SCF (stem cell factor), also known as KITL/KITLG/KL (c­kit ligand), MGF (mast cell growth factor), and steel factor (SLF), is a single-pass type I transmembrane protein belonging to to the SCF family of dimeric transmembrane growth factors. Mature human SCF is composed of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail.  Proteolytic cleavage releases a 25 kDa soluble protein that shows both N­linked and O­linked glycosylation.  SCF is a primary growth factor that promotes the survival, differentiation, and mobilization of multiple cell types, including mast cells, myeloid, erythroid, megakaryocytic, lymphoid, germ cell, eosinophils and melanocytes. For example, mast cell development depends on SCF and its receptor, c-Kit, which is a receptor tyrosine kinase. Mast cells participate in a variety of immune responses, such as parasite resistance and allergic reactions. SCF also increases the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture.  The noncovalent dimers of transmembrane or soluble form of SCF interact with c­kit to trigger receptor dimerization and signaling activation. SCF may assist in the recovery of cardiac function following myocardial infarction.  It also plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin with the potential for CSF-dependent treatment of pathological skin conditions. The mutations in SCF associate wih hyperpigmentation with or without hypopigmentation, familial progressive (FPHH).

SCF (stem cell factor), also known as KITL/KITLG/KL (c­kit ligand), MGF (mast cell growth factor), and steel factor (SLF), is a single-pass type I transmembrane protein belonging to to the SCF family of dimeric transmembrane growth factors. Mature human SCF is composed of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail.  Proteolytic cleavage releases a 25 kDa soluble protein that shows both N­linked and O­linked glycosylation.  SCF is a primary growth factor that promotes the survival, differentiation, and mobilization of multiple cell types, including mast cells, myeloid, erythroid, megakaryocytic, lymphoid, germ cell, eosinophils and melanocytes. For example, mast cell development depends on SCF and its receptor, c-Kit, which is a receptor tyrosine kinase. Mast cells participate in a variety of immune responses, such as parasite resistance and allergic reactions. SCF also increases the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture.  The noncovalent dimers of transmembrane or soluble form of SCF interact with c­kit to trigger receptor dimerization and signaling activation. SCF may assist in the recovery of cardiac function following myocardial infarction.  It also plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin with the potential for CSF-dependent treatment of pathological skin conditions. The mutations in SCF associate wih hyperpigmentation with or without hypopigmentation, familial progressive (FPHH).

 

Product Details

 

Gene Symbol: SCF; KITLG; SF; SLF; MGF; FPH2; FPHH; KL-1; Kitl; SHEP7

 

NCBI Gene ID: 4254

 

Uniprot Entry: P21583

 

Construct Details: The recombinant human SCF-Fc fusion is expressed as a 396 amino acid protein consisting of Glu26 - Gly184 region of SCF220 isoform (UniProt#P21583-2) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing SCF-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL 

        70         80         90        100        110        120 

DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN 

       130        140        150        160        170        180 

RSIDAFKDFV VASETSDCVV SSTLSPEKGK AKNPPGDSST TENLYFQGST GTHTCPPCPA 

       190        200        210        220        230        240 

PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP 

       250        260        270        280        290        300 

REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL  

       310        320        330        340        350        360 

PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT  

       370        380        390 

VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK 

 

M.W.: Calculated molecular mass (kDa): 44.4; Estimated by SDS-PAGE under reducing condition (kDa): 55-60

 

Calculated PI: 5.98

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 47495

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to human c-Kit in a functional ELISA and stimulates TF-1 human erythroleukemic cell proliferation. 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 63:203 (1990)

2. J. Biol. Chem. 266:18942 (1991)

3. Proc. Natl. Acad. Sci. 88:4671 (1991)

4. Blood. 99: 1866 (2002)

5. Pigment Cell Res.16: 287 (2003)

6. Cardiovasc. Res. 70:117 (2006)

 

 

 

Documentation

 

 

 

Additional supporting documents, including PDS, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.