LGR6 (Leucine-rich repeat-containing G-protein coupled Receptor 6) is a member of the G-protein coupled receptor 1 (GPCR1) family of 7-pass transmembrane proteins. LGR6 contains a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding.  LGR6 is the receptor for R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4) that potentiates the canonical Wnt signaling pathway.  In contrast to classical G-protein coupled receptors, LGR6 does not activate heterotrimeric G-proteins to transduce the signal. Upon binding to R-spondins, LGR6 associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. LGR6 is involved in the development and maintenance of the adult intestinal stem cells.  LGR6 is a marker of multipotent stem cells in the epidermis and may also act as a tumor suppressor.

LGR6 (Leucine-rich repeat-containing G-protein coupled Receptor 6) is a member of the G-protein coupled receptor 1 (GPCR1) family of 7-pass transmembrane proteins. LGR6 contains a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding.  LGR6 is the receptor for R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4) that potentiates the canonical Wnt signaling pathway.  In contrast to classical G-protein coupled receptors, LGR6 does not activate heterotrimeric G-proteins to transduce the signal. Upon binding to R-spondins, LGR6 associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. LGR6 is involved in the development and maintenance of the adult intestinal stem cells.  LGR6 is a marker of multipotent stem cells in the epidermis and may also act as a tumor suppressor.

 

Product Details

 

Gene Symbol: LGR6; VTS20631

 

NCBI Gene ID: 59352

 

Uniprot Entry: Q9HBX8

 

Construct Details: Full length human LGR6 gene (encoding Uniprot# Q9HBX8-3) is subcloned into the Lentiviral expression vector pLTC with an upstream CMV promoter and with or without a selection marker, which can be used for both transient and stable expression in mammalian cells.  It can be co-transfected with the LentiPAK DNA mix (SKU# LP-001) into HEK293 cells to produce high titer lentiviral particles. Multiple choices of a stable antibiotic selection marker are available.

 

Vector Type: pLTC or pLTC-IRES-Marker (lentiviral expression vector containing a heterologous CMV promoter, see the vector map above)

 

Formulation: Supplied with 10 μg of endotoxin-free plasmid DNA at 200 ng/μl in sterile TE buffer (pH8.0)

 

Gene Insert Size: 2904 (bp)

 

Gene Insert Sequence:  

ATGCCCAGCCCGCCGGGGCTCCGGGCGCTATGGCTTTGCGCCGCGCTGTGCGCTTCCCGGAGGGCCGGCG

GCGCCCCCCAGCCCGGCCCGGGGCCCACCGCCTGCCCGGCCCCCTGCCACTGCCAGGAGGACGGCATCAT

GCTGTCTGCCGACTGCTCTGAGCTCGGGCTGTCCGCCGTTCCGGGGGACCTGGACCCCCTGACGGCTTAC

CTGGACCTCAGCATGAACAACCTCACAGAGCTTCAGCCTGGCCTCTTCCACCACCTGCGCTTCTTGGAGG

AGCTGCGTCTCTCTGGGAACCATCTCTCACACATCCCAGGACAAGCATTCTCTGGTCTCTACAGCCTGAA

AATCCTGATGCTGCAGAACAATCAGCTGGGAGGAATCCCCGCAGAGGCGCTGTGGGAGCTGCCGAGCCTG

CAGTCGCTGCGCCTAGATGCCAACCTCATCTCCCTGGTCCCGGAGAGGAGCTTTGAGGGGCTGTCCTCCC

TCCGCCACCTCTGGCTGGACGACAATGCACTCACGGAGATCCCTGTCAGGGCCCTCAACAACCTCCCTGC

CCTGCAGGCCATGACCCTGGCCCTCAACCGCATCAGCCACATCCCCGACTACGCGTTCCAGAATCTCACC

AGCCTTGTGGTGCTGCATTTGCATAACAACCGCATCCAGCATCTGGGGACCCACAGCTTCGAGGGGCTGC

ACAATCTGGAGACACTAGACCTGAATTATAACAAGCTGCAGGAGTTCCCTGTGGCCATCCGGACCCTGGG

CAGACTGCAGGAACTGGGGTTCCATAACAACAACATCAAGGCCATCCCAGAAAAGGCCTTCATGGGGAAC

CCTCTGCTACAGACGATACACTTTTATGATAACCCAATCCAGTTTGTGGGAAGATCGGCATTCCAGTACC

TGCCTAAACTCCACACACTATCTCTGAATGGTGCCATGGACATCCAGGAGTTTCCAGATCTCAAAGGCAC

CACCAGCCTGGAGATCCTGACCCTGACCCGCGCAGGCATCCGGCTGCTCCCATCGGGGATGTGCCAACAG

CTGCCCAGGCTCCGAGTCCTGGAACTGTCTCACAATCAAATTGAGGAGCTGCCCAGCCTGCACAGGTGTC

AGAAATTGGAGGAAATCGGCCTCCAACACAACCGCATCTGGGAAATTGGAGCTGACACCTTCAGCCAGCT

GAGCTCCCTGCAAGCCCTGGATCTTAGCTGGAACGCCATCCGGTCCATCCACCCCGAGGCCTTCTCCACC

CTGCACTCCCTGGTCAAGCTGGACCTGACAGACAACCAGCTGACCACACTGCCCCTGGCTGGACTTGGGG

GCTTGATGCATCTGAAGCTCAAAGGGAACCTTGCTCTCTCCCAGGCCTTCTCCAAGGACAGTTTCCCAAA

ACTGAGGATCCTGGAGGTGCCTTATGCCTACCAGTGCTGTCCCTATGGGATGTGTGCCAGCTTCTTCAAG

GCCTCTGGGCAGTGGGAGGCTGAAGACCTTCACCTTGATGATGAGGAGTCTTCAAAAAGGCCCCTGGGCC

TCCTTGCCAGACAAGCAGAGAACCACTATGACCAGGACCTGGATGAGCTCCAGCTGGAGATGGAGGACTC

AAAGCCACACCCCAGTGTCCAGTGTAGCCCTACTCCAGGCCCCTTCAAGCCCTGTGAGTACCTCTTTGAA

AGCTGGGGCATCCGCCTGGCCGTGTGGGCCATCGTGTTGCTCTCCGTGCTCTGCAATGGACTGGTGCTGC

TGACCGTGTTCGCTGGCGGGCCTGTCCCCCTGCCCCCGGTCAAGTTTGTGGTAGGTGCGATTGCAGGCGC

CAACACCTTGACTGGCATTTCCTGTGGCCTTCTAGCCTCAGTCGATGCCCTGACCTTTGGTCAGTTCTCT

GAGTACGGAGCCCGCTGGGAGACGGGGCTAGGCTGCCGGGCCACTGGCTTCCTGGCAGTACTTGGGTCGG

AGGCATCGGTGCTGCTGCTCACTCTGGCCGCAGTGCAGTGCAGCGTCTCCGTCTCCTGTGTCCGGGCCTA

TGGGAAGTCCCCCTCCCTGGGCAGCGTTCGAGCAGGGGTCCTAGGCTGCCTGGCACTGGCAGGGCTGGCC

GCCGCGCTGCCCCTGGCCTCAGTGGGAGAATACGGGGCCTCCCCACTCTGCCTGCCCTACGCGCCACCTG

AGGGTCAGCCAGCAGCCCTGGGCTTCACCGTGGCCCTGGTGATGATGAACTCCTTCTGTTTCCTGGTCGT

GGCCGGTGCCTACATCAAACTGTACTGTGACCTGCCGCGGGGCGACTTTGAGGCCGTGTGGGACTGCGCC

ATGGTGAGGCACGTGGCCTGGCTCATCTTCGCAGACGGGCTCCTCTACTGTCCCGTGGCCTTCCTCAGCT

TTGCCTCCATGCTGGGCCTCTTCCCTGTCACGCCCGAGGCCGTCAAGTCTGTCCTGCTGGTGGTGCTGCC

CCTGCCTGCCTGCCTCAACCCACTGCTGTACCTGCTCTTCAACCCCCACTTCCGGGATGACCTTCGGCGG

CTTCGGCCCCGCGCAGGGGACTCAGGGCCCCTAGCCTATGCTGCGGCCGGGGAGCTGGAGAAGAGCTCCT

GTGATTCTACCCAGGCCCTGGTAGCCTTCTCTGATGTGGATCTCATTCTGGAAGCTTCTGAAGCTGGGCG

GCCCCCTGGGCTGGAGACCTATGGCTTCCCCTCAGTGACCCTCATCTCCTGTCAGCAGCCAGGGGCCCCC

AGGCTGGAGGGCAGCCATTGTGTAGAGCCAGAGGGGAACCACTTTGGGAACCCCCAACCCTCCATGGATG

GAGAACTGCTGCTGAGGGCAGAGGGATCTACGCCAGCAGGTGGAGGCTTGTCAGGGGGTGGCGGCTTTCA

GCCCTCTGGCTTGGCCTTTGCTTCACACGTGTAA

 

 

Primers: Gene insert coding sequence can be confirmed by following primers:

(a) forward (or 5'-end) primer: 5’-CAAATGGGCGGTAGGCGTG-3’;

(b) reverse (or 3'-end) primer: 5’-CCAGGTTTCCGGGCCCTCAC-3’

 

 

Note: The sequence may vary due to the naturally occurring variants or mutations, such as single nucleotide polymorphism, or the artifact during PCR amplification.  High fidelity PCR systems should always be used. 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

Storage

 

The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 6 months. 

 

 

 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.

 

References

 

 

 

Additional supporting documents, including product datasheet, COA and MSDS are available upon request.

 

 

 

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE IN HUMAN.

Restriction: This product is not transferable or re-sellable.  Customer obtain no right to transfer, assign, or sublicense its use rights, or to transfer, resell, package, or otherwise distribute the product, or to use the product for the benefit of any third party or for any commercial purpose.  Customer may only use the product in compliance with applicable local, state and federal laws, regulations and rules.  Customer may not directly or indirectly use the product or allow the transfer, transmission, export or re-export of all or any part of the product in violation of any export control law or regulation of the united states or any other relevant jurisdiction.  Your use of this product constitutes acceptance of the terms of this limited use agreement.  Please refer to our “terms & conditions” for details.  If you are not willing to accept the limitation of this agreement, G&P Biosciences will accept return of the product for a full/partial refund.