCGGGGTCTTCACGCGCCTGGACAACCTCACGCTGCTGGACCTGAGCGAGAACAAGCTGGTAATC

CTGCTGGACTACACTTTCCAGGACCTGCACAGCCTGCGCCGGCTGGAAGTGGGCGACAACGACCTGGTAT

TCGTCTCGCGCCGCGCCTTCGCGGGGCTGCTGGCCCTGGAGGAGCTGACCCTGGAGCGCTGCAACCTCAC

GGCTCTGTCCGGGGAGTCGCTGGGCCATCTGCGCAGCCTGGGCGCCCTGCGGCTGCGCCACCTGGCCATC

GCCTCCCTGGAGGACCAGAACTTCCGCAGGCTGCCCGGGCTGCTGCACCTGGAGATTGACAACTGGCCGC

TGCTGGAGGAGGTGGCGGCGGGCAGCCTGCGGGGCCTGAACCTGACCTCGCTGTCGGTCACCCACACCAA

CATCACCGCCGTGCCGGCCGCCGCGCTGCGGCACCAGGCGCACCTCACCTGCCTCAATCTGTCGCACAAC

CCCATCAGCACGGTGCCGCGGGGGTCGTTCCGGGACCTGGTCCGCCTGCGCGAGCTGCACCTGGCCGGGG

CCCTGCTGGCTGTGGTGGAGCCGCAGGCCTTCCTGGGCCTGCGCCAGATCCGCCTGCTCAACCTCTCCAA

CAACCTGCTCTCCACGTTGGAGGAGAGCACCTTCCACTCGGTGAACACGCTAGAGACGCTGCGCGTGGAC

GGGAACCCGCTGGCCTGCGACTGTCGCCTGCTGTGGATCGTGCAGCGTCGCAAGACCCTCAACTTCGACG

GGCGGCTGCCGGCCTGCGCCACCCCGGCCGAGGTGCGCGGCGACGCGCTGCGAAACCTGCCGGACTCCGT

GCTGTTCGAGTACTTCGTGTGCCGCAAACCCAAGATCCGGGAGCGGCGGCTGCAGCGCGTCACGGCCACC

GCGGGCGAAGACGTCCGCTTCCTCTGCCGCGCCGAGGGCGAGCCGGCGCCCACCGTGGCCTGGGTGACCC

CCCAGCACCGGCCGGTGACGGCCACCAGCGCGGGCCGGGCGCGCGTGCTCCCCGGGGGGACGCTGGAGAT

CCAGGACGCGCGGCCGCAGGACAGCGGCACCTACACGTGCGTGGCCAGCAACGCGGGCGGCAACGACACC

TACTTCGCCACGCTGACCGTGCGCCCCGAGCCGGCCGCCAACCGGACCCCGGGCGAGGCCCACAACGAGA

CGCTGGCGGCCCTGCGCGCGCCGCTCGACCTCACCACCATCCTGGTGTCCACCGCCATGGGCTGCATCAC

CTTCCTGGGCGTGGTCCTCTTCTGCTTCGTGCTGCTGTTCGTGTGGAGCCGCGGCCGCGGGCAGCACAAA

AACAACTTCTCGGTGGAGTACTCCTTCCGCAAGGTGGATGGGCCGGCCGCCGCGGCGGGCCAGGGAGGCG

CGCGCAAGTTCAACATGAAGATGATCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. 

 

References

 

1. Eur. J. Neurosci. 18:3167 (2003) 

2. Nat. Neurosci. 7:221-228 (2004) 

3. Nat. Neurosci. 8:745-751 (2005)

4. Neuron 45:345-351 (2005)

5. J. Biol. Chem. 281:36378 (2006) 

6. Proc. Natl. Acad. Sci. 104:14430 (2007)

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.