DcR2 ( decoy receptor 2), also known as CD264, TRAILR4, and TRUNDD, is a Single-pass type I transmembrane protein of the TNF receptor superfamily (TNFRSF10D).  DcR2 contains an extracellular TRAIL-binding domain with 3 TNFR-cysteine repeats, a transmembrane domain, and a truncated cytoplasmic death domain. Therefore DcR2 does not induce apoptosis, and may play an inhibitory role in TRAIL-induced cell apoptosis. DcR2 is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. It is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. Aberrant methylation of DcR2 is frequent in multiple tumor types. Overexpression of TRAIL R4 can protect cells bearing TRAILR1 and/or TRAILR2 from TRAIL­mediated apoptosis. The human soluble DcR2-Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

DcR2 ( decoy receptor 2), also known as CD264, TRAILR4, and TRUNDD, is a Single-pass type I transmembrane protein of the TNF receptor superfamily (TNFRSF10D).  DcR2 contains an extracellular TRAIL-binding domain with 3 TNFR-cysteine repeats, a transmembrane domain, and a truncated cytoplasmic death domain. Therefore DcR2 does not induce apoptosis, and may play an inhibitory role in TRAIL-induced cell apoptosis. DcR2 is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. It is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. Aberrant methylation of DcR2 is frequent in multiple tumor types. Overexpression of TRAIL R4 can protect cells bearing TRAILR1 and/or TRAILR2 from TRAIL­mediated apoptosis. The human soluble DcR2-Fc chimera neutralizes the ability of TRAIL to induce apoptosis.

 

Product Details

 

Gene Symbol: DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4; UNQ251/PRO288

 

NCBI Gene ID: 8793

 

Uniprot Entry: Q9UBN6

 

Construct Details: The recombinant human DcR2-Fc fusion is expressed as a 382 amino acid protein consisting of Ala56 - Pro209 region of DcR2 (UniProt accession #Q9UBN6) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.  

 

Source:  Human cells stably expressing DcR2-Fc growing in chemical-defined media with no animal components or antibiotics 

 

Deduced Amino Acid Sequence:

        10         20         30         40         50         60 

ATIPRQDEVP QQTVAPQQQR RSLKEEECPA GSHRSEYTGA CNPCTEGVDY TIASNNLPSC 

        70         80         90        100        110        120 

LLCTVCKSGQ TNKSSCTTTR DTVCQCEKGS FQDKNSPEMC RTCRTGCPRG MVKVSNCTPR 

       130        140        150        160        170        180 

SDIKCKNESA ASSTGKTPAA EETVTTILGM LASPSTGTHT CPPCPAPELL GGPSVFLFPP 

       190        200        210        220        230        240 

KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV 

       250        260        270        280        290        300 

LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR EEMTKNQVSL 

       310        320        330        340        350        360 

TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC 

       370        380 

SVMHEALHNH YTQKSLSLSP GK 

 

M.W.: Calculated molecular mass (kDa): 42.1; Estimated by SDS-PAGE under reducing condition (kDa): 50-60

 

Calculated PI:  7.82 

 

Calculated Extinction Coefficient (M^-1 cm^-1, at 280nm):  39640

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as DTT "+")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBSpH7.4 (carrier and preservative free)

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand human TRAIL and inhibits TRAIL-mediated signaling activity including TRAIL­-induced cytotoxicity

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cur. Biol. 7:1003 (1997) 

2. Immunity 7:813 (1997)

3. Curr. Opin. Immunol. 10:559 (1998) 

4. FEBS lett 424:41 (1998) 

5. Mol Cell Biol. 26:7046 (2006)

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0474-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.