IgSF8 or CD316 is a 613 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the EWI subfamily of the Immunoglobulin superfamily (IgSF).  IgSF8 contains an N-terminal signal peptide, a 552-aa extracellular domain (ECD) with 4 Ig-like C2-type domains, an EWI (Glu-Trp-Ile)-motif and 3 potential glycosylation sites, a transmembrane domain (TM), and a 13-aa short cytoplasmic tail. IgSF8  interacts with the tetraspanins CD9 and CD81 and may regulate their role in certain cellular functions including cell migration, oocytes fertilization  and viral infection.  IGSF8 may regulate proliferation and differentiation of keratinocytes. IgSF8 may also function as a tumor suppressor by inhibiting the proliferation of certain cancers.  IgSF8 may be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell re-aggregation and motility. IgSF8 may also play a role on integrin-dependent morphology and motility functions. IgSF8 may participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.

IgSF8 or CD316 is a 613 amino acid (aa) single pass type I transmembrane glycoprotein that belongs to the EWI subfamily of the Immunoglobulin superfamily (IgSF).  IgSF8 contains an N-terminal signal peptide, a 552-aa extracellular domain (ECD) with 4 Ig-like C2-type domains, an EWI (Glu-Trp-Ile)-motif and 3 potential glycosylation sites, a transmembrane domain (TM), and a 13-aa short cytoplasmic tail. IgSF8  interacts with the tetraspanins CD9 and CD81 and may regulate their role in certain cellular functions including cell migration, oocytes fertilization  and viral infection.  IGSF8 may regulate proliferation and differentiation of keratinocytes. IgSF8 may also function as a tumor suppressor by inhibiting the proliferation of certain cancers.  IgSF8 may be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell re-aggregation and motility. IgSF8 may also play a role on integrin-dependent morphology and motility functions. IgSF8 may participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.

 

Product Details

 

Gene Symbol: IgSF8; CD316; EWI2; PGRL; EWI-2; KCT-4; CD81P3; LIR-D1

 

NCBI Gene ID: 93185

 

Uniprot Entry: Q969P0

 

Construct Details: The recombinant human IgSF8 ECD protein is expressed as a 573 amino acid protein consisting of Arg28 ­ Thr579 region of IgSF8 and a C-terminal His-tag.

   

Source: Human cells stably expressing IgSF8 ECD protein and growing in chemical-defined media with no animal component or antibiotics

 

Deduced Amino Acid Sequence: 

        10         20         30         40         50         60
REVLVPEGPL YRVAGTAVSI SCNVTGYEGP AQQNFEWFLY RPEAPDTALG IVSTKDTQFS

        70         80         90        100        110        120
YAVFKSRVVA GEVQVQRLQG DAVVLKIARL QAQDAGIYEC HTPSTDTRYL GSYSGKVELR

       130        140        150        160        170        180
VLPDVLQVSA APPGPRGRQA PTSPPRMTVH EGQELALGCL ARTSTQKHTH LAVSFGRSVP

       190        200        210        220        230        240
EAPVGRSTLQ EVVGIRSDLA VEAGAPYAER LAAGELRLGK EGTDRYRMVV GGAQAGDAGT

       250        260        270        280        290        300
YHCTAAEWIQ DPDGSWAQIA EKRAVLAHVD VQTLSSQLAV TVGPGERRIG PGEPLELLCN

       310        320        330        340        350        360
VSGALPPAGR HAAYSVGWEM APAGAPGPGR LVAQLDTEGV GSLGPGYEGR HIAMEKVASR

       370        380        390        400        410        420
TYRLRLEAAR PGDAGTYRCL AKAYVRGSGT RLREAASARS RPLPVHVREE GVVLEAVAWL

       430        440        450        460        470        480
AGGTVYRGET ASLLCNISVR GGPPGLRLAA SWWVERPEDG ELSSVPAQLV GGVGQDGVAE

       490        500        510        520        530        540
LGVRPGGGPV SVELVGPRSH RLRLHSLGPE DEGVYHCAPS AWVQHADYSW YQAGSARSGP

       550        560        570
VTVYPYMHAL DTSTGYPYDV PDYAGHHHHH HHH

 

 

M.W.: Calculated molecular mass 61 kDa; estimated by SDS-PAGE under reducing condition 65-70 kDa

 

Calculated PI: 6.54

 

Calculated extinction coefficients (M-1 cm-1, at 280nm): 85760

  

Purity: >95% judged by SDS-PAGE under reducing condition (see the gel image above, labeled as "S")

 

Formulation: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (concentration determined by UV spectrometry and verified by SDS-PAGE and Coomassie blue staining).  The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

 

Endotoxin Level: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method

 

 

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.