NGFR (nerve growth factor receptor), also known as Low affinity neurotrophin receptor p75NTR and CD271, is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF16).  The extracellular region contains four cysteine­rich domains and binds NGF, BDNF, NT­3 and NT­4 approximately equally with low affinity. The cytoplasmic region contains a death domain (DD). NGFR is widely expressed during development and in the adult. Expression has been detected in both neuronal and non­neuronal cells. NGFR was originally reported to function as a positive regulator of TrkA, a high- affinity nerve growth factor receptor. NGFR has also been shown to signal by itself. Depending on its cellular environment, NGFR has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and animals. NGFR is also necessary for the circadian oscillation of the clock genes in the suprachiasmatic nucleus of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. It may plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin and thereby contributes to the regulation of insulin-dependent glucose uptake.

NGFR (nerve growth factor receptor), also known as Low affinity neurotrophin receptor p75NTR and CD271, is a single-pass type I transmembrane glycoprotein in the TNF receptor superfamily (TNFRSF16).  The extracellular region contains four cysteine­rich domains and binds NGF, BDNF, NT­3 and NT­4 approximately equally with low affinity. The cytoplasmic region contains a death domain (DD). NGFR is widely expressed during development and in the adult. Expression has been detected in both neuronal and non­neuronal cells. NGFR was originally reported to function as a positive regulator of TrkA, a high- affinity nerve growth factor receptor. NGFR has also been shown to signal by itself. Depending on its cellular environment, NGFR has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and animals. NGFR is also necessary for the circadian oscillation of the clock genes in the suprachiasmatic nucleus of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. It may plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin and thereby contributes to the regulation of insulin-dependent glucose uptake.

  

Product Details

 

Gene Symbol: TNFRSF16; CD271; p75NTR; p75(NTR); Gp80-LNGFR; p75 ICD

  

NCBI Gene ID: 4804

 

Uniprot Entry: P08138

 

Construct Details: The recombinant human NGFR-Fc fusion is expressed as a 450 amino acid protein consisting of Lys29 -Asn250 region of NGFR (UniProt accession #P08138)  and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions (see the gel image inserted above).  

 

Source: Human cells stably expressing human NGFR-Fc and growing in chemical-defined media with no animal components or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60 

KEACPTGLYT HSGECCKACN LGEGVAQPCG ANQTVCEPCL DSVTFSDVVS ATEPCKPCTE  

        70         80         90        100        110        120 

CVGLQSMSAP CVEADDAVCR CAYGYYQDET TGRCEACRVC EAGSGLVFSC QDKQNTVCEE 

       130        140        150        160        170        180 

CPDGTYSDEA NHVDPCLPCT VCEDTERQLR ECTRWADAEC EEIPGRWITR STPPEGSDST 

       190        200        210        220        230        240 

APSTQEPEAP PEQDLIASTV AGVVTTVMGS SQPVVTRGTT DNSTGTHTCP PCPAPELLGG 

       250        260        270        280        290        300 

PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN 

       310        320        330        340        350        360 

STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSREE  

       370        380        390        400        410        420 

MTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW 

       430        440        450 

QQGNVFSCSV MHEALHNHYT QKSLSLSPGK 

 

M.W.: Calculated molecular mass (kDa): 49.1; Estimated by SDS-PAGE under reducing condition (kDa): 70-80

 

Calculated PI: 4.80

 

Calculated extinction coefficients (M-1 cm-1, at 280nm):  55735

 

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "DTT: +")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free).

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

Biological Activity: Binds to its ligand NGF and inhibits NGF dependent proliferation of  human TF-1 erythroleukemic cells. 

 

 

  

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks.

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

References

 

1. Cell 47:545-554 (1986).

2. J. Cell Biol. 140:911 (1998).

3. J Biol Chem. 279 (3): 1703 (2004).

4. Mol Biol Cell. 20 (16): 3620 (2009).

5. J. Neurosci. 33:10221 (2013). 

 

 

 

Documentation

 

Product datasheet (pdf) can be downloaded here: FCL0104-PDS.pdf

 

 

 

Additional supporting documents, including COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.