CD47, also known as IAP and OA3, is a variably glycosylated transmembrane protein of the immunoglobulin superfamily (IgSF). It contains an extracellular domain (ECD) with a single Ig-like domain, five transmembrane domains with short intervening loops and a 34 amino acid cytoplasmic tail at the C-terminus. CD47 is broadly expressed. It is involved in the increase in intracellular calcium concentration in response to cell adhesion to extracellular matrix. By acting as an adhesion receptor, CD47 plays a role in both cell adhesion by positively or negatively modulating Integrin-mediated function. CD47 also binds to the SIRP family members on macrophages, neutrophils, and T lymphocytes, preventing macrophage­mediated clearance of CD47­expressing cells and promoting immune cell transmigration across the vascular endothelium.  CD47 binds SIRPA which prevents maturation of dendritic cells and inhibits cytokine production. CD47 also interacts with SIRPG that enhances superantigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. CD47 is also a receptor for thrombospondin, and inhibits the angiogenic and vasorelaxant effects in the vasculature. On dendritic cells and myeloma cells, CD47 binds TSP-1 and induces giant cell formation and osteoclast differentiation.

CD47, also known as IAP and OA3, is a variably glycosylated transmembrane protein of the immunoglobulin superfamily (IgSF). It contains an extracellular domain (ECD) with a single Ig-like domain, five transmembrane domains with short intervening loops and a 34 amino acid cytoplasmic tail at the C-terminus. CD47 is broadly expressed. It is involved in the increase in intracellular calcium concentration in response to cell adhesion to extracellular matrix. By acting as an adhesion receptor, CD47 plays a role in both cell adhesion by positively or negatively modulating Integrin-mediated function. CD47 also binds to the SIRP family members on macrophages, neutrophils, and T lymphocytes, preventing macrophage­mediated clearance of CD47­expressing cells and promoting immune cell transmigration across the vascular endothelium.  CD47 binds SIRPA which prevents maturation of dendritic cells and inhibits cytokine production. CD47 also interacts with SIRPG that enhances superantigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation. CD47 is also a receptor for thrombospondin, and inhibits the angiogenic and vasorelaxant effects in the vasculature. On dendritic cells and myeloma cells, CD47 binds TSP-1 and induces giant cell formation and osteoclast differentiation.

 

Product Details

 

Gene Symbol: CD47; IAP; OA3; MER6

 

NCBI Gene ID: 961

 

Uniprot Entry: Q08722

 

Construct Details: Full length human CD47 gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 972 (bp)

 

Gene Insert Sequence:  

ATGTGGCCCCTGGTAGCGGCGCTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATA

AAACAAAATCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATGGA

GGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTACACCTTTGATGGA

GCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATTGAAGTCTCACAATTACTAAAAG

GAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTCTCACACACAGGAAACTACACTTGTGAAGTAAC

AGAATTAACCAGAGAAGGTGAAACGATCATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCAAAT

GAAAATATTCTTATTGTTATTTTCCCAATTTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAA

CACTTAAATATAGATCCGGTGGTATGGATGAGAAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCAC

TGTCATTGTCATTGTTGGAGCCATTCTTTTCGTCCCAGGTGAATATTCATTAAAGAATGCTACTGGCCTT

GGTTTAATTGTGACTTCTACAGGGATATTAATATTACTTCACTACTATGTGTTTAGTACAGCGATTGGAT

TAACCTCCTTCGTCATTGCCATATTGGTTATTCAGGTGATAGCCTATATCCTCGCTGTGGTTGGACTGAG

TCTCTGTATTGCGGCGTGTATACCAATGCATGGCCCTCTTCTGATTTCAGGTTTGAGTATCTTAGCTCTA

GCACAATTACTTGGACTAGTTTATATGAAATTTGTGGCTTCCAATCAGAAGACTATACAACCTCCTAGGA

AAGCTGTAGAGGAACCCCTTAATGCATTCAAAGAATCAAAAGGAATGATGAATGATGAATAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cancer Res. 52:5416 (1992).

2. Science 288:2051 (2000).

3. Trends Cell Biol. 11: 130 (2001).

4. J. Immunol. 168:5832 (2002).

5. Blood 109:836 (2007).

6. Curr. Drug Targ. 9:842 (2009).

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP2233-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.