RTN4R (Reticulon-4 receptor) ia a GPI-anchored membrane protein that belongs to the Nogo receptor (NgR) family.  It has a 421 amino acid (aa) extracellular region with 8 LRR (leucine-rich) repeats. RTN4R is widespread in the brain but highest levels in the gray matter. Low levels can be detected in heart and kidney but RTN4R is not expressed in oligodendrocytes (white matter).  RTN4R acts as a receptor for reticulon 4 (RTN4), oligodendrocyte myelin glycoprotein (OMG) and myelin-associated glycoprotein (MAG). RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system (CNS).  RTN4R mediates the activation of Rho and downstream reorganization of the actin cytoskeleton.  RTN4R acts in conjunction with RTN4 and LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) in regulating neuronal precursor cell motility during cortical development.  RTN4R exists as a homomultimer and interacts with LINGO1, KIAA0319L, and NGFR. RTN4R also interacts with OLFM1, which inhibits interaction with LINGO1 and NGFR.

RTN4R (Reticulon-4 receptor) ia a GPI-anchored membrane protein that belongs to the Nogo receptor (NgR) family.  It has a 421 amino acid (aa) extracellular region with 8 LRR (leucine-rich) repeats. RTN4R is widespread in the brain but highest levels in the gray matter. Low levels can be detected in heart and kidney but RTN4R is not expressed in oligodendrocytes (white matter).  RTN4R acts as a receptor for reticulon 4 (RTN4), oligodendrocyte myelin glycoprotein (OMG) and myelin-associated glycoprotein (MAG). RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system (CNS).  RTN4R mediates the activation of Rho and downstream reorganization of the actin cytoskeleton.  RTN4R acts in conjunction with RTN4 and LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) in regulating neuronal precursor cell motility during cortical development.  RTN4R exists as a homomultimer and interacts with LINGO1, KIAA0319L, and NGFR. RTN4R also interacts with OLFM1, which inhibits interaction with LINGO1 and NGFR.

 

Product Details

 

Gene Symbol: RTN4R; NGR; NOGOR; UNQ330/PRO526

 

NCBI Gene ID: 65078

 

Uniprot Entry: Q9BZR6

 

Construct Details: Full length human RTN4R gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1422 (bp)

 

Gene Insert Sequence:  

ATGAAGAGGGCGTCCGCTGGAGGGAGCCGGCTGCTGGCATGGGTGCTGTGGCTGCAGGCCTGGCAGGTGG

CAGCCCCATGCCCAGGTGCCTGCGTATGCTACAATGAGCCCAAGGTGACGACAAGCTGCCCCCAGCAGGG

CCTGCAGGCTGTGCCCGTGGGCATCCCTGCTGCCAGCCAGCGCATCTTCCTGCACGGCAACCGCATCTCG

CATGTGCCAGCTGCCAGCTTCCGTGCCTGCCGCAACCTCACCATCCTGTGGCTGCACTCGAATGTGCTGG

CCCGAATTGATGCGGCTGCCTTCACTGGCCTGGCCCTCCTGGAGCAGCTGGACCTCAGCGATAATGCACA

GCTCCGGTCTGTGGACCCTGCCACATTCCACGGCCTGGGCCGCCTACACACGCTGCACCTGGACCGCTGC

GGCCTGCAGGAGCTGGGCCCGGGGCTGTTCCGCGGCCTGGCTGCCCTGCAGTACCTCTACCTGCAGGACA

ACGCGCTGCAGGCACTGCCTGATGACACCTTCCGCGACCTGGGCAACCTCACACACCTCTTCCTGCACGG

CAACCGCATCTCCAGCGTGCCCGAGCGCGCCTTCCGTGGGCTGCACAGCCTCGACCGTCTCCTACTGCAC

CAGAACCGCGTGGCCCATGTGCACCCGCATGCCTTCCGTGACCTTGGCCGCCTCATGACACTCTATCTGT

TTGCCAACAATCTATCAGCGCTGCCCACTGAGGCCCTGGCCCCCCTGCGTGCCCTGCAGTACCTGAGGCT

CAACGACAACCCCTGGGTGTGTGACTGCCGGGCACGCCCACTCTGGGCCTGGCTGCAGAAGTTCCGCGGC

TCCTCCTCCGAGGTGCCCTGCAGCCTCCCGCAACGCCTGGCTGGCCGTGACCTCAAACGCCTAGCTGCCA

ATGACCTGCAGGGCTGCGCTGTGGCCACCGGCCCTTACCATCCCATCTGGACCGGCAGGGCCACCGATGA

GGAGCCGCTGGGGCTTCCCAAGTGCTGCCAGCCAGATGCCGCTGACAAGGCCTCAGTACTGGAGCCTGGA

AGACCAGCTTCGGCAGGCAATGCGCTGAAGGGACGCGTGCCGCCCGGTGACAGCCCGCCGGGCAACGGCT

CTGGCCCACGGCACATCAATGACTCACCCTTTGGGACTCTGCCTGGCTCTGCTGAGCCCCCGCTCACTGC

AGTGCGGCCCGAGGGCTCCGAGCCACCAGGGTTCCCCACCTCGGGCCCTCGCCGGAGGCCAGGCTGTTCA

CGCAAGAACCGCACCCGCAGCCACTGCCGTCTGGGCCAGGCAGGCAGCGGGGGTGGCGGGACTGGTGACT

CAGAAGGCTCAGGTGCCCTACCCAGCCTCACCTGCAGCCTCACCCCCCTGGGCCTGGCGCTGGTGCTGTG

GACAGTGCTTGGGCCCTGCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. 

 

References

 

1. Nature 409:341 (2001) 

2. Nature 417:547 (2002)

3. Nature 417:941 (2002)

4. Science 297:1190 (2002)

5. J. Neurosci. Res. 67:559 2002) 

6. Nat. Neurosci. 7:221 (2004)

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.