LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) is also known as LERN1 (Leucine-rich repeat neuronal protein 1) and LRRN6A (Leucine-rich repeat neuronal protein 6A). LINGO1 is a single-pass type I transmembrane protein with a 520 amino acid (aa) extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain. LINGO1 is expressed exclusively in the central nervous system (CNS) with the hghest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression can be detected. LINGO1 is up-regulated in substantia nigra neurons from Parkinson disease patients. LINGO1 is thought to be a functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation.  The receptor complex is responsible for negative regulation of axonal regeneration by myelin-associated factors. LINGO1 may exist as a homotetramer and form a ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. The intracellular domain of LINGO1 interacts with MYT1L for signal transduction. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Lingo1 acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.  Inhibition of LINGO1 enhances survival, structure, and function of dopaminergic neurons in Parkinson's disease models.

LINGO1 (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1) is also known as LERN1 (Leucine-rich repeat neuronal protein 1) and LRRN6A (Leucine-rich repeat neuronal protein 6A). LINGO1 is a single-pass type I transmembrane protein with a 520 amino acid (aa) extracellular region that contains 11 LRR (leucine-rich) repeats and 1 Ig (immunoglobulin)-like C2-type domain. LINGO1 is expressed exclusively in the central nervous system (CNS) with the hghest level in the in amygdala, hippocampus, thalamus and cerebral cortex. In the rest of the brain a basal expression can be detected. LINGO1 is up-regulated in substantia nigra neurons from Parkinson disease patients. LINGO1 is thought to be a functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation.  The receptor complex is responsible for negative regulation of axonal regeneration by myelin-associated factors. LINGO1 may exist as a homotetramer and form a ternary complex with RTN4R/NGFR and RTN4R/TNFRSF19. The intracellular domain of LINGO1 interacts with MYT1L for signal transduction. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. Lingo1 acts in conjunction with RTN4 and RTN4R in regulating neuronal precursor cell motility during cortical development.  Inhibition of LINGO1 enhances survival, structure, and function of dopaminergic neurons in Parkinson's disease models.

 

Product Details

 

Gene Symbol: LINGO1; LERN1; LRRN6A; UNQ201; UNQ201/PRO227

 

NCBI Gene ID: 84894

 

Uniprot Entry: Q96FE5

 

Construct Details: Full length human LINGO1 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 1863 (bp)

 

Gene Insert Sequence:  

ATGCAGGTGAGCAAGAGGATGCTGGCGGGGGGCGTGAGGAGCATGCCCAGCCCCCTCCTGGCCTGCTGGC

AGCCCATCCTCCTGCTGGTGCTGGGCTCAGTGCTGTCAGGCTCGGCCACGGGCTGCCCGCCCCGCTGCGA

GTGCTCCGCCCAGGACCGCGCTGTGCTGTGCCACCGCAAGCGCTTTGTGGCAGTCCCCGAGGGCATCCCC

ACCGAGACGCGCCTGCTGGACCTAGGCAAGAACCGCATCAAAACGCTCAACCAGGACGAGTTCGCCAGCT

TCCCGCACCTGGAGGAGCTGGAGCTCAACGAGAACATCGTGAGCGCCGTGGAGCCCGGCGCCTTCAACAA

CCTCTTCAACCTCCGGACGCTGGGTCTCCGCAGCAACCGCCTGAAGCTCATCCCGCTAGGCGTCTTCACT

GGCCTCAGCAACCTGACCAAGCTGGACATCAGCGAGAACAAGATCGTTATCCTACTGGACTACATGTTTC

AGGACCTGTACAACCTCAAGTCACTGGAGGTTGGCGACAATGACCTCGTCTACATCTCTCACCGCGCCTT

CAGCGGCCTCAACAGCCTGGAGCAGCTGACGCTGGAGAAATGCAACCTGACCTCCATCCCCACCGAGGCG

CTGTCCCACCTGCACGGCCTCATCGTCCTGAGGCTCCGGCACCTCAACATCAATGCCATCCGGGACTACT

CCTTCAAGAGGCTGTACCGACTCAAGGTCTTGGAGATCTCCCACTGGCCCTACTTGGACACCATGACACC

CAACTGCCTCTACGGCCTCAACCTGACGTCCCTGTCCATCACACACTGCAATCTGACCGCTGTGCCCTAC

CTGGCCGTCCGCCACCTAGTCTATCTCCGCTTCCTCAACCTCTCCTACAACCCCATCAGCACCATTGAGG

GCTCCATGTTGCATGAGCTGCTCCGGCTGCAGGAGATCCAGCTGGTGGGCGGGCAGCTGGCCGTGGTGGA

GCCCTATGCCTTCCGCGGCCTCAACTACCTGCGCGTGCTCAATGTCTCTGGCAACCAGCTGACCACACTG

GAGGAATCAGTCTTCCACTCGGTGGGCAACCTGGAGACACTCATCCTGGACTCCAACCCGCTGGCCTGCG

ACTGTCGGCTCCTGTGGGTGTTCCGGCGCCGCTGGCGGCTCAACTTCAACCGGCAGCAGCCCACGTGCGC

CACGCCCGAGTTTGTCCAGGGCAAGGAGTTCAAGGACTTCCCTGATGTGCTACTGCCCAACTACTTCACC

TGCCGCCGCGCCCGCATCCGGGACCGCAAGGCCCAGCAGGTGTTTGTGGACGAGGGCCACACGGTGCAGT

TTGTGTGCCGGGCCGATGGCGACCCGCCGCCCGCCATCCTCTGGCTCTCACCCCGAAAGCACCTGGTCTC

AGCCAAGAGCAATGGGCGGCTCACAGTCTTCCCTGATGGCACGCTGGAGGTGCGCTACGCCCAGGTACAG

GACAACGGCACGTACCTGTGCATCGCGGCCAACGCGGGCGGCAACGACTCCATGCCCGCCCACCTGCATG

TGCGCAGCTACTCGCCCGACTGGCCCCATCAGCCCAACAAGACCTTCGCTTTCATCTCCAACCAGCCGGG

CGAGGGAGAGGCCAACAGCACCCGCGCCACTGTGCCTTTCCCCTTCGACATCAAGACCCTCATCATCGCC

ACCACCATGGGCTTCATCTCTTTCCTGGGCGTCGTCCTCTTCTGCCTGGTGCTGCTGTTTCTCTGGAGCC

GGGGCAAGGGCAACACAAAGCACAACATCGAGATCGAGTATGTGCCCCGAAAGTCGGACGCAGGCATCAG

CTCCGCCGACGCGCCCCGCAAGTTCAACATGAAGATGATATGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund. 

 

References

 

1. Eur. J. Neurosci. 18:3167 (2003) 

2. Nat. Neurosci. 7:221-228 (2004) 

3. Nat. Neurosci. 8:745-751 (2005)

4. Neuron 45:345-351 (2005)

5. J. Biol. Chem. 281:36378 (2006) 

6. Proc. Natl. Acad. Sci. 104:14430 (2007)

 

  

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.