VNN2 (vascular non-inflammatory molecule 2), also known as glycosyl- phosphatidyl inositol-anchored protein GPI-80, Vanin-2, and FOAP-4, is a GPI-anchored cell membrane protein belonging to the Vanin/BTD/VNN family of the carbon-nitrogen (CN) hydrolase family. The Vanin family includes secreted and membrane- associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. VNN2 shows high sequence similarity with other Vanin family members, and also with biotinidase. No biotinidase activity has been demonstrated for any of the vanin proteins.  VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. The pantetheinase activity of VNN2 may play a role in oxidative-stress response. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is involved in the thymus homing of bone marrow cells. It may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil. Elevated VNN2 expression is associated with development and progression of thymoma.

VNN2 (vascular non-inflammatory molecule 2), also known as glycosyl- phosphatidyl inositol-anchored protein GPI-80, Vanin-2, and FOAP-4, is a GPI-anchored cell membrane protein belonging to the Vanin/BTD/VNN family of the carbon-nitrogen (CN) hydrolase family. The Vanin family includes secreted and membrane- associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. VNN2 shows high sequence similarity with other Vanin family members, and also with biotinidase. No biotinidase activity has been demonstrated for any of the vanin proteins.  VNN2 is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. The pantetheinase activity of VNN2 may play a role in oxidative-stress response. VNN2 is widely expressed with higher expression in spleen and blood. VNN2 is involved in the thymus homing of bone marrow cells. It may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil. Elevated VNN2 expression is associated with development and progression of thymoma.

 

Product Details

 

Gene Symbol: VNN2; vanin 2; Vanin-2; FOAP-4; GPI-80

 

NCBI Gene ID: 8875

 

Uniprot Entry: Q95498

 

Construct Details: The recombinant human VNN2-Fc fusion is expressed as a 697 amino acid protein consisting of Gln23 -  Ser492 region of VNN2 (Uniprot Accession #O95498) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

 

Source:  Human cells stably expressing VNN2-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

QDSFIAAVYE HAVILPNKTE TPVSQEDALN LMNENIDILE TAIKQAAEQG ARIIVTPEDA 

        70         80         90        100        110        120 

LYGWKFTRET VFPYLEDIPD PQVNWIPCQD PHRFGHTPVQ ARLSCLAKDN SIYVLANLGD 

       130        140        150        160        170        180 

KKPCNSRDST CPPNGYFQYN TNVVYNTEGK LVARYHKYHL YSEPQFNVPE KPELVTFNTA 

       190        200        210        220        230        240 

FGRFGIFTCF DIFFYDPGVT LVKDFHVDTI LFPTAWMNVL PLLTAIEFHS AWAMGMGVNL  

       250        260        270        280        290        300 

LVANTHHVSL NMTGSGIYAP NGPKVYHYDM KTELGKLLLS EVDSHPLSSL AYPTAVNWNA 

       310        320        330        340        350        360 

YATTIKPFPV QKNTFRGFIS RDGFNFTELF ENAGNLTVCQ KELCCHLSYR MLQKEENEVY 

       370        380        390        400        410        420 

VLGAFTGLHG RRRREYWQVC TLLKCKTTNL TTCGRPVETA STRFEMFSLS GTFGTEYVFP 

       430        440        450        460        470        480 

EVLLTEIHLS PGKFEVLKDG RLVNKNGSSG PILTVSLFGR WYTKDSLYSS TGTHTCPPCP  

       490        500        510        520        530        540 

APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK 

       550        560        570        580        590        600 

PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT 

       610        620        630        640        650        660 

LPPSREEMTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL 

       670        680        690 

TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 78.7; Estimated by SDS-PAGE under reducing condition (kDa): 80-85

 

Calculated PI:  6.34 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm): 107690

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free).The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: VNN2 hydrolyzes D-pantetheine and releases cysteamine.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C for immediate use. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Genomics 53:203 (1998)

2. J. Immunol. 162:4277 (1999) 

3. Immunogenetics. 53:296 (2001)

4. Cancer Sci. 94:809 (2003)

5. J. Invest. Dermatol. 129: 2167 (2009)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL2117B-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.