MOG (myelin oligodendrocyte glycoprotein) is a two-pass type I transmembrane protein belonging to the BTN/MOG family of the immunoglobulin (Ig) superfamily.  MOG contains an Ig-like V-type domain in the first extracelluar region followed by two transmembrane regions and a small second extracellular tail. MOG is found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrogliocyte membranes. The MOG genes are highly conserved in mammals, suggesting an important biological role for this protein.  While the primary molecular function of MOG is not yet known, its likely role with the myelin sheath is either in sheath completion and/or maintenance.  MOG may mediate homophilic cell-cell adhesion. MOG is considered as an autoantigen capable to produce a demyelinating multiple sclerosis (MS)-like disease in experimental animals. Several studies have shown a role for antibodies against MOG in the pathogenesis of MS. The mutation in the MOG gene is associated with Narcolepsy 7 (NRCLP7), a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis.

MOG (myelin oligodendrocyte glycoprotein) is a two-pass type I transmembrane protein belonging to the BTN/MOG family of the immunoglobulin (Ig) superfamily.  MOG contains an Ig-like V-type domain in the first extracelluar region followed by two transmembrane regions and a small second extracellular tail. MOG is found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrogliocyte membranes. The MOG genes are highly conserved in mammals, suggesting an important biological role for this protein.  While the primary molecular function of MOG is not yet known, its likely role with the myelin sheath is either in sheath completion and/or maintenance.  MOG may mediate homophilic cell-cell adhesion. MOG is considered as an autoantigen capable to produce a demyelinating multiple sclerosis (MS)-like disease in experimental animals. Several studies have shown a role for antibodies against MOG in the pathogenesis of MS. The mutation in the MOG gene is associated with Narcolepsy 7 (NRCLP7), a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis.

 

Product Details

 

Gene Symbol: MOG; BTN6; BTNL11; MOGIG2; NRCLP7

 

NCBI Gene ID: 4340

 

Uniprot Entry: Q16653

 

Construct Details: Full length human MOG  gene is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 744 (bp)

 

Gene Insert Sequence:  

ATGGCAAGCTTATCAAGACCCTCTCTGCCCAGCTGCCTCTGCTCCTTCCTCCTCCTCCTCCTCCTCCAAGTGTCTTCCAG

CTATGCAGGGCAGTTCAGAGTGATAGGACCAAGACACCCTATCCGGGCTCTGGTCGGGGATGAAGTGGAATTGCCATGTC

GCATATCTCCTGGGAAGAACGCTACAGGCATGGAGGTGGGGTGGTACCGCCCCCCCTTCTCTAGGGTGGTTCATCTCTAC

AGAAATGGCAAGGACCAAGATGGAGACCAGGCACCTGAATATCGGGGCCGGACAGAGCTGCTGAAAGATGCTATTGGTGA

GGGAAAGGTGACTCTCAGGATCCGGAATGTAAGGTTCTCAGATGAAGGAGGTTTCACCTGCTTCTTCCGAGATCATTCTT

ACCAAGAGGAGGCAGCAATGGAATTGAAAGTAGAAGATCCTTTCTACTGGGTGAGCCCTGGAGTGCTGGTTCTCCTCGCG

GTGCTGCCTGTGCTCCTCCTGCAGATCACTGTTGGCCTCATCTTCCTCTGCCTGCAGTACAGACTGAGAGGAAAACTTCG

AGCAGAGATAGAGAATCTCCACCGGACTTTTGATCCCCACTTTCTGAGGGTGCCCTGCTGGAAGATAACCCTGTTTGTAA

TTGTGCCGGTTCTTGGACCCTTGGTTGCCTTGATCATCTGCTACAACTGGCTACATCGAAGACTAGCAGGGCAATTCCTT

GAAGAGCTACGAAATCCCTTCTGA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. J. Neurochem. 63:2353 (1993)

2. J. Neurochem. 65:309 (1994) 

3. J. Neurochem. 72: 1 (1999)

4. N. Engl. J. Med. 349: 139 (2003)

5. Am. J. Hum. Genet. 89:474 (2010)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: LTP1623-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.